Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes
In mice, monocytes (Mo) are conventionally described as CX3CR1low classical Mo (CMo) and CX3CR1high non-classical Mo (NCMo) based on the expression of EGFP in Cx3cr1+/EGFP mice and by analogy with human CX3CR1 expression. Although this terminology is widely used, it may not reflect the expression of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.01117/full |
| _version_ | 1818995884879249408 |
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| author | Aïda Meghraoui-Kheddar Aïda Meghraoui-Kheddar Sandrine Barthelemy Alexandre Boissonnas Christophe Combadière |
| author_facet | Aïda Meghraoui-Kheddar Aïda Meghraoui-Kheddar Sandrine Barthelemy Alexandre Boissonnas Christophe Combadière |
| author_sort | Aïda Meghraoui-Kheddar |
| collection | DOAJ |
| description | In mice, monocytes (Mo) are conventionally described as CX3CR1low classical Mo (CMo) and CX3CR1high non-classical Mo (NCMo) based on the expression of EGFP in Cx3cr1+/EGFP mice and by analogy with human CX3CR1 expression. Although this terminology is widely used, it may not reflect the expression of CX3CR1 on Mo subsets. Using an unsupervised multiparametric analysis of blood Mo in steady state and after sterile peritonitis, we observed that CX3CR1 expression did not discriminate the CMo from the NCMo subsets. Our results highlight that despite being a reliable reporter to discriminate Mo subpopulations, EGFP level in Cx3cr1+/EGFP mice does not reflect CX3CR1 expression measured by a fluorescently-labeled CX3CL1 chemokine and a CX3CR1 specific antibody. In conclusion, authors should be cautious not to identify murine classical and non-classical Mo as CX3CR1low and CX3CR1high but rather use alternative markers such as the combination of Ly6C and CD43. |
| first_indexed | 2024-12-20T21:20:57Z |
| format | Article |
| id | doaj.art-b351eb5f4ef94cc6af5a882720483838 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T21:20:57Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-b351eb5f4ef94cc6af5a8827204838382022-12-21T19:26:17ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-06-011110.3389/fimmu.2020.01117544632Revising CX3CR1 Expression on Murine Classical and Non-classical MonocytesAïda Meghraoui-Kheddar0Aïda Meghraoui-Kheddar1Sandrine Barthelemy2Alexandre Boissonnas3Christophe Combadière4Sorbonne Université, Inserm, CNRS, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, Paris, FranceUniversité Côte d'Azur, CNRS UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire (IPMC), Valbonne, FranceSorbonne Université, Inserm, CNRS, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, Paris, FranceSorbonne Université, Inserm, CNRS, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, Paris, FranceSorbonne Université, Inserm, CNRS, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, Paris, FranceIn mice, monocytes (Mo) are conventionally described as CX3CR1low classical Mo (CMo) and CX3CR1high non-classical Mo (NCMo) based on the expression of EGFP in Cx3cr1+/EGFP mice and by analogy with human CX3CR1 expression. Although this terminology is widely used, it may not reflect the expression of CX3CR1 on Mo subsets. Using an unsupervised multiparametric analysis of blood Mo in steady state and after sterile peritonitis, we observed that CX3CR1 expression did not discriminate the CMo from the NCMo subsets. Our results highlight that despite being a reliable reporter to discriminate Mo subpopulations, EGFP level in Cx3cr1+/EGFP mice does not reflect CX3CR1 expression measured by a fluorescently-labeled CX3CL1 chemokine and a CX3CR1 specific antibody. In conclusion, authors should be cautious not to identify murine classical and non-classical Mo as CX3CR1low and CX3CR1high but rather use alternative markers such as the combination of Ly6C and CD43.https://www.frontiersin.org/article/10.3389/fimmu.2020.01117/fullmonocyteschemokine receptorCX3CR1CD43multiparametric analysis |
| spellingShingle | Aïda Meghraoui-Kheddar Aïda Meghraoui-Kheddar Sandrine Barthelemy Alexandre Boissonnas Christophe Combadière Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes Frontiers in Immunology monocytes chemokine receptor CX3CR1 CD43 multiparametric analysis |
| title | Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes |
| title_full | Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes |
| title_fullStr | Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes |
| title_full_unstemmed | Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes |
| title_short | Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes |
| title_sort | revising cx3cr1 expression on murine classical and non classical monocytes |
| topic | monocytes chemokine receptor CX3CR1 CD43 multiparametric analysis |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.01117/full |
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