Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research Hospital
Introduction: In the diagnosis of bacteremia and sepsis, blood culture is the gold standard. Determining the distribution and antibiotic sensitivity of the microorganisms isolated from blood cultures is very important in reducing mortality and morbidity. In this study, it was aimed to investigate th...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bilimsel Tip Yayinevi
2016-03-01
|
| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2016-21-01-027-032.pdf |
| _version_ | 1797908635244822528 |
|---|---|
| author | Leyla TAŞÇI A. Semra GÜRESER Z. İlkay BOYACIOĞLU Djursun KARASARTOVA Ayşegül TAYLAN ÖZKAN |
| author_facet | Leyla TAŞÇI A. Semra GÜRESER Z. İlkay BOYACIOĞLU Djursun KARASARTOVA Ayşegül TAYLAN ÖZKAN |
| author_sort | Leyla TAŞÇI |
| collection | DOAJ |
| description | Introduction: In the diagnosis of bacteremia and sepsis, blood culture is the gold standard. Determining the distribution and antibiotic sensitivity of the microorganisms isolated from blood cultures is very important in reducing mortality and morbidity. In this study, it was aimed to investigate the blood cultures sent to our laboratory from our hospital’s intensive care unit and other services and the distribution of isolated microorganisms, extended spectrum beta-lactamase (ESBL) positivity and the susceptibility to various antibiotics.
Materials and Methods: Between 01.01.2014 and 31.03.2015, 3100 blood cultures taken from patients were incubated in automated blood culture system (BACT/ALERT 3D, bioMerieux, France) in the Microbiology Laboratory of Hitit University Corum Training and Research Hospital. From signal received bottles, inoculations were made on to blood agar, eosin methylene blue (EMB) and chocolate agar. Plates were incubated at 37°C for 18-24 hours and microorganisms such as Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus spp.) and antibiotic susceptibilities were determined in VITEK 2 (bioMerieux, France) fully automated bacterial identification device.
Results: Among the 224 strains isolated from blood cultures, 80 (35.7%) of them were gram-positive and 144 (64.3%) of them were gram negative bacteria. ESBL-positive strains of E. coli and K. pneumoniae strains were found as 37.2%, 29.4%, respectively. 25% of S. aureus strains was found to be resistant to methicillin. S. aureus and Enterococcus spp. strains were highly sensitive to tigecycline, vancomycin and teicoplanin, while Enterobacteriaceae to carbapenem, colistin and aminoglycosides.
Conclusion: Isolation, identification and determination of antibiotic sensitivity of the microorganisms cultivated in blood cultures give a lead in terms of empiric treatment. Since ESBL producing bacteria are multi drug resistant, broad spectrum antibiotics are needed to be used. |
| first_indexed | 2024-04-10T10:56:44Z |
| format | Article |
| id | doaj.art-b354439f0c1549c29b902a87911b4f57 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T10:56:44Z |
| publishDate | 2016-03-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-b354439f0c1549c29b902a87911b4f572023-02-15T16:19:58ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2016-03-012112732Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research HospitalLeyla TAŞÇI0A. Semra GÜRESER1Z. İlkay BOYACIOĞLU2Djursun KARASARTOVA3Ayşegül TAYLAN ÖZKAN4Laboratory of Microbiology, Hitit University Corum Training and Research Hospital, Corum, TurkeyLaboratory of Microbiology, Hitit University Corum Training and Research Hospital, Corum, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, University of Hitit, Corum, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, University of Hitit, Corum, TurkeyLaboratory of Microbiology, Hitit University Corum Training and Research Hospital, Corum, TurkeyIntroduction: In the diagnosis of bacteremia and sepsis, blood culture is the gold standard. Determining the distribution and antibiotic sensitivity of the microorganisms isolated from blood cultures is very important in reducing mortality and morbidity. In this study, it was aimed to investigate the blood cultures sent to our laboratory from our hospital’s intensive care unit and other services and the distribution of isolated microorganisms, extended spectrum beta-lactamase (ESBL) positivity and the susceptibility to various antibiotics. Materials and Methods: Between 01.01.2014 and 31.03.2015, 3100 blood cultures taken from patients were incubated in automated blood culture system (BACT/ALERT 3D, bioMerieux, France) in the Microbiology Laboratory of Hitit University Corum Training and Research Hospital. From signal received bottles, inoculations were made on to blood agar, eosin methylene blue (EMB) and chocolate agar. Plates were incubated at 37°C for 18-24 hours and microorganisms such as Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus spp.) and antibiotic susceptibilities were determined in VITEK 2 (bioMerieux, France) fully automated bacterial identification device. Results: Among the 224 strains isolated from blood cultures, 80 (35.7%) of them were gram-positive and 144 (64.3%) of them were gram negative bacteria. ESBL-positive strains of E. coli and K. pneumoniae strains were found as 37.2%, 29.4%, respectively. 25% of S. aureus strains was found to be resistant to methicillin. S. aureus and Enterococcus spp. strains were highly sensitive to tigecycline, vancomycin and teicoplanin, while Enterobacteriaceae to carbapenem, colistin and aminoglycosides. Conclusion: Isolation, identification and determination of antibiotic sensitivity of the microorganisms cultivated in blood cultures give a lead in terms of empiric treatment. Since ESBL producing bacteria are multi drug resistant, broad spectrum antibiotics are needed to be used.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2016-21-01-027-032.pdfBlood cultureAntimicrobial susceptibility |
| spellingShingle | Leyla TAŞÇI A. Semra GÜRESER Z. İlkay BOYACIOĞLU Djursun KARASARTOVA Ayşegül TAYLAN ÖZKAN Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research Hospital Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Blood culture Antimicrobial susceptibility |
| title | Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research Hospital |
| title_full | Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research Hospital |
| title_fullStr | Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research Hospital |
| title_full_unstemmed | Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research Hospital |
| title_short | Microorganisms Isolated from Blood Cultures and Their Antimicrobial Susceptibility in Hitit University Corum Training and Research Hospital |
| title_sort | microorganisms isolated from blood cultures and their antimicrobial susceptibility in hitit university corum training and research hospital |
| topic | Blood culture Antimicrobial susceptibility |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2016-21-01-027-032.pdf |
| work_keys_str_mv | AT leylatasci microorganismsisolatedfrombloodculturesandtheirantimicrobialsusceptibilityinhitituniversitycorumtrainingandresearchhospital AT asemragureser microorganismsisolatedfrombloodculturesandtheirantimicrobialsusceptibilityinhitituniversitycorumtrainingandresearchhospital AT zilkayboyacioglu microorganismsisolatedfrombloodculturesandtheirantimicrobialsusceptibilityinhitituniversitycorumtrainingandresearchhospital AT djursunkarasartova microorganismsisolatedfrombloodculturesandtheirantimicrobialsusceptibilityinhitituniversitycorumtrainingandresearchhospital AT aysegultaylanozkan microorganismsisolatedfrombloodculturesandtheirantimicrobialsusceptibilityinhitituniversitycorumtrainingandresearchhospital |