Comparison of NET quantification methods based on immunofluorescence microscopy: Hand-counting, semi-automated and automated evaluations

Comparison of NET quantification methods based on immunofluorescence microscopy: Hand-counting, semi-automated and automated evaluations

Formation of neutrophil extracellular traps was first described in 2004, showing that NETs are composed of decondensed chromatin fibers and nuclear and granule components. Free DNA is often used to quantify NETs, but to differentiate NETosis from necrotic DNA-release, immunofluorescence microscopy w...

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Bibliographic Details
Main Authors: Timo Henneck, Christina Krüger, Andreas Nerlich, Melissa Langer, Leonie Fingerhut, Marta C. Bonilla, Marita Meurer, Sönke von den Berg, Nicole de Buhr, Katja Branitzki-Heinemann, Maren von Köckritz-Blickwede
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Heliyon
Subjects:
NETs
ImageJ
Quantification
Cell counting
NETQUANT
In vitro
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844023041890

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