Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration
Glioblastoma (GB) is the most common and the most aggressive form of brain tumor in adults, which currently lacks efficient treatment strategies. In this study, we investigated the therapeutic effect of function-blocking antibodies targeting integrin α10β1 on patient-derived-GB cell lines in vitro a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/5/1184 |
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| author | Katarzyna Chmielarska Masoumi Xiaoli Huang Wondossen Sime Anna Mirkov Matilda Munksgaard Thorén Ramin Massoumi Evy Lundgren-Åkerlund |
| author_facet | Katarzyna Chmielarska Masoumi Xiaoli Huang Wondossen Sime Anna Mirkov Matilda Munksgaard Thorén Ramin Massoumi Evy Lundgren-Åkerlund |
| author_sort | Katarzyna Chmielarska Masoumi |
| collection | DOAJ |
| description | Glioblastoma (GB) is the most common and the most aggressive form of brain tumor in adults, which currently lacks efficient treatment strategies. In this study, we investigated the therapeutic effect of function-blocking antibodies targeting integrin α10β1 on patient-derived-GB cell lines in vitro and in vivo. The in vitro studies demonstrated significant inhibiting effects of the integrin α10 antibodies on the adhesion, migration, proliferation, and sphere formation of GB cells. In a xenograft mouse model, the effect of the antibodies on tumor growth was investigated in luciferase-labeled and subcutaneously implanted GB cells. As demonstrated by in vivo imaging analysis and caliper measurements, the integrin α10-antibodies significantly suppressed GB tumor growth compared to control antibodies. Immunohistochemical analysis of the GB tumors showed lower expression of the proliferation marker Ki67 and an increased expression of cleaved caspase-3 after treatment with integrin α10 antibodies, further supporting a therapeutic effect. Our results suggest that function-blocking antibody targeting integrin α10β1 is a promising therapeutic strategy for the treatment of glioblastoma. |
| first_indexed | 2024-03-10T13:24:28Z |
| format | Article |
| id | doaj.art-b3581f489cb440efbe16fd4a0fc34e8d |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T13:24:28Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-b3581f489cb440efbe16fd4a0fc34e8d2023-11-21T09:46:01ZengMDPI AGCancers2072-66942021-03-01135118410.3390/cancers13051184Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell MigrationKatarzyna Chmielarska Masoumi0Xiaoli Huang1Wondossen Sime2Anna Mirkov3Matilda Munksgaard Thorén4Ramin Massoumi5Evy Lundgren-Åkerlund6Xintela AB, Medicon Village, Scheeletorget 1, SE-223 81 Lund, SwedenXintela AB, Medicon Village, Scheeletorget 1, SE-223 81 Lund, SwedenIVRS AB, Medicon Village, Scheeletorget 1, SE-223 81 Lund, SwedenXintela AB, Medicon Village, Scheeletorget 1, SE-223 81 Lund, SwedenXintela AB, Medicon Village, Scheeletorget 1, SE-223 81 Lund, SwedenIVRS AB, Medicon Village, Scheeletorget 1, SE-223 81 Lund, SwedenXintela AB, Medicon Village, Scheeletorget 1, SE-223 81 Lund, SwedenGlioblastoma (GB) is the most common and the most aggressive form of brain tumor in adults, which currently lacks efficient treatment strategies. In this study, we investigated the therapeutic effect of function-blocking antibodies targeting integrin α10β1 on patient-derived-GB cell lines in vitro and in vivo. The in vitro studies demonstrated significant inhibiting effects of the integrin α10 antibodies on the adhesion, migration, proliferation, and sphere formation of GB cells. In a xenograft mouse model, the effect of the antibodies on tumor growth was investigated in luciferase-labeled and subcutaneously implanted GB cells. As demonstrated by in vivo imaging analysis and caliper measurements, the integrin α10-antibodies significantly suppressed GB tumor growth compared to control antibodies. Immunohistochemical analysis of the GB tumors showed lower expression of the proliferation marker Ki67 and an increased expression of cleaved caspase-3 after treatment with integrin α10 antibodies, further supporting a therapeutic effect. Our results suggest that function-blocking antibody targeting integrin α10β1 is a promising therapeutic strategy for the treatment of glioblastoma.https://www.mdpi.com/2072-6694/13/5/1184function-blocking antibodyglioblastomaintegrin α10β1collagenmigrationtumor growth |
| spellingShingle | Katarzyna Chmielarska Masoumi Xiaoli Huang Wondossen Sime Anna Mirkov Matilda Munksgaard Thorén Ramin Massoumi Evy Lundgren-Åkerlund Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration Cancers function-blocking antibody glioblastoma integrin α10β1 collagen migration tumor growth |
| title | Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration |
| title_full | Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration |
| title_fullStr | Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration |
| title_full_unstemmed | Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration |
| title_short | Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration |
| title_sort | integrin α10 antibodies reduce glioblastoma tumor growth and cell migration |
| topic | function-blocking antibody glioblastoma integrin α10β1 collagen migration tumor growth |
| url | https://www.mdpi.com/2072-6694/13/5/1184 |
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