Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia
Abstract The prevalence of antibiotic-resistant bacteria in Southeast Asia is a significant concern, yet there is limited research on the gut resistome and its correlation with lifestyle and environmental factors in the region. This study aimed to profile the gut resistome of 200 individuals in Mala...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-023-00425-0 |
| _version_ | 1797577767857946624 |
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| author | J. Dwiyanto M. A. L. Huët M. H. Hussain T. T. Su J. B. L. Tan K. Y. Toh J. W. J. Lee S. Rahman C. W. Chong |
| author_facet | J. Dwiyanto M. A. L. Huët M. H. Hussain T. T. Su J. B. L. Tan K. Y. Toh J. W. J. Lee S. Rahman C. W. Chong |
| author_sort | J. Dwiyanto |
| collection | DOAJ |
| description | Abstract The prevalence of antibiotic-resistant bacteria in Southeast Asia is a significant concern, yet there is limited research on the gut resistome and its correlation with lifestyle and environmental factors in the region. This study aimed to profile the gut resistome of 200 individuals in Malaysia using shotgun metagenomic sequencing and investigate its association with questionnaire data comprising demographic and lifestyle variables. A total of 1038 antibiotic resistance genes from 26 classes were detected with a mean carriage rate of 1.74 ± 1.18 gene copies per cell per person. Correlation analysis identified 14 environmental factors, including hygiene habits, health parameters, and intestinal colonization, that were significantly associated with the resistome (adjusted multivariate PERMANOVA, p < 0.05). Notably, individuals with positive yeast cultures exhibited a reduced copy number of 15 antibiotic resistance genes. Network analysis highlighted Escherichia coli as a major resistome network hub, with a positive correlation to 36 antibiotic-resistance genes. Our findings suggest that E. coli may play a pivotal role in shaping the resistome dynamics in Segamat, Malaysia, and its abundance is strongly associated with the community’s health and lifestyle habits. Furthermore, the presence of yeast appears to be associated with the suppression of antibiotic-resistance genes. |
| first_indexed | 2024-03-10T22:13:37Z |
| format | Article |
| id | doaj.art-b35d4a8b92264316b952f81a222678d2 |
| institution | Directory Open Access Journal |
| issn | 2055-5008 |
| language | English |
| last_indexed | 2024-03-10T22:13:37Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj.art-b35d4a8b92264316b952f81a222678d22023-11-19T12:32:11ZengNature Portfolionpj Biofilms and Microbiomes2055-50082023-08-019111110.1038/s41522-023-00425-0Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern MalaysiaJ. Dwiyanto0M. A. L. Huët1M. H. Hussain2T. T. Su3J. B. L. Tan4K. Y. Toh5J. W. J. Lee6S. Rahman7C. W. Chong8AMILIFaculty of Science, University of MauritiusSchool of Science, Monash University MalaysiaSouth East Asia Community ObservatorySchool of Science, Monash University MalaysiaAMILIAMILISchool of Science, Monash University MalaysiaAMILIAbstract The prevalence of antibiotic-resistant bacteria in Southeast Asia is a significant concern, yet there is limited research on the gut resistome and its correlation with lifestyle and environmental factors in the region. This study aimed to profile the gut resistome of 200 individuals in Malaysia using shotgun metagenomic sequencing and investigate its association with questionnaire data comprising demographic and lifestyle variables. A total of 1038 antibiotic resistance genes from 26 classes were detected with a mean carriage rate of 1.74 ± 1.18 gene copies per cell per person. Correlation analysis identified 14 environmental factors, including hygiene habits, health parameters, and intestinal colonization, that were significantly associated with the resistome (adjusted multivariate PERMANOVA, p < 0.05). Notably, individuals with positive yeast cultures exhibited a reduced copy number of 15 antibiotic resistance genes. Network analysis highlighted Escherichia coli as a major resistome network hub, with a positive correlation to 36 antibiotic-resistance genes. Our findings suggest that E. coli may play a pivotal role in shaping the resistome dynamics in Segamat, Malaysia, and its abundance is strongly associated with the community’s health and lifestyle habits. Furthermore, the presence of yeast appears to be associated with the suppression of antibiotic-resistance genes.https://doi.org/10.1038/s41522-023-00425-0 |
| spellingShingle | J. Dwiyanto M. A. L. Huët M. H. Hussain T. T. Su J. B. L. Tan K. Y. Toh J. W. J. Lee S. Rahman C. W. Chong Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia npj Biofilms and Microbiomes |
| title | Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia |
| title_full | Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia |
| title_fullStr | Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia |
| title_full_unstemmed | Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia |
| title_short | Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia |
| title_sort | social demographics determinants for resistome and microbiome variation of a multiethnic community in southern malaysia |
| url | https://doi.org/10.1038/s41522-023-00425-0 |
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