Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor
In tissue engineering field, it is important to develop a suitable numerical model to evaluate scaffold geometry design. The experimental evaluation of the effect of each specific scaffold parameter on tissue regeneration requires large cost and long time expend. Dynamic cell culture is commonly use...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Medicine in Novel Technology and Devices |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590093521000485 |
| _version_ | 1818356257702019072 |
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| author | Ziyu Liu Chunjing Tao Shanshan Yuan Wei Wang Maryam Tamaddon Liqi Ng Hao Huang Xiaodan Sun Chaozong Liu |
| author_facet | Ziyu Liu Chunjing Tao Shanshan Yuan Wei Wang Maryam Tamaddon Liqi Ng Hao Huang Xiaodan Sun Chaozong Liu |
| author_sort | Ziyu Liu |
| collection | DOAJ |
| description | In tissue engineering field, it is important to develop a suitable numerical model to evaluate scaffold geometry design. The experimental evaluation of the effect of each specific scaffold parameter on tissue regeneration requires large cost and long time expend. Dynamic cell culture is commonly used for generating tissues which could replace damaged tissues. A perfusion bioreactor model is developed which is able to simulate dynamic cell culture, to evaluate scaffold quality. The wall-film model is used to simulate cell attachment with the assumption that cells could be seen as liquid drops. In the process of cell attachment, the cells could impinge to a solid surface and form a liquid film which were considered as cell attached on the scaffold surface. Two types of cell-scaffold interactions were involved in numerical models including trap model and Stanton-Rutland (Cell impinge model—CIM) model. For trap model, all cells impinged the scaffold are seen as attached. For Stanton-Rutland model, four regimes of cell-scaffold interaction are involved in the cell attachment, including stick, rebound, spread, and splash, and only stick and spread are seen as attached. By comparison with two different numerical methods, the results showed that CIM model result is more related to the experimental results than trap model, which indicated that four regimes of cell-scaffold interaction occurred in cell attachment process. By evaluating two different geometry scaffold's cells seeding by these two models, the results further indicated that this model are able to use for assessing the scaffold design. |
| first_indexed | 2024-12-13T19:54:21Z |
| format | Article |
| id | doaj.art-b35f813967344db4b4dfa104210432cf |
| institution | Directory Open Access Journal |
| issn | 2590-0935 |
| language | English |
| last_indexed | 2024-12-13T19:54:21Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Novel Technology and Devices |
| spelling | doaj.art-b35f813967344db4b4dfa104210432cf2022-12-21T23:33:21ZengElsevierMedicine in Novel Technology and Devices2590-09352022-03-0113100104Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactorZiyu Liu0Chunjing Tao1Shanshan Yuan2Wei Wang3Maryam Tamaddon4Liqi Ng5Hao Huang6Xiaodan Sun7Chaozong Liu8Division of Surgery & Interventional Science, University College London, Royal National Orthopaedic Hospital, Stanmore HA7 4LP, UK; School of Medical Science and Engineering, Beihang University, China; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing, ChinaSchool of Medical Science and Engineering, Beihang University, ChinaSchool of Medical Science and Engineering, Beihang University, China; Department of Cardiology, Qingdao Municipal Hospital, Shandong, ChinaSchool of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, ChinaDivision of Surgery & Interventional Science, University College London, Royal National Orthopaedic Hospital, Stanmore HA7 4LP, UKDivision of Surgery & Interventional Science, University College London, Royal National Orthopaedic Hospital, Stanmore HA7 4LP, UKSchool of Medical Science and Engineering, Beihang University, ChinaKey Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing, China; Corresponding author.Division of Surgery & Interventional Science, University College London, Royal National Orthopaedic Hospital, Stanmore HA7 4LP, UK; Corresponding author.In tissue engineering field, it is important to develop a suitable numerical model to evaluate scaffold geometry design. The experimental evaluation of the effect of each specific scaffold parameter on tissue regeneration requires large cost and long time expend. Dynamic cell culture is commonly used for generating tissues which could replace damaged tissues. A perfusion bioreactor model is developed which is able to simulate dynamic cell culture, to evaluate scaffold quality. The wall-film model is used to simulate cell attachment with the assumption that cells could be seen as liquid drops. In the process of cell attachment, the cells could impinge to a solid surface and form a liquid film which were considered as cell attached on the scaffold surface. Two types of cell-scaffold interactions were involved in numerical models including trap model and Stanton-Rutland (Cell impinge model—CIM) model. For trap model, all cells impinged the scaffold are seen as attached. For Stanton-Rutland model, four regimes of cell-scaffold interaction are involved in the cell attachment, including stick, rebound, spread, and splash, and only stick and spread are seen as attached. By comparison with two different numerical methods, the results showed that CIM model result is more related to the experimental results than trap model, which indicated that four regimes of cell-scaffold interaction occurred in cell attachment process. By evaluating two different geometry scaffold's cells seeding by these two models, the results further indicated that this model are able to use for assessing the scaffold design.http://www.sciencedirect.com/science/article/pii/S2590093521000485Scaffold structureComputational fluid dynamicsCell attachmentCell impinge model |
| spellingShingle | Ziyu Liu Chunjing Tao Shanshan Yuan Wei Wang Maryam Tamaddon Liqi Ng Hao Huang Xiaodan Sun Chaozong Liu Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor Medicine in Novel Technology and Devices Scaffold structure Computational fluid dynamics Cell attachment Cell impinge model |
| title | Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor |
| title_full | Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor |
| title_fullStr | Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor |
| title_full_unstemmed | Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor |
| title_short | Eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor |
| title_sort | eularian wall film model for predicting dynamic cell culture process to evaluate scaffold design in a perfusion bioreactor |
| topic | Scaffold structure Computational fluid dynamics Cell attachment Cell impinge model |
| url | http://www.sciencedirect.com/science/article/pii/S2590093521000485 |
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