Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration
Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and compu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-04-01
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| Series: | Bioengineering |
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| Online Access: | http://www.mdpi.com/2306-5354/5/2/33 |
| _version_ | 1797760027108311040 |
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| author | Tannaz Tajsoleiman Mohammad Jafar Abdekhodaie Krist V. Gernaey Ulrich Krühne |
| author_facet | Tannaz Tajsoleiman Mohammad Jafar Abdekhodaie Krist V. Gernaey Ulrich Krühne |
| author_sort | Tannaz Tajsoleiman |
| collection | DOAJ |
| description | Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs. Thereby, the exploitation of the predictive simulation into a newly developed stochastic routine provides the opportunity of exploring improved scaffold geometry designs. This approach was applied on a common type of fibrous structure in order to increase the process efficiencies compared with the regular used formats. The suggested topology supplies a larger effective surface for cell attachment compared to the reference design while the level of shear stress is kept at the positive range of effect. Moreover, significant improvement of mass transfer is predicted for the suggested topology. |
| first_indexed | 2024-03-12T18:52:51Z |
| format | Article |
| id | doaj.art-b360e732b90c48ebba3f6e30baa8d56f |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T18:52:51Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-b360e732b90c48ebba3f6e30baa8d56f2023-08-02T07:10:27ZengMDPI AGBioengineering2306-53542018-04-01523310.3390/bioengineering5020033bioengineering5020033Efficient Computational Design of a Scaffold for Cartilage Cell RegenerationTannaz Tajsoleiman0Mohammad Jafar Abdekhodaie1Krist V. Gernaey2Ulrich Krühne3Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs., Lyngby, DenmarkDepartment of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, IranDepartment of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs., Lyngby, DenmarkDepartment of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs., Lyngby, DenmarkDue to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs. Thereby, the exploitation of the predictive simulation into a newly developed stochastic routine provides the opportunity of exploring improved scaffold geometry designs. This approach was applied on a common type of fibrous structure in order to increase the process efficiencies compared with the regular used formats. The suggested topology supplies a larger effective surface for cell attachment compared to the reference design while the level of shear stress is kept at the positive range of effect. Moreover, significant improvement of mass transfer is predicted for the suggested topology.http://www.mdpi.com/2306-5354/5/2/33tissue engineeringCFD simulationscaffold geometry optimizationmicro-bioreactor operating conditions |
| spellingShingle | Tannaz Tajsoleiman Mohammad Jafar Abdekhodaie Krist V. Gernaey Ulrich Krühne Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration Bioengineering tissue engineering CFD simulation scaffold geometry optimization micro-bioreactor operating conditions |
| title | Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration |
| title_full | Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration |
| title_fullStr | Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration |
| title_full_unstemmed | Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration |
| title_short | Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration |
| title_sort | efficient computational design of a scaffold for cartilage cell regeneration |
| topic | tissue engineering CFD simulation scaffold geometry optimization micro-bioreactor operating conditions |
| url | http://www.mdpi.com/2306-5354/5/2/33 |
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