Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�

Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group) �Kluai Sa� and �Kluai Leb Mu Nang� on a modified Murashige and Skoog (MS) medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7...

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Main Authors: Kamnoon KANCHANAPOOM, Korn KORAPATCHAIKUL
Format: Article
Language:English
Published: Society of Land Measurements and Cadastre from Transylvania (SMTCT) 2012-02-01
Series:Notulae Scientia Biologicae
Online Access:http://notulaebiologicae.ro/index.php/nsb/article/view/7223
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author Kamnoon KANCHANAPOOM
Korn KORAPATCHAIKUL
author_facet Kamnoon KANCHANAPOOM
Korn KORAPATCHAIKUL
author_sort Kamnoon KANCHANAPOOM
collection DOAJ
description Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group) �Kluai Sa� and �Kluai Leb Mu Nang� on a modified Murashige and Skoog (MS) medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration was achieved through organogenesis in callus cultures. Regenerated shoots were rooted on MS medium containing 0.2% activated charcoal but without plant growth regulators. Histological analysis revealed that calluses originated from small dense cells with well stained cytoplasm and nucleus typical of meristematic cells.
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spelling doaj.art-b3613311e1704fd39cee989ba4eb4d4f2022-12-22T00:53:13ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642012-02-014194976917Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�Kamnoon KANCHANAPOOM0Korn KORAPATCHAIKUL1Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla, 90112Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla, 90112Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group) �Kluai Sa� and �Kluai Leb Mu Nang� on a modified Murashige and Skoog (MS) medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration was achieved through organogenesis in callus cultures. Regenerated shoots were rooted on MS medium containing 0.2% activated charcoal but without plant growth regulators. Histological analysis revealed that calluses originated from small dense cells with well stained cytoplasm and nucleus typical of meristematic cells.http://notulaebiologicae.ro/index.php/nsb/article/view/7223
spellingShingle Kamnoon KANCHANAPOOM
Korn KORAPATCHAIKUL
Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�
Notulae Scientia Biologicae
title Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�
title_full Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�
title_fullStr Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�
title_full_unstemmed Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�
title_short Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�
title_sort histology of callogenesis in diploid bananas musa acuminata aa group �kluai sa� and �kluai leb mu nang�
url http://notulaebiologicae.ro/index.php/nsb/article/view/7223
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