Inhibition of Orbivirus Replication by Aurintricarboxylic Acid
Bluetongue virus (BTV) and African horse sickness virus (AHSV) are vector-borne viruses belonging to the <i>Orbivirus</i> genus, which are transmitted between hosts primarily by biting midges of the genus <i>Culicoides</i>. With recent BTV and AHSV outbreaks causing epidemics...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/19/7294 |
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| author | Celia Alonso Sergio Utrilla-Trigo Eva Calvo-Pinilla Luis Jiménez-Cabello Javier Ortego Aitor Nogales |
| author_facet | Celia Alonso Sergio Utrilla-Trigo Eva Calvo-Pinilla Luis Jiménez-Cabello Javier Ortego Aitor Nogales |
| author_sort | Celia Alonso |
| collection | DOAJ |
| description | Bluetongue virus (BTV) and African horse sickness virus (AHSV) are vector-borne viruses belonging to the <i>Orbivirus</i> genus, which are transmitted between hosts primarily by biting midges of the genus <i>Culicoides</i>. With recent BTV and AHSV outbreaks causing epidemics and important economy losses, there is a pressing need for efficacious drugs to treat and control the spread of these infections. The polyanionic aromatic compound aurintricarboxylic acid (ATA) has been shown to have a broad-spectrum antiviral activity. Here, we evaluated ATA as a potential antiviral compound against <i>Orbivirus</i> infections in both mammalian and insect cells. Notably, ATA was able to prevent the replication of BTV and AHSV in both cell types in a time- and concentration-dependent manner. In addition, we evaluated the effect of ATA in vivo using a mouse model of infection. ATA did not protect mice against a lethal challenge with BTV or AHSV, most probably due to the in vivo effect of ATA on immune system regulation. Overall, these results demonstrate that ATA has inhibitory activity against <i>Orbivirus</i> replication in vitro, but further in vivo analysis will be required before considering it as a potential therapy for future clinical evaluation. |
| first_indexed | 2024-03-10T15:53:12Z |
| format | Article |
| id | doaj.art-b3616d00c4dc4b7a9a8b6e61ff37e2bf |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:53:12Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b3616d00c4dc4b7a9a8b6e61ff37e2bf2023-11-20T15:56:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012119729410.3390/ijms21197294Inhibition of Orbivirus Replication by Aurintricarboxylic AcidCelia Alonso0Sergio Utrilla-Trigo1Eva Calvo-Pinilla2Luis Jiménez-Cabello3Javier Ortego4Aitor Nogales5Animal Health Research Centre (CISA), National Institute for Agriculture and Food Research and Technology (INIA), Valdeolmos, 28130 Madrid, SpainAnimal Health Research Centre (CISA), National Institute for Agriculture and Food Research and Technology (INIA), Valdeolmos, 28130 Madrid, SpainAnimal Health Research Centre (CISA), National Institute for Agriculture and Food Research and Technology (INIA), Valdeolmos, 28130 Madrid, SpainAnimal Health Research Centre (CISA), National Institute for Agriculture and Food Research and Technology (INIA), Valdeolmos, 28130 Madrid, SpainAnimal Health Research Centre (CISA), National Institute for Agriculture and Food Research and Technology (INIA), Valdeolmos, 28130 Madrid, SpainAnimal Health Research Centre (CISA), National Institute for Agriculture and Food Research and Technology (INIA), Valdeolmos, 28130 Madrid, SpainBluetongue virus (BTV) and African horse sickness virus (AHSV) are vector-borne viruses belonging to the <i>Orbivirus</i> genus, which are transmitted between hosts primarily by biting midges of the genus <i>Culicoides</i>. With recent BTV and AHSV outbreaks causing epidemics and important economy losses, there is a pressing need for efficacious drugs to treat and control the spread of these infections. The polyanionic aromatic compound aurintricarboxylic acid (ATA) has been shown to have a broad-spectrum antiviral activity. Here, we evaluated ATA as a potential antiviral compound against <i>Orbivirus</i> infections in both mammalian and insect cells. Notably, ATA was able to prevent the replication of BTV and AHSV in both cell types in a time- and concentration-dependent manner. In addition, we evaluated the effect of ATA in vivo using a mouse model of infection. ATA did not protect mice against a lethal challenge with BTV or AHSV, most probably due to the in vivo effect of ATA on immune system regulation. Overall, these results demonstrate that ATA has inhibitory activity against <i>Orbivirus</i> replication in vitro, but further in vivo analysis will be required before considering it as a potential therapy for future clinical evaluation.https://www.mdpi.com/1422-0067/21/19/7294<i>Orbivirus</i>ArbovirusBluetongue virusAfrican horse sickness virusaurintricarboxylic acidantiviral |
| spellingShingle | Celia Alonso Sergio Utrilla-Trigo Eva Calvo-Pinilla Luis Jiménez-Cabello Javier Ortego Aitor Nogales Inhibition of Orbivirus Replication by Aurintricarboxylic Acid International Journal of Molecular Sciences <i>Orbivirus</i> Arbovirus Bluetongue virus African horse sickness virus aurintricarboxylic acid antiviral |
| title | Inhibition of Orbivirus Replication by Aurintricarboxylic Acid |
| title_full | Inhibition of Orbivirus Replication by Aurintricarboxylic Acid |
| title_fullStr | Inhibition of Orbivirus Replication by Aurintricarboxylic Acid |
| title_full_unstemmed | Inhibition of Orbivirus Replication by Aurintricarboxylic Acid |
| title_short | Inhibition of Orbivirus Replication by Aurintricarboxylic Acid |
| title_sort | inhibition of orbivirus replication by aurintricarboxylic acid |
| topic | <i>Orbivirus</i> Arbovirus Bluetongue virus African horse sickness virus aurintricarboxylic acid antiviral |
| url | https://www.mdpi.com/1422-0067/21/19/7294 |
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