Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage Cell
<p><strong>Background:</strong><em> </em><em>Acanthamoeba keratitis</em> (AK) is potentially a sight-threatening infection and its treatment is challenging. This is mainly due to presence of resistant cyst form. Indeed, cysts are highly resistant to current...
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| Format: | Article |
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Shahid Beheshti University of Medical Sciences
2015-10-01
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| Series: | Novelty in Biomedicine |
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| Online Access: | http://journals.sbmu.ac.ir/index.php/nbm/article/view/8799 |
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| author | Samira Dodangeh Maryam Niyyati Mohammad Kamalinejad |
| author_facet | Samira Dodangeh Maryam Niyyati Mohammad Kamalinejad |
| author_sort | Samira Dodangeh |
| collection | DOAJ |
| description | <p><strong>Background:</strong><em> </em><em>Acanthamoeba keratitis</em> (AK) is potentially a sight-threatening infection and its treatment is challenging. This is mainly due to presence of resistant cyst form. Indeed, cysts are highly resistant to current available drugs. Chemical drugs are toxic to human keratocytes. It should also be mentioned that most available anti-<em>Acanthamoeba</em> drugs are poorly cysticidal, In Iran and worldwide, AK cases continue to rise and therefore, novel effective drugs are urgently needed for the treatment of AK.</p><p><strong>M</strong><strong>aterials and Methods: </strong>In the present study, the <em>in </em><em>vitro </em>activity of serial dilutions (10, 15, 20 and 25 mg/mL) of<em> </em>chloroformic fractions including primary<strong> </strong>chloroformic fraction (minimum amount of chloroform), middle chloroformic fraction and remaining chloroformic fraction (most amount of chloroform) of <em>Trigonella foenum graecum</em> seed were evaluated against <em>Acanthamoeba </em>trophozoites and cysts. Cytotoxic assay of fractions at different concentrations (25, 50, 100, 200, 300, 400, 500 mg/ml) of test material was identified on mice Macrophage cells using MTT method.</p><p><strong>R</strong><strong>es</strong><strong>ults: </strong>The obtained results revealed that the tested fractions presented anti-amoebic activities in a time and dose dependent cycle. Anti-<em>Acanthamoeba</em> activity of remaining chloroformic fraction was more than other fractions. Trophozoites/cysts were eliminated when incubated with 15 and 20 mg/ml concentrations of remaining chloroformic fraction after 24 hours. Viability of macrophage cells was noted 100 % with 25 and 50 mg/ml concentration of remaining chloroformic fraction. Our results indicate that the plant fractions are safe for mammalian cells.</p><p><strong>Conclusion</strong><strong>: </strong>Further studies should be performed in order to detect the active chemical compounds which could be used for the development of novel therapeutic approaches against <em>Acanthamoeba</em> infections. To the best of our knowledge, this is the first study on the activity of chloroformic fractions of <em>Trigonella foenum graecum</em> (seed) against <em>Acanthamoeba</em> spp<em>.</em></p> |
| first_indexed | 2024-12-10T08:41:57Z |
| format | Article |
| id | doaj.art-b36455e07d5149ce91ae354381951bb6 |
| institution | Directory Open Access Journal |
| issn | 2345-3346 2345-3907 |
| language | English |
| last_indexed | 2024-12-10T08:41:57Z |
| publishDate | 2015-10-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Novelty in Biomedicine |
| spelling | doaj.art-b36455e07d5149ce91ae354381951bb62022-12-22T01:55:49ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-33462345-39072015-10-01341821885784Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage CellSamira Dodangeh0Maryam Niyyati1Mohammad Kamalinejad2Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranSchool of Pharmacology, Shahid Beheshti University of Medical Sciences, Tehran, Iran<p><strong>Background:</strong><em> </em><em>Acanthamoeba keratitis</em> (AK) is potentially a sight-threatening infection and its treatment is challenging. This is mainly due to presence of resistant cyst form. Indeed, cysts are highly resistant to current available drugs. Chemical drugs are toxic to human keratocytes. It should also be mentioned that most available anti-<em>Acanthamoeba</em> drugs are poorly cysticidal, In Iran and worldwide, AK cases continue to rise and therefore, novel effective drugs are urgently needed for the treatment of AK.</p><p><strong>M</strong><strong>aterials and Methods: </strong>In the present study, the <em>in </em><em>vitro </em>activity of serial dilutions (10, 15, 20 and 25 mg/mL) of<em> </em>chloroformic fractions including primary<strong> </strong>chloroformic fraction (minimum amount of chloroform), middle chloroformic fraction and remaining chloroformic fraction (most amount of chloroform) of <em>Trigonella foenum graecum</em> seed were evaluated against <em>Acanthamoeba </em>trophozoites and cysts. Cytotoxic assay of fractions at different concentrations (25, 50, 100, 200, 300, 400, 500 mg/ml) of test material was identified on mice Macrophage cells using MTT method.</p><p><strong>R</strong><strong>es</strong><strong>ults: </strong>The obtained results revealed that the tested fractions presented anti-amoebic activities in a time and dose dependent cycle. Anti-<em>Acanthamoeba</em> activity of remaining chloroformic fraction was more than other fractions. Trophozoites/cysts were eliminated when incubated with 15 and 20 mg/ml concentrations of remaining chloroformic fraction after 24 hours. Viability of macrophage cells was noted 100 % with 25 and 50 mg/ml concentration of remaining chloroformic fraction. Our results indicate that the plant fractions are safe for mammalian cells.</p><p><strong>Conclusion</strong><strong>: </strong>Further studies should be performed in order to detect the active chemical compounds which could be used for the development of novel therapeutic approaches against <em>Acanthamoeba</em> infections. To the best of our knowledge, this is the first study on the activity of chloroformic fractions of <em>Trigonella foenum graecum</em> (seed) against <em>Acanthamoeba</em> spp<em>.</em></p>http://journals.sbmu.ac.ir/index.php/nbm/article/view/8799Acanthamoeba, chloroformic fractions, In vitro, Therapy |
| spellingShingle | Samira Dodangeh Maryam Niyyati Mohammad Kamalinejad Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage Cell Novelty in Biomedicine Acanthamoeba, chloroformic fractions, In vitro, Therapy |
| title | Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage Cell |
| title_full | Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage Cell |
| title_fullStr | Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage Cell |
| title_full_unstemmed | Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage Cell |
| title_short | Anti-Acanthamoeba Activities of Chloroformic Fractions of Trigonella Foenum Graecum (Seed) and Their Cytotoxity on Mice Macrophage Cell |
| title_sort | anti acanthamoeba activities of chloroformic fractions of trigonella foenum graecum seed and their cytotoxity on mice macrophage cell |
| topic | Acanthamoeba, chloroformic fractions, In vitro, Therapy |
| url | http://journals.sbmu.ac.ir/index.php/nbm/article/view/8799 |
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