Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize
Genetically modified (GM) maize is one of the earliest GM crops to have achieved large-scale commercial cultivation globally, and it is of great significance to excel in the development and implementation of safety policy regarding GM, and in its technical oversight. This article describes the gener...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12247 |
| _version_ | 1797586623870795776 |
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| author | Tongyun Luo Lujing Li Shirui Wang Nan Cheng |
| author_facet | Tongyun Luo Lujing Li Shirui Wang Nan Cheng |
| author_sort | Tongyun Luo |
| collection | DOAJ |
| description | Genetically modified (GM) maize is one of the earliest GM crops to have achieved large-scale commercial cultivation globally, and it is of great significance to excel in the development and implementation of safety policy regarding GM, and in its technical oversight. This article describes the general situation regarding genetically modified maize, including its varieties, applications, relevant laws and regulations, and so on. From a technical point of view, we summarize and critically analyze the existing methods for detecting nucleic acid levels in genetically modified maize. The nucleic acid extraction technology used for maize is explained, and the introduction of traditional detection techniques, which cover variable-temperature and isothermal amplification detection technology and gene chip technology, applications in maize are described. Moreover, new technologies are proposed, with special attention paid to nucleic acid detection methods using sensors. Finally, we review the current limitations and challenges of GM maize nucleic acid testing and share our vision for the future direction of this field. |
| first_indexed | 2024-03-11T00:25:43Z |
| format | Article |
| id | doaj.art-b367cc5cce7d4d33b58666fdcad24f9c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:25:43Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b367cc5cce7d4d33b58666fdcad24f9c2023-11-18T23:02:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124151224710.3390/ijms241512247Research Progress of Nucleic Acid Detection Technology for Genetically Modified MaizeTongyun Luo0Lujing Li1Shirui Wang2Nan Cheng3College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaGenetically modified (GM) maize is one of the earliest GM crops to have achieved large-scale commercial cultivation globally, and it is of great significance to excel in the development and implementation of safety policy regarding GM, and in its technical oversight. This article describes the general situation regarding genetically modified maize, including its varieties, applications, relevant laws and regulations, and so on. From a technical point of view, we summarize and critically analyze the existing methods for detecting nucleic acid levels in genetically modified maize. The nucleic acid extraction technology used for maize is explained, and the introduction of traditional detection techniques, which cover variable-temperature and isothermal amplification detection technology and gene chip technology, applications in maize are described. Moreover, new technologies are proposed, with special attention paid to nucleic acid detection methods using sensors. Finally, we review the current limitations and challenges of GM maize nucleic acid testing and share our vision for the future direction of this field.https://www.mdpi.com/1422-0067/24/15/12247genetically modified maizeamplification technologynucleic acid detectionsensor |
| spellingShingle | Tongyun Luo Lujing Li Shirui Wang Nan Cheng Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize International Journal of Molecular Sciences genetically modified maize amplification technology nucleic acid detection sensor |
| title | Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize |
| title_full | Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize |
| title_fullStr | Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize |
| title_full_unstemmed | Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize |
| title_short | Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize |
| title_sort | research progress of nucleic acid detection technology for genetically modified maize |
| topic | genetically modified maize amplification technology nucleic acid detection sensor |
| url | https://www.mdpi.com/1422-0067/24/15/12247 |
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