Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation
Background: Gualou Xiebai Decoction (GLXB) is a classic prescription of Chinese medicine used for the treatment of cardiac problems. The present study was designed to explore the effect and mechanism of GLXB on ischemia/reperfusion (I/R) induced disorders in myocardial structure and function, focusi...
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00296/full |
| _version_ | 1818031211995463680 |
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| author | Lu-Lu Yan Lu-Lu Yan Wei-Yang Zhang Xiao-Hong Wei Xiao-Hong Wei Li Yan Chun-Shui Pan Yang Yu Jing-Yu Fan Yu-Ying Liu Yu-Ying Liu Hua Zhou Jing-Yan Han Jing-Yan Han Xin-Sheng Yao Xin-Sheng Yao |
| author_facet | Lu-Lu Yan Lu-Lu Yan Wei-Yang Zhang Xiao-Hong Wei Xiao-Hong Wei Li Yan Chun-Shui Pan Yang Yu Jing-Yu Fan Yu-Ying Liu Yu-Ying Liu Hua Zhou Jing-Yan Han Jing-Yan Han Xin-Sheng Yao Xin-Sheng Yao |
| author_sort | Lu-Lu Yan |
| collection | DOAJ |
| description | Background: Gualou Xiebai Decoction (GLXB) is a classic prescription of Chinese medicine used for the treatment of cardiac problems. The present study was designed to explore the effect and mechanism of GLXB on ischemia/reperfusion (I/R) induced disorders in myocardial structure and function, focusing on the regulation of energy metabolism and the RhoA/ROCK pathway.Methods: After hyperlipidemic rat model was established by oral administration of high fat diet, the rats were treated with GLXB for 6 weeks and subjected to 30 min occlusion of the left anterior descending coronary artery (LADCA) followed by 90 min reperfusion to elicit I/R challenge. Myocardial infarct size was assessed by Evans blue-TTC staining. Myocardial blood flow (MBF) and cardiac function were evaluated. Enzyme-linked immunosorbent assay was performed to examine the content of ATP, ADP, AMP, CK, CK-MB, LDH, cTnT, cTnI, and IL-6. Double staining of F-actin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. Expressions of ATP synthase subunit δ (ATP 5D), and RhoA and ROCK were determined by Western blotting.Results: Administration with GLXB at high dose for 6 weeks protected heart against I/R-induced MBF decrease, myocardial infarction and apoptosis, ameliorated I/R-caused impairment of cardiac function and myocardial structure, restored the decrease in the ratio of ADP/ATP and AMP/ATP, and the expression of ATP 5D with inhibiting the expression of RhoA and ROCK.Conclusions: Treatment with GLXB effectively protects myocardial structure and function from I/R challenge, possibly via regulating energy metabolism involving inactivation of RhoA/ROCK signaling pathway. |
| first_indexed | 2024-12-10T05:47:53Z |
| format | Article |
| id | doaj.art-b36ab70799bc48948a7dc23b1405ac35 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T05:47:53Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-b36ab70799bc48948a7dc23b1405ac352022-12-22T02:00:06ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-04-01910.3389/fphys.2018.00296338992Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy ModulationLu-Lu Yan0Lu-Lu Yan1Wei-Yang Zhang2Xiao-Hong Wei3Xiao-Hong Wei4Li Yan5Chun-Shui Pan6Yang Yu7Jing-Yu Fan8Yu-Ying Liu9Yu-Ying Liu10Hua Zhou11Jing-Yan Han12Jing-Yan Han13Xin-Sheng Yao14Xin-Sheng Yao15State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaDepartment of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaInstitute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, ChinaDepartment of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaDepartment of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaDepartment of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, ChinaInstitute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, ChinaBackground: Gualou Xiebai Decoction (GLXB) is a classic prescription of Chinese medicine used for the treatment of cardiac problems. The present study was designed to explore the effect and mechanism of GLXB on ischemia/reperfusion (I/R) induced disorders in myocardial structure and function, focusing on the regulation of energy metabolism and the RhoA/ROCK pathway.Methods: After hyperlipidemic rat model was established by oral administration of high fat diet, the rats were treated with GLXB for 6 weeks and subjected to 30 min occlusion of the left anterior descending coronary artery (LADCA) followed by 90 min reperfusion to elicit I/R challenge. Myocardial infarct size was assessed by Evans blue-TTC staining. Myocardial blood flow (MBF) and cardiac function were evaluated. Enzyme-linked immunosorbent assay was performed to examine the content of ATP, ADP, AMP, CK, CK-MB, LDH, cTnT, cTnI, and IL-6. Double staining of F-actin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. Expressions of ATP synthase subunit δ (ATP 5D), and RhoA and ROCK were determined by Western blotting.Results: Administration with GLXB at high dose for 6 weeks protected heart against I/R-induced MBF decrease, myocardial infarction and apoptosis, ameliorated I/R-caused impairment of cardiac function and myocardial structure, restored the decrease in the ratio of ADP/ATP and AMP/ATP, and the expression of ATP 5D with inhibiting the expression of RhoA and ROCK.Conclusions: Treatment with GLXB effectively protects myocardial structure and function from I/R challenge, possibly via regulating energy metabolism involving inactivation of RhoA/ROCK signaling pathway.http://journal.frontiersin.org/article/10.3389/fphys.2018.00296/fullcardiac functionmyocardial structureATP synthase subunitapoptosisRho-associated protein kinaseRho GTPases |
| spellingShingle | Lu-Lu Yan Lu-Lu Yan Wei-Yang Zhang Xiao-Hong Wei Xiao-Hong Wei Li Yan Chun-Shui Pan Yang Yu Jing-Yu Fan Yu-Ying Liu Yu-Ying Liu Hua Zhou Jing-Yan Han Jing-Yan Han Xin-Sheng Yao Xin-Sheng Yao Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation Frontiers in Physiology cardiac function myocardial structure ATP synthase subunit apoptosis Rho-associated protein kinase Rho GTPases |
| title | Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation |
| title_full | Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation |
| title_fullStr | Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation |
| title_full_unstemmed | Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation |
| title_short | Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation |
| title_sort | gualou xiebai decoction a traditional chinese medicine prevents cardiac reperfusion injury of hyperlipidemia rat via energy modulation |
| topic | cardiac function myocardial structure ATP synthase subunit apoptosis Rho-associated protein kinase Rho GTPases |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2018.00296/full |
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