A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System
The ability of some bacteria to perform Extracellular Electron Transfer (EET) has been explored in bioelectrochemical systems (BES) to obtain energy or chemicals from pure substances or residual substrates. Here, a new pyoverdine-producing <i>Pseudomonas aeruginosa</i> strain was isolate...
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MDPI AG
2023-07-01
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| author | Julia Pereira Narcizo Lucca Bonjy Kikuti Mancilio Matheus Pedrino María-Eugenia Guazzaroni Adalgisa Rodrigues de Andrade Valeria Reginatto |
| author_facet | Julia Pereira Narcizo Lucca Bonjy Kikuti Mancilio Matheus Pedrino María-Eugenia Guazzaroni Adalgisa Rodrigues de Andrade Valeria Reginatto |
| author_sort | Julia Pereira Narcizo |
| collection | DOAJ |
| description | The ability of some bacteria to perform Extracellular Electron Transfer (EET) has been explored in bioelectrochemical systems (BES) to obtain energy or chemicals from pure substances or residual substrates. Here, a new pyoverdine-producing <i>Pseudomonas aeruginosa</i> strain was isolated from an MFC biofilm oxidizing glycerol, a by-product of biodiesel production. Strain EL14 was investigated to assess its electrogenic ability and products. In an open circuit system (fermentation system), EL14 was able to consume glycerol and produce 1,3-propanediol, an unusual product from glycerol oxidation in <i>P. aeruginosa</i>. The microbial fuel cell (MFC) EL14 reached a current density of 82.4 mA m<sup>−2</sup> during the first feeding cycle, then dropped sharply as the biofilm fell off. Cyclic voltammetry suggests that electron transfer to the anode occurs indirectly, i.e., through a redox substance, with redox peak at 0.22 V (vs Ag/AgCl), and directly probably by membrane redox proteins, with redox peak at 0.05 V (vs Ag/AgCl). EL14 produced added-value bioproducts, acetic and butyric acids, as well as 1,3 propanediol, in both fermentative and anodic conditions. However, the yield of 1,3-PDO from glycerol was enhanced from 0.57 to 0.89 (mol of 1,3-PDO mol<sup>−1</sup> of glycerol) under MFC conditions compared to fermentation. This result was unexpected, since successful 1,3-PDO production is not usually associated with <i>P. aeruginosa</i> glycerol metabolism. By comparing EL14 genomic sequences related to the 1,3-PDO biosynthesis with <i>P. aeruginosa</i> reference strains, we observed that strain EL14 has three copies of the <i>dhaT</i> gene (1,3-propanediol dehydrogenase a different arrangement compared to other <i>Pseudomonas</i> isolates). Thus, this work functionally characterizes a bacterium never before associated with 1,3-PDO biosynthesis, indicating its potential for converting a by-product of the biodiesel industry into an emerging chemical product. |
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| spelling | doaj.art-b36b9f610e0f479085df19e0c3d03a592023-11-18T18:45:15ZengMDPI AGCatalysts2073-43442023-07-01137113310.3390/catal13071133A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical SystemJulia Pereira Narcizo0Lucca Bonjy Kikuti Mancilio1Matheus Pedrino2María-Eugenia Guazzaroni3Adalgisa Rodrigues de Andrade4Valeria Reginatto5Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto CEP 14040-901, SP, BrazilDepartment of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto CEP 14040-901, SP, BrazilDepartment of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto CEP 14040-901, SP, BrazilDepartment of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto CEP 14040-901, SP, BrazilDepartment of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto CEP 14040-901, SP, BrazilDepartment of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto CEP 14040-901, SP, BrazilThe ability of some bacteria to perform Extracellular Electron Transfer (EET) has been explored in bioelectrochemical systems (BES) to obtain energy or chemicals from pure substances or residual substrates. Here, a new pyoverdine-producing <i>Pseudomonas aeruginosa</i> strain was isolated from an MFC biofilm oxidizing glycerol, a by-product of biodiesel production. Strain EL14 was investigated to assess its electrogenic ability and products. In an open circuit system (fermentation system), EL14 was able to consume glycerol and produce 1,3-propanediol, an unusual product from glycerol oxidation in <i>P. aeruginosa</i>. The microbial fuel cell (MFC) EL14 reached a current density of 82.4 mA m<sup>−2</sup> during the first feeding cycle, then dropped sharply as the biofilm fell off. Cyclic voltammetry suggests that electron transfer to the anode occurs indirectly, i.e., through a redox substance, with redox peak at 0.22 V (vs Ag/AgCl), and directly probably by membrane redox proteins, with redox peak at 0.05 V (vs Ag/AgCl). EL14 produced added-value bioproducts, acetic and butyric acids, as well as 1,3 propanediol, in both fermentative and anodic conditions. However, the yield of 1,3-PDO from glycerol was enhanced from 0.57 to 0.89 (mol of 1,3-PDO mol<sup>−1</sup> of glycerol) under MFC conditions compared to fermentation. This result was unexpected, since successful 1,3-PDO production is not usually associated with <i>P. aeruginosa</i> glycerol metabolism. By comparing EL14 genomic sequences related to the 1,3-PDO biosynthesis with <i>P. aeruginosa</i> reference strains, we observed that strain EL14 has three copies of the <i>dhaT</i> gene (1,3-propanediol dehydrogenase a different arrangement compared to other <i>Pseudomonas</i> isolates). Thus, this work functionally characterizes a bacterium never before associated with 1,3-PDO biosynthesis, indicating its potential for converting a by-product of the biodiesel industry into an emerging chemical product.https://www.mdpi.com/2073-4344/13/7/1133<i>Pseudomonas aeruginosa</i>bioelectrochemical systemsfermentation1,3-propanediolmicrobial fuel cells |
| spellingShingle | Julia Pereira Narcizo Lucca Bonjy Kikuti Mancilio Matheus Pedrino María-Eugenia Guazzaroni Adalgisa Rodrigues de Andrade Valeria Reginatto A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System Catalysts <i>Pseudomonas aeruginosa</i> bioelectrochemical systems fermentation 1,3-propanediol microbial fuel cells |
| title | A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System |
| title_full | A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System |
| title_fullStr | A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System |
| title_full_unstemmed | A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System |
| title_short | A New <i>Pseudomonas aeruginosa</i> Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System |
| title_sort | new i pseudomonas aeruginosa i isolate enhances its unusual 1 3 propanediol generation from glycerol in bioelectrochemical system |
| topic | <i>Pseudomonas aeruginosa</i> bioelectrochemical systems fermentation 1,3-propanediol microbial fuel cells |
| url | https://www.mdpi.com/2073-4344/13/7/1133 |
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