Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic Checkpoints
Transcription factor NRF2 is a master regulator of the multiple cytoprotective responses that confer growth advantages on a cell. However, its participation in the mechanisms that govern the cell division cycle has not been explored in detail. In this study, we used several standard methods of synch...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/946 |
| _version_ | 1797501955810000896 |
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| author | Diego Lastra Maribel Escoll Antonio Cuadrado |
| author_facet | Diego Lastra Maribel Escoll Antonio Cuadrado |
| author_sort | Diego Lastra |
| collection | DOAJ |
| description | Transcription factor NRF2 is a master regulator of the multiple cytoprotective responses that confer growth advantages on a cell. However, its participation in the mechanisms that govern the cell division cycle has not been explored in detail. In this study, we used several standard methods of synchronization of proliferating cells together with flow cytometry and monitored the participation of NRF2 along the cell cycle by the knockdown of its gene expression. We found that the NRF2 levels were highest at S phase entry, and lowest at mitosis. NRF2 depletion promoted both G1 and M arrest. Targeted transcriptomics analysis of cell cycle regulators showed that NRF2 depletion leads to changes in key cell cycle regulators, such as <i>CDK2</i>, <i>TFDP1</i>, <i>CDK6</i>, <i>CDKN1A</i> (p21), <i>CDKN1B</i> (p27), <i>CCNG1</i>, and <i>RAD51</i>. This study gives a new dimension to NRF2 effects, showing their implication in cell cycle progression. |
| first_indexed | 2024-03-10T03:26:38Z |
| format | Article |
| id | doaj.art-b36bf06ad32c48f0813244fabcef7b47 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:38Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b36bf06ad32c48f0813244fabcef7b472023-11-23T09:52:08ZengMDPI AGAntioxidants2076-39212022-05-0111594610.3390/antiox11050946Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic CheckpointsDiego Lastra0Maribel Escoll1Antonio Cuadrado2Department of Biochemistry, Medical College, Autonomous University of Madrid (UAM), 28029 Madrid, SpainDepartment of Biochemistry, Medical College, Autonomous University of Madrid (UAM), 28029 Madrid, SpainDepartment of Biochemistry, Medical College, Autonomous University of Madrid (UAM), 28029 Madrid, SpainTranscription factor NRF2 is a master regulator of the multiple cytoprotective responses that confer growth advantages on a cell. However, its participation in the mechanisms that govern the cell division cycle has not been explored in detail. In this study, we used several standard methods of synchronization of proliferating cells together with flow cytometry and monitored the participation of NRF2 along the cell cycle by the knockdown of its gene expression. We found that the NRF2 levels were highest at S phase entry, and lowest at mitosis. NRF2 depletion promoted both G1 and M arrest. Targeted transcriptomics analysis of cell cycle regulators showed that NRF2 depletion leads to changes in key cell cycle regulators, such as <i>CDK2</i>, <i>TFDP1</i>, <i>CDK6</i>, <i>CDKN1A</i> (p21), <i>CDKN1B</i> (p27), <i>CCNG1</i>, and <i>RAD51</i>. This study gives a new dimension to NRF2 effects, showing their implication in cell cycle progression.https://www.mdpi.com/2076-3921/11/5/946cell division cyclerestriction pointcheck pointNRF2 |
| spellingShingle | Diego Lastra Maribel Escoll Antonio Cuadrado Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic Checkpoints Antioxidants cell division cycle restriction point check point NRF2 |
| title | Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic Checkpoints |
| title_full | Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic Checkpoints |
| title_fullStr | Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic Checkpoints |
| title_full_unstemmed | Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic Checkpoints |
| title_short | Transcription Factor NRF2 Participates in Cell Cycle Progression at the Level of G1/S and Mitotic Checkpoints |
| title_sort | transcription factor nrf2 participates in cell cycle progression at the level of g1 s and mitotic checkpoints |
| topic | cell division cycle restriction point check point NRF2 |
| url | https://www.mdpi.com/2076-3921/11/5/946 |
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