Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process
Abstract Background Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the commercial value of sunflower processing industry, a two-step dilute sulfur...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-021-02045-2 |
| _version_ | 1817977395273007104 |
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| author | Qibo Zhang Lu Cheng Xutong Ma Xin Zhou Yong Xu |
| author_facet | Qibo Zhang Lu Cheng Xutong Ma Xin Zhou Yong Xu |
| author_sort | Qibo Zhang |
| collection | DOAJ |
| description | Abstract Background Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the commercial value of sunflower processing industry, a two-step dilute sulfuric acid treatment process was conducted on spent sunflower stalk pith to obtain the value-added products, pectin and glucose. Results In this study, pectin was firstly extracted under mild acid condition to avoid pectin degradation, which was conducted at 90 °C with a pH of 2.0 for 2 h, and ~0.14 g/g of pectin could be recovered. Then the remaining solids after pectin extraction were subjected to the reinforced treatment process with 0.75% H2SO4 at 150 °C for 30 min to further improve enzymatic hydrolysis efficiency. Moreover, by combining a fed-batch enzymatic hydrolysis strategy, a solid loading content of 16% was successfully achieved and the glucose titer reached 103.1 g/L with a yield of 83.6%. Conclusion Finally, ~140 g pectin and 260 g glucose were produced from 1 kg of raw sunflower stalk pith using the integrated biorefinery process. This work puts forward a two-step dilute acid pretreatment combined with enzymatic hydrolysis method to produce pectin and glucose from sunflower spent waste. |
| first_indexed | 2024-04-13T22:15:50Z |
| format | Article |
| id | doaj.art-b36f41090e094267a2a2b4838fffe5b1 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:15:50Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-b36f41090e094267a2a2b4838fffe5b12022-12-22T02:27:33ZengBMCBiotechnology for Biofuels1754-68342021-10-011411910.1186/s13068-021-02045-2Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment processQibo Zhang0Lu Cheng1Xutong Ma2Xin Zhou3Yong Xu4Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Products, Nanjing Forestry UniversityJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Products, Nanjing Forestry UniversityJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Products, Nanjing Forestry UniversityJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Products, Nanjing Forestry UniversityJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Products, Nanjing Forestry UniversityAbstract Background Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the commercial value of sunflower processing industry, a two-step dilute sulfuric acid treatment process was conducted on spent sunflower stalk pith to obtain the value-added products, pectin and glucose. Results In this study, pectin was firstly extracted under mild acid condition to avoid pectin degradation, which was conducted at 90 °C with a pH of 2.0 for 2 h, and ~0.14 g/g of pectin could be recovered. Then the remaining solids after pectin extraction were subjected to the reinforced treatment process with 0.75% H2SO4 at 150 °C for 30 min to further improve enzymatic hydrolysis efficiency. Moreover, by combining a fed-batch enzymatic hydrolysis strategy, a solid loading content of 16% was successfully achieved and the glucose titer reached 103.1 g/L with a yield of 83.6%. Conclusion Finally, ~140 g pectin and 260 g glucose were produced from 1 kg of raw sunflower stalk pith using the integrated biorefinery process. This work puts forward a two-step dilute acid pretreatment combined with enzymatic hydrolysis method to produce pectin and glucose from sunflower spent waste.https://doi.org/10.1186/s13068-021-02045-2Sunflower stalk pithTwo-step acid treatmentPectinGlucoseEnzymatic hydrolysis |
| spellingShingle | Qibo Zhang Lu Cheng Xutong Ma Xin Zhou Yong Xu Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process Biotechnology for Biofuels Sunflower stalk pith Two-step acid treatment Pectin Glucose Enzymatic hydrolysis |
| title | Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process |
| title_full | Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process |
| title_fullStr | Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process |
| title_full_unstemmed | Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process |
| title_short | Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process |
| title_sort | revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two step dilute acid pretreatment process |
| topic | Sunflower stalk pith Two-step acid treatment Pectin Glucose Enzymatic hydrolysis |
| url | https://doi.org/10.1186/s13068-021-02045-2 |
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