Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524
As previously demonstrated by our research group, the oral multicomponent drug Xraphconn<sup>®</sup> containing GS-441524 was effective at curing otherwise fatal feline infectious peritonitis (FIP) in 18 feline coronavirus (FCoV)-infected cats. The aims of the current study were to inves...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/5/1069 |
| _version_ | 1827665918418747392 |
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| author | Marina L. Meli Andrea M. Spiri Katharina Zwicklbauer Daniela Krentz Sandra Felten Michèle Bergmann Roswitha Dorsch Kaspar Matiasek Martin Alberer Laura Kolberg Ulrich von Both Katrin Hartmann Regina Hofmann-Lehmann |
| author_facet | Marina L. Meli Andrea M. Spiri Katharina Zwicklbauer Daniela Krentz Sandra Felten Michèle Bergmann Roswitha Dorsch Kaspar Matiasek Martin Alberer Laura Kolberg Ulrich von Both Katrin Hartmann Regina Hofmann-Lehmann |
| author_sort | Marina L. Meli |
| collection | DOAJ |
| description | As previously demonstrated by our research group, the oral multicomponent drug Xraphconn<sup>®</sup> containing GS-441524 was effective at curing otherwise fatal feline infectious peritonitis (FIP) in 18 feline coronavirus (FCoV)-infected cats. The aims of the current study were to investigate, using samples from the same animals as in the previous study, (1) the effect of treatment on fecal viral RNA shedding; (2) the presence of spike gene mutations in different body compartments of these cats; and (3) viral RNA shedding, presence of spike gene mutations, and anti-FCoV antibody titers in samples of 12 companion cats cohabitating with the treated cats. Eleven of the eighteen treated FIP cats (61%) were shedding FCoV RNA in feces within the first three days after treatment initiation, but all of them tested negative by day 6. In one of these cats, fecal shedding reoccurred on day 83. Two cats initially negative in feces were transiently positive 1–4 weeks into the study. The remaining five cats never shed FCoV. Viral RNA loads in feces decreased with time comparable with those in blood and effusion. Specific spike gene mutations linked to systemic FCoV spread were consistently found in blood and effusion from treated FIP cats, but not in feces from treated or companion cats. A new mutation that led to a not yet described amino acid change was identified, indicating that further mutations may be involved in the development of FIP. Eight of the twelve companion cats shed FCoV in feces. All but one of the twelve companion cats had anti-FCoV antibodies. Oral treatment with GS-441524 effectively decreased viral RNA loads in feces, blood, and effusion in cats with FIP. Nonetheless, re-shedding can most likely occur if cats are re-exposed to FCoV by their companion cats. |
| first_indexed | 2024-03-10T01:36:44Z |
| format | Article |
| id | doaj.art-b3719879ab3041c393a2d3d8b128de98 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:36:44Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b3719879ab3041c393a2d3d8b128de982023-11-23T13:32:51ZengMDPI AGViruses1999-49152022-05-01145106910.3390/v14051069Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524Marina L. Meli0Andrea M. Spiri1Katharina Zwicklbauer2Daniela Krentz3Sandra Felten4Michèle Bergmann5Roswitha Dorsch6Kaspar Matiasek7Martin Alberer8Laura Kolberg9Ulrich von Both10Katrin Hartmann11Regina Hofmann-Lehmann12Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, SwitzerlandClinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, SwitzerlandClinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, GermanyClinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, GermanyClinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, GermanyClinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, GermanyClinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, GermanySection of Clinical & Comparative Neuropathology, Institute of Veterinary Pathology, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, GermanyDivision of Paediatric Infectious Diseases, Dr. von Hauner Children’s Hospital, University Hospital, LMU-Munich, D-80337 Munich, GermanyDivision of Paediatric Infectious Diseases, Dr. von Hauner Children’s Hospital, University Hospital, LMU-Munich, D-80337 Munich, GermanyDivision of Paediatric Infectious Diseases, Dr. von Hauner Children’s Hospital, University Hospital, LMU-Munich, D-80337 Munich, GermanyClinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, GermanyClinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, SwitzerlandAs previously demonstrated by our research group, the oral multicomponent drug Xraphconn<sup>®</sup> containing GS-441524 was effective at curing otherwise fatal feline infectious peritonitis (FIP) in 18 feline coronavirus (FCoV)-infected cats. The aims of the current study were to investigate, using samples from the same animals as in the previous study, (1) the effect of treatment on fecal viral RNA shedding; (2) the presence of spike gene mutations in different body compartments of these cats; and (3) viral RNA shedding, presence of spike gene mutations, and anti-FCoV antibody titers in samples of 12 companion cats cohabitating with the treated cats. Eleven of the eighteen treated FIP cats (61%) were shedding FCoV RNA in feces within the first three days after treatment initiation, but all of them tested negative by day 6. In one of these cats, fecal shedding reoccurred on day 83. Two cats initially negative in feces were transiently positive 1–4 weeks into the study. The remaining five cats never shed FCoV. Viral RNA loads in feces decreased with time comparable with those in blood and effusion. Specific spike gene mutations linked to systemic FCoV spread were consistently found in blood and effusion from treated FIP cats, but not in feces from treated or companion cats. A new mutation that led to a not yet described amino acid change was identified, indicating that further mutations may be involved in the development of FIP. Eight of the twelve companion cats shed FCoV in feces. All but one of the twelve companion cats had anti-FCoV antibodies. Oral treatment with GS-441524 effectively decreased viral RNA loads in feces, blood, and effusion in cats with FIP. Nonetheless, re-shedding can most likely occur if cats are re-exposed to FCoV by their companion cats.https://www.mdpi.com/1999-4915/14/5/1069FIPFCoVsheddingviral loadsspike gene mutationstherapy |
| spellingShingle | Marina L. Meli Andrea M. Spiri Katharina Zwicklbauer Daniela Krentz Sandra Felten Michèle Bergmann Roswitha Dorsch Kaspar Matiasek Martin Alberer Laura Kolberg Ulrich von Both Katrin Hartmann Regina Hofmann-Lehmann Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524 Viruses FIP FCoV shedding viral loads spike gene mutations therapy |
| title | Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524 |
| title_full | Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524 |
| title_fullStr | Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524 |
| title_full_unstemmed | Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524 |
| title_short | Fecal Feline Coronavirus RNA Shedding and Spike Gene Mutations in Cats with Feline Infectious Peritonitis Treated with GS-441524 |
| title_sort | fecal feline coronavirus rna shedding and spike gene mutations in cats with feline infectious peritonitis treated with gs 441524 |
| topic | FIP FCoV shedding viral loads spike gene mutations therapy |
| url | https://www.mdpi.com/1999-4915/14/5/1069 |
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