Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity
Due to its increased prevalence, osteoporosis (OP) represents a great challenge to health care systems and brings an economic burden. To overcome these issues, treatment plans that suit the need of patients should be developed. One of the approaches focuses on the fabrication of personalized biomate...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/6/2095 |
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| author | Krzysztof Marycz Katarzyna Kornicka-Garbowska Adrian Patej Paulina Sobierajska Andrzej Kotela Eliza Turlej Martyna Kepska Alina Bienko Rafal J. Wiglusz |
| author_facet | Krzysztof Marycz Katarzyna Kornicka-Garbowska Adrian Patej Paulina Sobierajska Andrzej Kotela Eliza Turlej Martyna Kepska Alina Bienko Rafal J. Wiglusz |
| author_sort | Krzysztof Marycz |
| collection | DOAJ |
| description | Due to its increased prevalence, osteoporosis (OP) represents a great challenge to health care systems and brings an economic burden. To overcome these issues, treatment plans that suit the need of patients should be developed. One of the approaches focuses on the fabrication of personalized biomaterials, which can restore the balance and homeostasis of disease-affected bone. In the presented study, we fabricated nanometer crystalline hydroxyapatite (nHAp) and iron oxide (IO) nanoparticles stabilized with APTES and investigated whether they can modulate bone cell metabolism and be useful in the fabrication of personalized materials for OP patients. Using a wide range of molecular techniques, we have shown that obtained nHAp@APTES promotes viability and RUNX-2 expression in osteoblasts, as well as reducing activity of critical proinflammatory cytokines while inhibiting osteoclast activity. Materials with APTES modified with nHAp incorporated with IO nanoparticles can be applied to support the healing of osteoporotic bone fractures as they enhance metabolic activity of osteoblasts and diminish osteoclasts’ metabolism and inflammation. |
| first_indexed | 2024-03-09T13:29:31Z |
| format | Article |
| id | doaj.art-b373670195114ae6920bd1fa30d44671 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T13:29:31Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-b373670195114ae6920bd1fa30d446712023-11-30T21:19:32ZengMDPI AGMaterials1996-19442022-03-01156209510.3390/ma15062095Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast ActivityKrzysztof Marycz0Katarzyna Kornicka-Garbowska1Adrian Patej2Paulina Sobierajska3Andrzej Kotela4Eliza Turlej5Martyna Kepska6Alina Bienko7Rafal J. Wiglusz8The Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, PolandThe Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, PolandInstitute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, PolandInstitute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, PolandCollegium Medicum, Cardinal Stefan Wyszynski University (UKSW), Woycickiego 1/3, 01-938 Warsaw, PolandThe Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, PolandThe Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, PolandFaculty of Chemistry, University of Wroclaw, F. Joliot-Curie14 Street, 50-383 Wroclaw, PolandInstitute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, PolandDue to its increased prevalence, osteoporosis (OP) represents a great challenge to health care systems and brings an economic burden. To overcome these issues, treatment plans that suit the need of patients should be developed. One of the approaches focuses on the fabrication of personalized biomaterials, which can restore the balance and homeostasis of disease-affected bone. In the presented study, we fabricated nanometer crystalline hydroxyapatite (nHAp) and iron oxide (IO) nanoparticles stabilized with APTES and investigated whether they can modulate bone cell metabolism and be useful in the fabrication of personalized materials for OP patients. Using a wide range of molecular techniques, we have shown that obtained nHAp@APTES promotes viability and RUNX-2 expression in osteoblasts, as well as reducing activity of critical proinflammatory cytokines while inhibiting osteoclast activity. Materials with APTES modified with nHAp incorporated with IO nanoparticles can be applied to support the healing of osteoporotic bone fractures as they enhance metabolic activity of osteoblasts and diminish osteoclasts’ metabolism and inflammation.https://www.mdpi.com/1996-1944/15/6/2095osteoporosisosteoblastsosteoclastshydroxyapatiteAPTESiron oxides |
| spellingShingle | Krzysztof Marycz Katarzyna Kornicka-Garbowska Adrian Patej Paulina Sobierajska Andrzej Kotela Eliza Turlej Martyna Kepska Alina Bienko Rafal J. Wiglusz Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity Materials osteoporosis osteoblasts osteoclasts hydroxyapatite APTES iron oxides |
| title | Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity |
| title_full | Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity |
| title_fullStr | Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity |
| title_full_unstemmed | Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity |
| title_short | Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity |
| title_sort | aminopropyltriethoxysilane aptes modified nanohydroxyapatite nhap incorporated with iron oxide io nanoparticles promotes early osteogenesis reduces inflammation and inhibits osteoclast activity |
| topic | osteoporosis osteoblasts osteoclasts hydroxyapatite APTES iron oxides |
| url | https://www.mdpi.com/1996-1944/15/6/2095 |
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