Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers
ABSTRACT: To investigate the potential protective effect of intermittent cold stimulation on lung tissues of broilers exposed to acute cold stress (ACS). A total of 384 one-day-old broilers were assigned to 4 experimental groups with 6 replicates of 16 birds each: control (CON) and ACS groups were r...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123007654 |
| _version_ | 1827391258526482432 |
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| author | Haidong Wei Yong Zhang Tingting Li Shijie Zhang Jingwen Yin Yuanyuan Liu Lu Xing Jun Bao Jianhong Li |
| author_facet | Haidong Wei Yong Zhang Tingting Li Shijie Zhang Jingwen Yin Yuanyuan Liu Lu Xing Jun Bao Jianhong Li |
| author_sort | Haidong Wei |
| collection | DOAJ |
| description | ABSTRACT: To investigate the potential protective effect of intermittent cold stimulation on lung tissues of broilers exposed to acute cold stress (ACS). A total of 384 one-day-old broilers were assigned to 4 experimental groups with 6 replicates of 16 birds each: control (CON) and ACS groups were reared at normal feeding temperature from d 1 to 42; cold treatment groups (CS3+ACS and CS9+ACS) were reared, respectively, at 3°C or 9°C for 5 h on alternate days below the CON group from d 15 to 35. Animals in CS3+ACS, CS9+ACS, and ACS groups were exposed at 10°C for 24 h on d 43. Subsequently, lung tissues were collected to perform histopathological examination and measurement of relevant indexes. The results showed that lung tissues in CS9+ACS and ACS groups exhibited increased inflammatory cell infiltrates and collagen deposition compared to the CON group, while this pathological phenomenon was less pronounced in the CS3+ACS group. Compared to CON group, H2O2 and MDA contents were increased, and the activities of antioxidant enzymes (CAT, SOD, GPx, T-AOC) were reduced in CS9+ACS and ACS group (P < 0.05); mRNA and protein levels of inhibitor of NF-κB, Smad7, matrix metallopeptidase (MMP)-2, MMP9, and antioxidant-related genes were downregulated, whereas mRNA and protein levels of genes related to NF-κB/NLRP3 pathway-regulated inflammation and TGF-β1/Smad pathway-regulated fibrosis were upregulated in cold-stressed broilers (P < 0.05). mRNA levels of heme oxygenase-1, NAD(P)H quinone oxidoreductase-1, and MMP9 were increased in CS3+ACS group (P < 0.05). Moreover, the expression of most antioxidant-related genes was increased, and that of inflammation- and fibrosis-related genes was reduced in CS3+ACS group (P < 0.05). Therefore, cold stress caused oxidative stress and inflammation, leading to pulmonary fibrosis in broilers, whereas intermittent mild cold stimulation at 3°C below normal rearing temperature alleviated fibrosis by inhibiting the TGF-β1/Smad pathway modulated by the Nrf2/HO-1 and NF-κB/NLRP3 signaling pathway. This study suggests that intermittent mild cold stimulation can be a potential strategy to reduce ACS-induced lung damage in broilers. |
| first_indexed | 2024-03-08T17:07:56Z |
| format | Article |
| id | doaj.art-b378240eea554ddab6b5b9fd6aefab29 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T17:07:56Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-b378240eea554ddab6b5b9fd6aefab292024-01-04T04:35:44ZengElsevierPoultry Science0032-57912024-01-011031103246Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilersHaidong Wei0Yong Zhang1Tingting Li2Shijie Zhang3Jingwen Yin4Yuanyuan Liu5Lu Xing6Jun Bao7Jianhong Li8College of Life Science, Northeast Agricultural University, 150030 Harbin, ChinaCollege of Life Science, Northeast Agricultural University, 150030 Harbin, ChinaCollege of Life Science, Northeast Agricultural University, 150030 Harbin, ChinaCollege of Life Science, Northeast Agricultural University, 150030 Harbin, ChinaCollege of Life Science, Northeast Agricultural University, 150030 Harbin, ChinaCollege of Life Science, Northeast Agricultural University, 150030 Harbin, ChinaCollege of Life Science, Northeast Agricultural University, 150030 Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, 150030 Harbin, China; Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, 150030 Harbin, ChinaCollege of Life Science, Northeast Agricultural University, 150030 Harbin, China; Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, 150030 Harbin, China; Corresponding author:ABSTRACT: To investigate the potential protective effect of intermittent cold stimulation on lung tissues of broilers exposed to acute cold stress (ACS). A total of 384 one-day-old broilers were assigned to 4 experimental groups with 6 replicates of 16 birds each: control (CON) and ACS groups were reared at normal feeding temperature from d 1 to 42; cold treatment groups (CS3+ACS and CS9+ACS) were reared, respectively, at 3°C or 9°C for 5 h on alternate days below the CON group from d 15 to 35. Animals in CS3+ACS, CS9+ACS, and ACS groups were exposed at 10°C for 24 h on d 43. Subsequently, lung tissues were collected to perform histopathological examination and measurement of relevant indexes. The results showed that lung tissues in CS9+ACS and ACS groups exhibited increased inflammatory cell infiltrates and collagen deposition compared to the CON group, while this pathological phenomenon was less pronounced in the CS3+ACS group. Compared to CON group, H2O2 and MDA contents were increased, and the activities of antioxidant enzymes (CAT, SOD, GPx, T-AOC) were reduced in CS9+ACS and ACS group (P < 0.05); mRNA and protein levels of inhibitor of NF-κB, Smad7, matrix metallopeptidase (MMP)-2, MMP9, and antioxidant-related genes were downregulated, whereas mRNA and protein levels of genes related to NF-κB/NLRP3 pathway-regulated inflammation and TGF-β1/Smad pathway-regulated fibrosis were upregulated in cold-stressed broilers (P < 0.05). mRNA levels of heme oxygenase-1, NAD(P)H quinone oxidoreductase-1, and MMP9 were increased in CS3+ACS group (P < 0.05). Moreover, the expression of most antioxidant-related genes was increased, and that of inflammation- and fibrosis-related genes was reduced in CS3+ACS group (P < 0.05). Therefore, cold stress caused oxidative stress and inflammation, leading to pulmonary fibrosis in broilers, whereas intermittent mild cold stimulation at 3°C below normal rearing temperature alleviated fibrosis by inhibiting the TGF-β1/Smad pathway modulated by the Nrf2/HO-1 and NF-κB/NLRP3 signaling pathway. This study suggests that intermittent mild cold stimulation can be a potential strategy to reduce ACS-induced lung damage in broilers.http://www.sciencedirect.com/science/article/pii/S0032579123007654broiler lungcold stimulationfibrosisinflammationoxidative stress |
| spellingShingle | Haidong Wei Yong Zhang Tingting Li Shijie Zhang Jingwen Yin Yuanyuan Liu Lu Xing Jun Bao Jianhong Li Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers Poultry Science broiler lung cold stimulation fibrosis inflammation oxidative stress |
| title | Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers |
| title_full | Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers |
| title_fullStr | Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers |
| title_full_unstemmed | Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers |
| title_short | Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers |
| title_sort | intermittent mild cold stimulation alleviates cold stress induced pulmonary fibrosis by inhibiting the tgf β1 smad signaling pathway in broilers |
| topic | broiler lung cold stimulation fibrosis inflammation oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123007654 |
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