Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1
In this study, a sensitive and specific monoclonal antibody against VIR M1 was produced based on a new hapten. The antibody had a linear detection range of 3.77–65.51 ng/mL with an IC50 of 15.26 ng/mL and good specificity for VIR M1. Gold nanoparticle (GNP)-based lateral flow strips were assembled f...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1763262 |
| _version_ | 1818640731669004288 |
|---|---|
| author | Xiaoxin Xu Xiaoling Wu Hua Kuang Shanshan Song |
| author_facet | Xiaoxin Xu Xiaoling Wu Hua Kuang Shanshan Song |
| author_sort | Xiaoxin Xu |
| collection | DOAJ |
| description | In this study, a sensitive and specific monoclonal antibody against VIR M1 was produced based on a new hapten. The antibody had a linear detection range of 3.77–65.51 ng/mL with an IC50 of 15.26 ng/mL and good specificity for VIR M1. Gold nanoparticle (GNP)-based lateral flow strips were assembled for rapid screening of VIR M1 in feed samples. When evaluated with the naked eye, the visual limit of detection (LOD) in feed samples was 25 μg/kg, and the cut-off value was 200 μg/kg. Therefore, this GNP-based lateral flow strip assay can be used for on-site detection and rapid preliminary screening of VIR M1 in feed samples. |
| first_indexed | 2024-12-16T23:15:56Z |
| format | Article |
| id | doaj.art-b3787d3bb71b4a9fa407cdbe8075e512 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-16T23:15:56Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-b3787d3bb71b4a9fa407cdbe8075e5122022-12-21T22:12:17ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131176477710.1080/09540105.2020.17632621763262Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1Xiaoxin Xu0Xiaoling Wu1Hua Kuang2Shanshan Song3State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityIn this study, a sensitive and specific monoclonal antibody against VIR M1 was produced based on a new hapten. The antibody had a linear detection range of 3.77–65.51 ng/mL with an IC50 of 15.26 ng/mL and good specificity for VIR M1. Gold nanoparticle (GNP)-based lateral flow strips were assembled for rapid screening of VIR M1 in feed samples. When evaluated with the naked eye, the visual limit of detection (LOD) in feed samples was 25 μg/kg, and the cut-off value was 200 μg/kg. Therefore, this GNP-based lateral flow strip assay can be used for on-site detection and rapid preliminary screening of VIR M1 in feed samples.http://dx.doi.org/10.1080/09540105.2020.1763262virginiamycin m1monoclonal antibodiesgold nanoparticle-based lateral flow stripsamples |
| spellingShingle | Xiaoxin Xu Xiaoling Wu Hua Kuang Shanshan Song Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1 Food and Agricultural Immunology virginiamycin m1 monoclonal antibodies gold nanoparticle-based lateral flow strip samples |
| title | Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1 |
| title_full | Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1 |
| title_fullStr | Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1 |
| title_full_unstemmed | Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1 |
| title_short | Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1 |
| title_sort | gold nanoparticle based lateral flow strips for rapid and sensitive detection of virginiamycin m1 |
| topic | virginiamycin m1 monoclonal antibodies gold nanoparticle-based lateral flow strip samples |
| url | http://dx.doi.org/10.1080/09540105.2020.1763262 |
| work_keys_str_mv | AT xiaoxinxu goldnanoparticlebasedlateralflowstripsforrapidandsensitivedetectionofvirginiamycinm1 AT xiaolingwu goldnanoparticlebasedlateralflowstripsforrapidandsensitivedetectionofvirginiamycinm1 AT huakuang goldnanoparticlebasedlateralflowstripsforrapidandsensitivedetectionofvirginiamycinm1 AT shanshansong goldnanoparticlebasedlateralflowstripsforrapidandsensitivedetectionofvirginiamycinm1 |