Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq
The presented investigation was performed to identify Sarcocystis species, in an intermediate-host parasite with zoonotic importance, infection in slaughtered sheep identified by traditional, histological, and molecular methods in Al-Diwaniyah Province, Iraq. Here, 70 intercostal and esophageal musc...
| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-10-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_180563_a119b4587c37a9db1378484066816d22.pdf |
| _version_ | 1797669193559048192 |
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| author | Hadeel H. Albayati Ghaidaa A. Jassem |
| author_facet | Hadeel H. Albayati Ghaidaa A. Jassem |
| author_sort | Hadeel H. Albayati |
| collection | DOAJ |
| description | The presented investigation was performed to identify Sarcocystis species, in an intermediate-host parasite with zoonotic importance, infection in slaughtered sheep identified by traditional, histological, and molecular methods in Al-Diwaniyah Province, Iraq. Here, 70 intercostal and esophageal muscle samples 10-40 gm/each were collected from slaughtered sheep. The samples were examined for detection of the Sarcocystic bradyzoites using microscopic, histological, 18S rRNA gene-based polymerase chain reaction (PCR) and PCR-product-dependent gene sequencing methods. The results of the microscopic method revealed that 65 (93%) of the muscle samples contained bradyzoites. The histopathological picture showed the presence of two microsarcocysts with different morphological thin or thick wall sizes. The PCR demonstrated that 68/70 (97%) of the samples were positive for the occurrence of the parasite DNA. According to the NCBI-based websites, the Phylogenetic analysis revealed that the isolates from the current study were closely similar in their nucleotide sequences with isolates from Norway, Egypt, and Iran. The present data indicate high infection levels by Sarcocystis spp. in slaughtered sheep, which could bring alarm for public health importance. |
| first_indexed | 2024-03-11T20:40:36Z |
| format | Article |
| id | doaj.art-b37f50ec0ae046ff9247742f5152211e |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-03-11T20:40:36Z |
| publishDate | 2023-10-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-b37f50ec0ae046ff9247742f5152211e2023-10-02T03:07:56ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-10-0137487187510.33899/ijvs.2023.138763.2835180563Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, IraqHadeel H. Albayati0Ghaidaa A. Jassem1Department of Veterinary Microbiology, College of Veterinary Medicine, University of Al-Qadisiya, Al-Diwaniyah, IraqDepartment of Veterinary Microbiology, College of Veterinary Medicine, University of Al-Qadisiya, Al-Diwaniyah, IraqThe presented investigation was performed to identify Sarcocystis species, in an intermediate-host parasite with zoonotic importance, infection in slaughtered sheep identified by traditional, histological, and molecular methods in Al-Diwaniyah Province, Iraq. Here, 70 intercostal and esophageal muscle samples 10-40 gm/each were collected from slaughtered sheep. The samples were examined for detection of the Sarcocystic bradyzoites using microscopic, histological, 18S rRNA gene-based polymerase chain reaction (PCR) and PCR-product-dependent gene sequencing methods. The results of the microscopic method revealed that 65 (93%) of the muscle samples contained bradyzoites. The histopathological picture showed the presence of two microsarcocysts with different morphological thin or thick wall sizes. The PCR demonstrated that 68/70 (97%) of the samples were positive for the occurrence of the parasite DNA. According to the NCBI-based websites, the Phylogenetic analysis revealed that the isolates from the current study were closely similar in their nucleotide sequences with isolates from Norway, Egypt, and Iran. The present data indicate high infection levels by Sarcocystis spp. in slaughtered sheep, which could bring alarm for public health importance.https://www.vetmedmosul.com/article_180563_a119b4587c37a9db1378484066816d22.pdfbradyzoitesarcocystis sppzoonotic diseasesqueezing test |
| spellingShingle | Hadeel H. Albayati Ghaidaa A. Jassem Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq Iraqi Journal of Veterinary Sciences bradyzoite sarcocystis spp zoonotic disease squeezing test |
| title | Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq |
| title_full | Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq |
| title_fullStr | Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq |
| title_full_unstemmed | Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq |
| title_short | Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq |
| title_sort | traditional histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in al diwaniyah province iraq |
| topic | bradyzoite sarcocystis spp zoonotic disease squeezing test |
| url | https://www.vetmedmosul.com/article_180563_a119b4587c37a9db1378484066816d22.pdf |
| work_keys_str_mv | AT hadeelhalbayati traditionalhistopathologicalandmoleculardiagnosisofsarcocytosisinslaughteredsheepinaldiwaniyahprovinceiraq AT ghaidaaajassem traditionalhistopathologicalandmoleculardiagnosisofsarcocytosisinslaughteredsheepinaldiwaniyahprovinceiraq |