Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases
Ocular sarcoidosis is an inflammatory disease that manifests as uveitis, and is often difficult to distinguish from other forms of uveitis based on nonspecific findings alone. Comprehensive proteomic analyses of vitreous humor using LC-MS/MS were performed in each patient with ocular sarcoidosis, vi...
| Main Authors: | , , , , , , , , , , , , |
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MDPI AG
2022-06-01
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| Series: | Journal of Clinical Medicine |
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| author | Hiroyuki Komatsu Yoshihiko Usui Kinya Tsubota Risa Fujii Takefumi Yamaguchi Kazuichi Maruyama Ryo Wakita Masaki Asakage Hiroyuki Shimizu Naoyuki Yamakawa Naoya Nezu Koji Ueda Hiroshi Goto |
| author_facet | Hiroyuki Komatsu Yoshihiko Usui Kinya Tsubota Risa Fujii Takefumi Yamaguchi Kazuichi Maruyama Ryo Wakita Masaki Asakage Hiroyuki Shimizu Naoyuki Yamakawa Naoya Nezu Koji Ueda Hiroshi Goto |
| author_sort | Hiroyuki Komatsu |
| collection | DOAJ |
| description | Ocular sarcoidosis is an inflammatory disease that manifests as uveitis, and is often difficult to distinguish from other forms of uveitis based on nonspecific findings alone. Comprehensive proteomic analyses of vitreous humor using LC-MS/MS were performed in each patient with ocular sarcoidosis, vitreoretinal lymphoma (VRL), and controls with epiretinal membrane or macular hole. Differential expression proteins (DEPs) were identified by comparing with VRL and controls, and functional pathway analysis was performed. The candidate biomarker proteins for ocular sarcoidosis were validated using enzyme-linked immunosorbent assay. A total of 1590 proteins were identified in all samples. Of these, 290 and 174 DEPs were detected in vitreous of ocular sarcoidosis compared with controls and VRL, respectively. Enrichment pathway analysis revealed that pathways related to the immune system were most upregulated. Validation of two candidate biomarkers for ocular sarcoidosis, neutrophil gelatinase-associated lipocalin (NGAL) and junctional adhesion molecules B (JAMB), confirmed upregulated NGAL and JAMB protein expressions in ocular sarcoidosis compared to controls and VRL. The results of this study revealed that altered vitreous protein expression levels may discriminate ocular sarcoidosis from other uveitis diseases. Vitreous NGAL and JAMB are potential biomarkers and may serve as an auxiliary tool for the diagnosis of ocular sarcoidosis. |
| first_indexed | 2024-03-09T12:51:14Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T12:51:14Z |
| publishDate | 2022-06-01 |
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| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-b37f7242027b4c63957c809ea0de169a2023-11-30T22:06:15ZengMDPI AGJournal of Clinical Medicine2077-03832022-06-011113360610.3390/jcm11133606Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal DiseasesHiroyuki Komatsu0Yoshihiko Usui1Kinya Tsubota2Risa Fujii3Takefumi Yamaguchi4Kazuichi Maruyama5Ryo Wakita6Masaki Asakage7Hiroyuki Shimizu8Naoyuki Yamakawa9Naoya Nezu10Koji Ueda11Hiroshi Goto12Department of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanProject for Realization of Personalized Cancer Medicine, Cancer Precision Medical Center, Japanese Foundation for Cancer Research, Tokyo 135-8550, JapanDepartment of Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Chiba 272-8513, JapanDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanProject for Realization of Personalized Cancer Medicine, Cancer Precision Medical Center, Japanese Foundation for Cancer Research, Tokyo 135-8550, JapanDepartment of Ophthalmology, Tokyo Medical University, Tokyo 160-8402, JapanOcular sarcoidosis is an inflammatory disease that manifests as uveitis, and is often difficult to distinguish from other forms of uveitis based on nonspecific findings alone. Comprehensive proteomic analyses of vitreous humor using LC-MS/MS were performed in each patient with ocular sarcoidosis, vitreoretinal lymphoma (VRL), and controls with epiretinal membrane or macular hole. Differential expression proteins (DEPs) were identified by comparing with VRL and controls, and functional pathway analysis was performed. The candidate biomarker proteins for ocular sarcoidosis were validated using enzyme-linked immunosorbent assay. A total of 1590 proteins were identified in all samples. Of these, 290 and 174 DEPs were detected in vitreous of ocular sarcoidosis compared with controls and VRL, respectively. Enrichment pathway analysis revealed that pathways related to the immune system were most upregulated. Validation of two candidate biomarkers for ocular sarcoidosis, neutrophil gelatinase-associated lipocalin (NGAL) and junctional adhesion molecules B (JAMB), confirmed upregulated NGAL and JAMB protein expressions in ocular sarcoidosis compared to controls and VRL. The results of this study revealed that altered vitreous protein expression levels may discriminate ocular sarcoidosis from other uveitis diseases. Vitreous NGAL and JAMB are potential biomarkers and may serve as an auxiliary tool for the diagnosis of ocular sarcoidosis.https://www.mdpi.com/2077-0383/11/13/3606proteomics analysisocular sarcoidosisuveitisvitreoretinal diseasesvitreoretinal lymphoma |
| spellingShingle | Hiroyuki Komatsu Yoshihiko Usui Kinya Tsubota Risa Fujii Takefumi Yamaguchi Kazuichi Maruyama Ryo Wakita Masaki Asakage Hiroyuki Shimizu Naoyuki Yamakawa Naoya Nezu Koji Ueda Hiroshi Goto Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases Journal of Clinical Medicine proteomics analysis ocular sarcoidosis uveitis vitreoretinal diseases vitreoretinal lymphoma |
| title | Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases |
| title_full | Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases |
| title_fullStr | Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases |
| title_full_unstemmed | Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases |
| title_short | Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases |
| title_sort | comprehensive proteomic profiling of vitreous humor in ocular sarcoidosis compared with other vitreoretinal diseases |
| topic | proteomics analysis ocular sarcoidosis uveitis vitreoretinal diseases vitreoretinal lymphoma |
| url | https://www.mdpi.com/2077-0383/11/13/3606 |
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