Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small Ruminants
Haemonchosis is a parasitic disease of small ruminants that adversely affects livestock production. <i>Haemonchus contortus</i> is one of the most prevalent nematode parasites that infect the abomasum of small ruminants. This parasite reduces milk production, overall growth and sometimes...
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1843 |
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| author | Asfa Nazish Fozia Baharullah Khattak Taj Ali Khan Ijaz Ahmad Riaz Ullah Ahmed Bari Majid M. Asmari Hafiz M. Mahmood Muhammad Sohaib Ahmad El Askary Attalla F. El-kott Mohamed M. Abdel-Daim |
| author_facet | Asfa Nazish Fozia Baharullah Khattak Taj Ali Khan Ijaz Ahmad Riaz Ullah Ahmed Bari Majid M. Asmari Hafiz M. Mahmood Muhammad Sohaib Ahmad El Askary Attalla F. El-kott Mohamed M. Abdel-Daim |
| author_sort | Asfa Nazish |
| collection | DOAJ |
| description | Haemonchosis is a parasitic disease of small ruminants that adversely affects livestock production. <i>Haemonchus contortus</i> is one of the most prevalent nematode parasites that infect the abomasum of small ruminants. This parasite reduces milk production, overall growth and sometimes causes the death of the infected animals. The evaluation of the biocontrol potential of some abomasum bacterial isolates against <i>H. contortus</i> is investigated in this study. Out of which, three isolates—<i>Comamonas testosteroni</i>, <i>Comamonas jiangduensis</i>, <i>Pseudomonas weihenstephanesis</i>—show significant effect against the nematode L3, adult, and egg hatch inhibition assays. Various concentrations of metabolites from these bacteria are prepared and applied in different treatments compared with control. In the case of adult mortality assay, 50% metabolites of <i>C. testosteroni</i> and <i>P. weihenstephanesis</i> show 46% adult mortality, whereas <i>C. jiangduensis</i> shows 40% mortality. It is observed that decreasing the concentration of bacterial metabolite, lowers nematode mortality. The minimum nematode mortality rate is recorded at the lowest filtrates concentration of all the bacterial isolates. The same trend is observed in egg hatch inhibition assay, where the higher concentration of bacterial culture filtrates shows 100% inhibition of <i>H. contortus</i> egg. It is concluded that the effect of bacterial culture filtrates against <i>H. contortus</i> is dose-dependent for their activity against nematode L3, adult, and inhibition of egg hatchment. |
| first_indexed | 2024-03-10T10:12:55Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:12:55Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-b380c721699b416d9a93b794e4354d702023-11-22T01:03:58ZengMDPI AGAnimals2076-26152021-06-01116184310.3390/ani11061843Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small RuminantsAsfa Nazish0Fozia1Baharullah Khattak2Taj Ali Khan3Ijaz Ahmad4Riaz Ullah5Ahmed Bari6Majid M. Asmari7Hafiz M. Mahmood8Muhammad Sohaib9Ahmad El Askary10Attalla F. El-kott11Mohamed M. Abdel-Daim12Department of Microbiology, Kohat University of Science & Technology, Kohat 26000, PakistanBoichemistry Department, KMU Institute of Medical Sciences, Kohat 26000, PakistanDepartment of Microbiology, Kohat University of Science & Technology, Kohat 26000, PakistanDepartment of Microbiology, Kohat University of Science & Technology, Kohat 26000, PakistanDepartment of Chemistry, Kohat University of Science & Technology, Kohat 26000, PakistanDepartment of Chemistry, Kohat University of Science & Technology, Kohat 26000, PakistanDepartment of Chemistry, Kohat University of Science & Technology, Kohat 26000, PakistanDepartment of Pharmaceutical Chemistry, College of Pharmacy, P.O. Box 2457, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Soil Science, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, Abha 61421, Saudi ArabiaPharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptHaemonchosis is a parasitic disease of small ruminants that adversely affects livestock production. <i>Haemonchus contortus</i> is one of the most prevalent nematode parasites that infect the abomasum of small ruminants. This parasite reduces milk production, overall growth and sometimes causes the death of the infected animals. The evaluation of the biocontrol potential of some abomasum bacterial isolates against <i>H. contortus</i> is investigated in this study. Out of which, three isolates—<i>Comamonas testosteroni</i>, <i>Comamonas jiangduensis</i>, <i>Pseudomonas weihenstephanesis</i>—show significant effect against the nematode L3, adult, and egg hatch inhibition assays. Various concentrations of metabolites from these bacteria are prepared and applied in different treatments compared with control. In the case of adult mortality assay, 50% metabolites of <i>C. testosteroni</i> and <i>P. weihenstephanesis</i> show 46% adult mortality, whereas <i>C. jiangduensis</i> shows 40% mortality. It is observed that decreasing the concentration of bacterial metabolite, lowers nematode mortality. The minimum nematode mortality rate is recorded at the lowest filtrates concentration of all the bacterial isolates. The same trend is observed in egg hatch inhibition assay, where the higher concentration of bacterial culture filtrates shows 100% inhibition of <i>H. contortus</i> egg. It is concluded that the effect of bacterial culture filtrates against <i>H. contortus</i> is dose-dependent for their activity against nematode L3, adult, and inhibition of egg hatchment.https://www.mdpi.com/2076-2615/11/6/1843small ruminants<i>H. contortus</i>abomasumfecal samplesbacterial culture filtrates |
| spellingShingle | Asfa Nazish Fozia Baharullah Khattak Taj Ali Khan Ijaz Ahmad Riaz Ullah Ahmed Bari Majid M. Asmari Hafiz M. Mahmood Muhammad Sohaib Ahmad El Askary Attalla F. El-kott Mohamed M. Abdel-Daim Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small Ruminants Animals small ruminants <i>H. contortus</i> abomasum fecal samples bacterial culture filtrates |
| title | Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small Ruminants |
| title_full | Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small Ruminants |
| title_fullStr | Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small Ruminants |
| title_full_unstemmed | Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small Ruminants |
| title_short | Antinematode Activity of Abomasum Bacterial Culture Filtrates against <i>Haemonchus contortus</i> in Small Ruminants |
| title_sort | antinematode activity of abomasum bacterial culture filtrates against i haemonchus contortus i in small ruminants |
| topic | small ruminants <i>H. contortus</i> abomasum fecal samples bacterial culture filtrates |
| url | https://www.mdpi.com/2076-2615/11/6/1843 |
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