Detection of DNA methylation signatures through the lens of genomic imprinting
Abstract Genomic imprinting represents an original model of epigenetic regulation resulting in a parent-of-origin expression. Despite the critical role of imprinted genes in mammalian growth, metabolism and neuronal function, there is no molecular tool specifically targeting them for a systematic ev...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52114-3 |
| _version_ | 1797350017174863872 |
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| author | Jean-Noël Hubert Nathalie Iannuccelli Cédric Cabau Eva Jacomet Yvon Billon Rémy-Félix Serre Céline Vandecasteele Cécile Donnadieu Julie Demars |
| author_facet | Jean-Noël Hubert Nathalie Iannuccelli Cédric Cabau Eva Jacomet Yvon Billon Rémy-Félix Serre Céline Vandecasteele Cécile Donnadieu Julie Demars |
| author_sort | Jean-Noël Hubert |
| collection | DOAJ |
| description | Abstract Genomic imprinting represents an original model of epigenetic regulation resulting in a parent-of-origin expression. Despite the critical role of imprinted genes in mammalian growth, metabolism and neuronal function, there is no molecular tool specifically targeting them for a systematic evaluation. We show here that enzymatic methyl-seq consistently outperforms the bisulfite-based standard in capturing 165 candidate regions for genomic imprinting in the pig. This highlights the potential for a turnkey, fully customizable and reliable capture tool of genomic regions regulated by cytosine methylation in any population of interest. For the field of genomic imprinting, it opens up the possibility of detecting multilocus imprinting variations across the genome, with implications for basic research, agrigenomics and clinical practice. |
| first_indexed | 2024-03-08T12:38:42Z |
| format | Article |
| id | doaj.art-b3849b135ebe431cb05a3c61aa19df99 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T12:38:42Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b3849b135ebe431cb05a3c61aa19df992024-01-21T12:18:10ZengNature PortfolioScientific Reports2045-23222024-01-011411810.1038/s41598-024-52114-3Detection of DNA methylation signatures through the lens of genomic imprintingJean-Noël Hubert0Nathalie Iannuccelli1Cédric Cabau2Eva Jacomet3Yvon Billon4Rémy-Félix Serre5Céline Vandecasteele6Cécile Donnadieu7Julie Demars8GenPhySE, Université de Toulouse, INRAE, ENVTGenPhySE, Université de Toulouse, INRAE, ENVTSigenae, GenPhySE, Université de Toulouse, INRAE, ENVTGenPhySE, Université de Toulouse, INRAE, ENVTINRAE, GenESIINRAE, GeT-PlaGe, GenotoulINRAE, GeT-PlaGe, GenotoulINRAE, GeT-PlaGe, GenotoulGenPhySE, Université de Toulouse, INRAE, ENVTAbstract Genomic imprinting represents an original model of epigenetic regulation resulting in a parent-of-origin expression. Despite the critical role of imprinted genes in mammalian growth, metabolism and neuronal function, there is no molecular tool specifically targeting them for a systematic evaluation. We show here that enzymatic methyl-seq consistently outperforms the bisulfite-based standard in capturing 165 candidate regions for genomic imprinting in the pig. This highlights the potential for a turnkey, fully customizable and reliable capture tool of genomic regions regulated by cytosine methylation in any population of interest. For the field of genomic imprinting, it opens up the possibility of detecting multilocus imprinting variations across the genome, with implications for basic research, agrigenomics and clinical practice.https://doi.org/10.1038/s41598-024-52114-3 |
| spellingShingle | Jean-Noël Hubert Nathalie Iannuccelli Cédric Cabau Eva Jacomet Yvon Billon Rémy-Félix Serre Céline Vandecasteele Cécile Donnadieu Julie Demars Detection of DNA methylation signatures through the lens of genomic imprinting Scientific Reports |
| title | Detection of DNA methylation signatures through the lens of genomic imprinting |
| title_full | Detection of DNA methylation signatures through the lens of genomic imprinting |
| title_fullStr | Detection of DNA methylation signatures through the lens of genomic imprinting |
| title_full_unstemmed | Detection of DNA methylation signatures through the lens of genomic imprinting |
| title_short | Detection of DNA methylation signatures through the lens of genomic imprinting |
| title_sort | detection of dna methylation signatures through the lens of genomic imprinting |
| url | https://doi.org/10.1038/s41598-024-52114-3 |
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