Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS
The ultrasonic cell disruption method was used to efficiently extract isothiocyanates and other volatile compounds from radish microgreens. A total of 51 volatiles were identified and quantified by headspace solid-phase micro-extraction and gas chromatography–mass spectrometry (HS-SPME/GC–MS) in fou...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/19/14988 |
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| author | Yuan Zhong Zhilong Jia Hailong Zhou Dan Zhang Guichen Li Jihua Yu |
| author_facet | Yuan Zhong Zhilong Jia Hailong Zhou Dan Zhang Guichen Li Jihua Yu |
| author_sort | Yuan Zhong |
| collection | DOAJ |
| description | The ultrasonic cell disruption method was used to efficiently extract isothiocyanates and other volatile compounds from radish microgreens. A total of 51 volatiles were identified and quantified by headspace solid-phase micro-extraction and gas chromatography–mass spectrometry (HS-SPME/GC–MS) in four radish microgreen cultivars, mainly including alcohols, aldehydes, isothiocyanates, sulfides, ketones, esters, terpenes, and hydrocarbons. The correlation between cultivars and volatile compounds was determined by chemometrics analysis, including principal component analysis (PCA) and hierarchical clustering heat maps. The aroma profiles were distinguished based on the odor activity value (OAV), odor contribution rate (OCR), and radar fingerprint chart (RFC) of volatile compounds. This study not only revealed the different flavor characteristics in four cultivars but also established a theoretical basis for the genetic improvement of radish microgreen flavors. |
| first_indexed | 2024-03-10T21:43:01Z |
| format | Article |
| id | doaj.art-b3868cab0405451396b6cf7d1a903627 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:43:01Z |
| publishDate | 2023-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b3868cab0405451396b6cf7d1a9036272023-11-19T14:33:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124191498810.3390/ijms241914988Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MSYuan Zhong0Zhilong Jia1Hailong Zhou2Dan Zhang3Guichen Li4Jihua Yu5State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaThe ultrasonic cell disruption method was used to efficiently extract isothiocyanates and other volatile compounds from radish microgreens. A total of 51 volatiles were identified and quantified by headspace solid-phase micro-extraction and gas chromatography–mass spectrometry (HS-SPME/GC–MS) in four radish microgreen cultivars, mainly including alcohols, aldehydes, isothiocyanates, sulfides, ketones, esters, terpenes, and hydrocarbons. The correlation between cultivars and volatile compounds was determined by chemometrics analysis, including principal component analysis (PCA) and hierarchical clustering heat maps. The aroma profiles were distinguished based on the odor activity value (OAV), odor contribution rate (OCR), and radar fingerprint chart (RFC) of volatile compounds. This study not only revealed the different flavor characteristics in four cultivars but also established a theoretical basis for the genetic improvement of radish microgreen flavors.https://www.mdpi.com/1422-0067/24/19/14988flavorvolatile compoundsradish microgreensultrasonic cell disruptionGC–MS |
| spellingShingle | Yuan Zhong Zhilong Jia Hailong Zhou Dan Zhang Guichen Li Jihua Yu Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS International Journal of Molecular Sciences flavor volatile compounds radish microgreens ultrasonic cell disruption GC–MS |
| title | Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS |
| title_full | Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS |
| title_fullStr | Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS |
| title_full_unstemmed | Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS |
| title_short | Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS |
| title_sort | comparative analysis of volatile compounds from four radish microgreen cultivars based on ultrasonic cell disruption and hs spme gc ms |
| topic | flavor volatile compounds radish microgreens ultrasonic cell disruption GC–MS |
| url | https://www.mdpi.com/1422-0067/24/19/14988 |
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