Efficient generation of targeted point mutations in the Brassica oleracea var. botrytis genome via a modified CRISPR/Cas9 system

Efficient generation of targeted point mutations in the Brassica oleracea var. botrytis genome via a modified CRISPR/Cas9 system

In this study, we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower. Acetolactate synthase (ALS) and Centromere-specific histone H3 variant (CENH3) genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants. For ALS gene,...

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Bibliographic Details
Main Authors: Guixiang Wang, Mei Zong, Di Liu, Yage Wu, Shouwei Tian, Shuo Han, Ning Guo, Mengmeng Duan, Liming Miao, Fan Liu
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2022-07-01
Series:Horticultural Plant Journal
Subjects:
Cauliflower
Targeted point mutations
Base-editing
CRISPR/Cas9
ALS
CENH3
Online Access:http://www.sciencedirect.com/science/article/pii/S2468014122000139

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