In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-04-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/4/232 |
| _version_ | 1828295703024107520 |
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| author | Sylvia G. Lehmann Benjamin Gilbert Thierry GG Maffeis Alexei Grichine Isabelle Pignot-Paintrand Simon Clavaguera Walid Rachidi Michel Seve Laurent Charlet |
| author_facet | Sylvia G. Lehmann Benjamin Gilbert Thierry GG Maffeis Alexei Grichine Isabelle Pignot-Paintrand Simon Clavaguera Walid Rachidi Michel Seve Laurent Charlet |
| author_sort | Sylvia G. Lehmann |
| collection | DOAJ |
| description | Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies that have specifically evaluated the potential toxicity from dermal exposure. To address this question, we investigated the dermal toxicity after acute exposure of polymer-coated AgNW with two sizes using two models, human primary keratinocytes and human reconstructed epidermis. In keratinocytes, AgNW are rapidly and massively internalized inside cells leading to dose-dependent cytotoxicity that was not due to Ag+ release. Analysing our data with different dose metrics, we propose that the number of NW is the most appropriate dose-metric for studies of AgNW toxicity. In reconstructed epidermis, the results of a standard in vitro skin irritation assay classified AgNW as non-irritant to skin and we found no evidence of penetration into the deeper layer of the epidermis. The findings show that healthy and intact epidermis provides an effective barrier for AgNW, although the study does not address potential transport through follicles or injured skin. The combined cell and tissue model approach used here is likely to provide an important methodology for assessing the risks for skin exposure to AgNW from consumer products. |
| first_indexed | 2024-04-13T11:59:32Z |
| format | Article |
| id | doaj.art-b38decb4a2d6409db496a7c94239ebc7 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-04-13T11:59:32Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b38decb4a2d6409db496a7c94239ebc72022-12-22T02:47:49ZengMDPI AGNanomaterials2079-49912018-04-018423210.3390/nano8040232nano8040232In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell ModelsSylvia G. Lehmann0Benjamin Gilbert1Thierry GG Maffeis2Alexei Grichine3Isabelle Pignot-Paintrand4Simon Clavaguera5Walid Rachidi6Michel Seve7Laurent Charlet8Univ. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, IRD, IFSTTAR, ISTerre, 38000 Grenoble, FranceUniv. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, IRD, IFSTTAR, ISTerre, 38000 Grenoble, FranceCentre for Nanohealth, College of Engineering, Swansea University, Swansea SA1 8EN, UKINSERM, UGA, Institute for Advanced Biosciences, F-38000 Grenoble, FranceUniv. Grenoble Alpes, CNRS, Grenoble INP, F-38000 Grenoble, FranceUniv. Grenoble Alpes, F-38000 Grenoble, FranceUniv. Grenoble Alpes, INAC, SyMMES, F-38000 Grenoble, FranceUniv. Grenoble Alpes, LBFA et BEeSy, PROMETHEE Proteomic Platform, F-38000 Grenoble, FranceUniv. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, IRD, IFSTTAR, ISTerre, 38000 Grenoble, FranceSilver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies that have specifically evaluated the potential toxicity from dermal exposure. To address this question, we investigated the dermal toxicity after acute exposure of polymer-coated AgNW with two sizes using two models, human primary keratinocytes and human reconstructed epidermis. In keratinocytes, AgNW are rapidly and massively internalized inside cells leading to dose-dependent cytotoxicity that was not due to Ag+ release. Analysing our data with different dose metrics, we propose that the number of NW is the most appropriate dose-metric for studies of AgNW toxicity. In reconstructed epidermis, the results of a standard in vitro skin irritation assay classified AgNW as non-irritant to skin and we found no evidence of penetration into the deeper layer of the epidermis. The findings show that healthy and intact epidermis provides an effective barrier for AgNW, although the study does not address potential transport through follicles or injured skin. The combined cell and tissue model approach used here is likely to provide an important methodology for assessing the risks for skin exposure to AgNW from consumer products.http://www.mdpi.com/2079-4991/8/4/232silver nanowirescytotoxicityskin irritation in vitroprimary keratinocytes3D reconstructed epidermis model |
| spellingShingle | Sylvia G. Lehmann Benjamin Gilbert Thierry GG Maffeis Alexei Grichine Isabelle Pignot-Paintrand Simon Clavaguera Walid Rachidi Michel Seve Laurent Charlet In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models Nanomaterials silver nanowires cytotoxicity skin irritation in vitro primary keratinocytes 3D reconstructed epidermis model |
| title | In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models |
| title_full | In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models |
| title_fullStr | In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models |
| title_full_unstemmed | In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models |
| title_short | In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models |
| title_sort | in vitro dermal safety assessment of silver nanowires after acute exposure tissue vs cell models |
| topic | silver nanowires cytotoxicity skin irritation in vitro primary keratinocytes 3D reconstructed epidermis model |
| url | http://www.mdpi.com/2079-4991/8/4/232 |
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