Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3
Background: Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration. Methods: Polycaprolactone/gelatin-based nanocomposite scaffolds containing nano-hydroxyapatite and vitamin D3 was fabri...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2017-05-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/7625 |
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| author | Mansoureh Sattary Mohamamd Rafienia Mohammad Taghi Khorasani Hossein Salehi-Rozve |
| author_facet | Mansoureh Sattary Mohamamd Rafienia Mohammad Taghi Khorasani Hossein Salehi-Rozve |
| author_sort | Mansoureh Sattary |
| collection | DOAJ |
| description | Background: Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration.
Methods: Polycaprolactone/gelatin-based nanocomposite scaffolds containing nano-hydroxyapatite and vitamin D3 was fabricated using the electrospinning method. To obtain suitable properties, solution and process parameters were optimized. Fiber morphology and MG-63 cells were cultured; chemical interactions between molecules forming scaffold and the amount of mineral deposition were determined via scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and alizarin red staining, respectively.
Findings: The mean diameter of PCL/Gel/nHA/Vit D3 nanofibers was about 531 nm. The culture of osteoblast cells on the scaffolds showed that the addition of Vit D3 to PCL/Gel/nHA scaffold caused further attachment and proliferation of the cells. In addition, stained mineral deposits scaffolds with alizarin red staining showed that the amount of mineralized deposits was significantly higher in PCL/Gel/nHA/Vit D3 scaffold than other scaffolds (P < 0.05).
Conclusion: Superior properties of nano-hydroxyapatite and vitamin D3 blended in polycaprolactone/gelatin-based scaffold were confirmed. The observations also revealed that the composite scaffold could be a good candidate for bone tissue engineering. |
| first_indexed | 2024-03-12T09:22:29Z |
| format | Article |
| id | doaj.art-b38ee62ef4ad4ea0a9b77d4149d1b1d5 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:22:29Z |
| publishDate | 2017-05-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-b38ee62ef4ad4ea0a9b77d4149d1b1d52023-09-02T14:27:21ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2017-05-01354253873922507Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3Mansoureh Sattary0Mohamamd Rafienia1Mohammad Taghi Khorasani2Hossein Salehi-Rozve3PhD Student, Department of Biomedical Engineering, School of Engineering, Science and Research Branch, Islamic Azad University, Tehran, IranAssociate Professor, Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Iran Polymer and Petrochemical Institute AND Department of Biomedical Engineering, School of Engineering, Science and Research Branch, Islamic Azad University, Tehran, IranAssistant Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration. Methods: Polycaprolactone/gelatin-based nanocomposite scaffolds containing nano-hydroxyapatite and vitamin D3 was fabricated using the electrospinning method. To obtain suitable properties, solution and process parameters were optimized. Fiber morphology and MG-63 cells were cultured; chemical interactions between molecules forming scaffold and the amount of mineral deposition were determined via scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and alizarin red staining, respectively. Findings: The mean diameter of PCL/Gel/nHA/Vit D3 nanofibers was about 531 nm. The culture of osteoblast cells on the scaffolds showed that the addition of Vit D3 to PCL/Gel/nHA scaffold caused further attachment and proliferation of the cells. In addition, stained mineral deposits scaffolds with alizarin red staining showed that the amount of mineralized deposits was significantly higher in PCL/Gel/nHA/Vit D3 scaffold than other scaffolds (P < 0.05). Conclusion: Superior properties of nano-hydroxyapatite and vitamin D3 blended in polycaprolactone/gelatin-based scaffold were confirmed. The observations also revealed that the composite scaffold could be a good candidate for bone tissue engineering.http://jims.mui.ac.ir/index.php/jims/article/view/7625BoneTissue engineeringNano-hydroxyapatite-collagenVitamin D3 |
| spellingShingle | Mansoureh Sattary Mohamamd Rafienia Mohammad Taghi Khorasani Hossein Salehi-Rozve Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3 مجله دانشکده پزشکی اصفهان Bone Tissue engineering Nano-hydroxyapatite-collagen Vitamin D3 |
| title | Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3 |
| title_full | Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3 |
| title_fullStr | Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3 |
| title_full_unstemmed | Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3 |
| title_short | Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3 |
| title_sort | study of cell behavior of the electrospun polycaprolactone gelatin scaffold containing nano hydroxyapatite and vitamin d3 |
| topic | Bone Tissue engineering Nano-hydroxyapatite-collagen Vitamin D3 |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/7625 |
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