Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosa
Background: Pseudomonas aeruginosa is an opportunistic bacterium that causes serious hospital-acquired infections. To assess the risk of clinically isolated P. aeruginosa to human health, we analyzed the resistance and virulence mechanisms of a collection of clinical isolates. Methods: This was a re...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Journal of Infection and Public Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034123004483 |
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| author | Reem S. Almaghrabi Guerrino Macori Fionn Sheridan Siobhan C. McCarthy Alexander Floss-Jones Séamus Fanning Sahar Althawadi Maysoon Mutabagani Abdulaziz Binsaslloum Mai Alrasheed Abdullah Almohaizeie Batol Allehyani Alnajla Alghofaili Marie F. Bohol Ahmed A. Al-Qahtani |
| author_facet | Reem S. Almaghrabi Guerrino Macori Fionn Sheridan Siobhan C. McCarthy Alexander Floss-Jones Séamus Fanning Sahar Althawadi Maysoon Mutabagani Abdulaziz Binsaslloum Mai Alrasheed Abdullah Almohaizeie Batol Allehyani Alnajla Alghofaili Marie F. Bohol Ahmed A. Al-Qahtani |
| author_sort | Reem S. Almaghrabi |
| collection | DOAJ |
| description | Background: Pseudomonas aeruginosa is an opportunistic bacterium that causes serious hospital-acquired infections. To assess the risk of clinically isolated P. aeruginosa to human health, we analyzed the resistance and virulence mechanisms of a collection of clinical isolates. Methods: This was a retrospective study in which P. aeruginosa isolates collected from January 1, 2018 to August 31, 2019 were analyzed using phenotypic and whole-genome sequencing (WGS) methods. The analysis included 48 clinical samples. Median patient age was 54.0 (29.5) years, and 58.3% of patients were women. Data from the microbiology laboratory database were reviewed to identify P. aeruginosa isolates. All unique isolates available for further testing were included, and related clinical data were collected. Infections were defined as hospital acquired if the index culture was obtained at least 48 h after hospitalization. Results: High-risk P. aeruginosa clones, including sequence types (STs) ST235 and ST111, were identified, in addition to 12 new STs. The isolates showed varying degrees of biofilm formation ability when evaluated at room temperature, along with reduced metabolic activity, as measured by metabolic staining, suggesting their ability to evade antimicrobial therapy. Most isolates (77.1%) were multidrug resistant (MDR), with the highest resistance and susceptibility rates to beta-lactams and colistimethate sodium, respectively. Conclusions: The MDR phenotypes of the examined isolates can be explained by the high prevalence of efflux-mediated resistance- and hydrolytic enzyme-encoding genes. These isolates had high cytotoxic potential, as indicated by the detection of toxin production-related genes. |
| first_indexed | 2024-03-08T12:47:38Z |
| format | Article |
| id | doaj.art-b38f986c54174d96ad47837ca817f0c5 |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-03-08T12:47:38Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-b38f986c54174d96ad47837ca817f0c52024-01-21T05:06:31ZengElsevierJournal of Infection and Public Health1876-03412024-02-01172299307Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosaReem S. Almaghrabi0Guerrino Macori1Fionn Sheridan2Siobhan C. McCarthy3Alexander Floss-Jones4Séamus Fanning5Sahar Althawadi6Maysoon Mutabagani7Abdulaziz Binsaslloum8Mai Alrasheed9Abdullah Almohaizeie10Batol Allehyani11Alnajla Alghofaili12Marie F. Bohol13Ahmed A. Al-Qahtani14Section of Transplant Infectious Diseases, Organ Transplant Center of Excellence, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaUCD Centre for Food Safety, Belfield, Dublin, IrelandUCD Centre for Food Safety, Belfield, Dublin, IrelandUCD Centre for Food Safety, Belfield, Dublin, IrelandUCD Centre for Food Safety, Belfield, Dublin, IrelandUCD Centre for Food Safety, Belfield, Dublin, IrelandSection of Microbiology Laboratory, Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaSection of Microbiology Laboratory, Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaSection of Microbiology Laboratory, Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaSection of Microbiology Laboratory, Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaPharmaceutical Care Division, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaPharmaceutical Care Division, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaPharmaceutical Care Division, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaDepartment of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaDepartment of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia; Department of Microbiology and Immunology, College of Medicine, Alfaisal University, Riyadh, Saudi Arabia; Corresponding author at: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.Background: Pseudomonas aeruginosa is an opportunistic bacterium that causes serious hospital-acquired infections. To assess the risk of clinically isolated P. aeruginosa to human health, we analyzed the resistance and virulence mechanisms of a collection of clinical isolates. Methods: This was a retrospective study in which P. aeruginosa isolates collected from January 1, 2018 to August 31, 2019 were analyzed using phenotypic and whole-genome sequencing (WGS) methods. The analysis included 48 clinical samples. Median patient age was 54.0 (29.5) years, and 58.3% of patients were women. Data from the microbiology laboratory database were reviewed to identify P. aeruginosa isolates. All unique isolates available for further testing were included, and related clinical data were collected. Infections were defined as hospital acquired if the index culture was obtained at least 48 h after hospitalization. Results: High-risk P. aeruginosa clones, including sequence types (STs) ST235 and ST111, were identified, in addition to 12 new STs. The isolates showed varying degrees of biofilm formation ability when evaluated at room temperature, along with reduced metabolic activity, as measured by metabolic staining, suggesting their ability to evade antimicrobial therapy. Most isolates (77.1%) were multidrug resistant (MDR), with the highest resistance and susceptibility rates to beta-lactams and colistimethate sodium, respectively. Conclusions: The MDR phenotypes of the examined isolates can be explained by the high prevalence of efflux-mediated resistance- and hydrolytic enzyme-encoding genes. These isolates had high cytotoxic potential, as indicated by the detection of toxin production-related genes.http://www.sciencedirect.com/science/article/pii/S1876034123004483GenomePseudomonas aeruginosaAntimicrobial resistance |
| spellingShingle | Reem S. Almaghrabi Guerrino Macori Fionn Sheridan Siobhan C. McCarthy Alexander Floss-Jones Séamus Fanning Sahar Althawadi Maysoon Mutabagani Abdulaziz Binsaslloum Mai Alrasheed Abdullah Almohaizeie Batol Allehyani Alnajla Alghofaili Marie F. Bohol Ahmed A. Al-Qahtani Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosa Journal of Infection and Public Health Genome Pseudomonas aeruginosa Antimicrobial resistance |
| title | Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosa |
| title_full | Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosa |
| title_fullStr | Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosa |
| title_full_unstemmed | Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosa |
| title_short | Whole genome sequencing of resistance and virulence genes in multi-drug resistant Pseudomonas aeruginosa |
| title_sort | whole genome sequencing of resistance and virulence genes in multi drug resistant pseudomonas aeruginosa |
| topic | Genome Pseudomonas aeruginosa Antimicrobial resistance |
| url | http://www.sciencedirect.com/science/article/pii/S1876034123004483 |
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