Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
Sperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2073-4409/12/22/2585 |
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| author | Ryota Tachibana Hiroki Takeuchi Kento Yoshikawa-Terada Tadashi Maezawa Mikiko Nishioka Erina Takayama Hiroaki Tanaka Kayo Tanaka Suong-hyu Hyon Yuki Gen Eiji Kondo Tomoaki Ikeda |
| author_facet | Ryota Tachibana Hiroki Takeuchi Kento Yoshikawa-Terada Tadashi Maezawa Mikiko Nishioka Erina Takayama Hiroaki Tanaka Kayo Tanaka Suong-hyu Hyon Yuki Gen Eiji Kondo Tomoaki Ikeda |
| author_sort | Ryota Tachibana |
| collection | DOAJ |
| description | Sperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced SDF after freeze-thawing compared with clinically popular cryopreservation reagents containing human serum albumin. However, it is unclear whether CPLL reduces SDF, as it differed from the constituents of the commercial cryopreservation reagents used for comparison. Therefore, here, we examined whether CPLL reduces the SDF of human sperm and evaluated reactive oxygen species (ROS) levels and lipid peroxidation (LPO), which are the causes of SDF; mitochondrial injury, ROS production; and impaired sperm motility. Furthermore, optimal antioxidants and their concentrations that could further enhance the reduction in SDF were determined for future clinical application in ART and underwent the same functional evaluations. CPLL can reduce SDF via inhibition of intracytoplasmic ROS and LPO. Furthermore, the addition of 0.1 mM resveratrol avoided the enhancement of SDF, which potentially affects mitochondrial and cytoplasmic ROS and LPO. This novel human sperm cryopreservation reagent containing CPLL and resveratrol has the potential to improve treatment outcomes in ART using frozen sperm. |
| first_indexed | 2024-03-09T16:56:58Z |
| format | Article |
| id | doaj.art-b3912201c0d248069db59293d2c76cf3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T16:56:58Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b3912201c0d248069db59293d2c76cf32023-11-24T14:35:09ZengMDPI AGCells2073-44092023-11-011222258510.3390/cells12222585Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose ResveratrolRyota Tachibana0Hiroki Takeuchi1Kento Yoshikawa-Terada2Tadashi Maezawa3Mikiko Nishioka4Erina Takayama5Hiroaki Tanaka6Kayo Tanaka7Suong-hyu Hyon8Yuki Gen9Eiji Kondo10Tomoaki Ikeda11Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, JapanCenter of Advanced Reproductive Medicine, Mie University Hospital, 2-174 Edobashi, Tsu 514-8507, JapanObstetrics and Gynecology, Mie University Hospital, 2-174 Edobashi, Tsu 514-8507, JapanObstetrics and Gynecology, Mie University Hospital, 2-174 Edobashi, Tsu 514-8507, JapanBMG, Inc., 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, JapanBMG, Inc., 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, JapanSperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced SDF after freeze-thawing compared with clinically popular cryopreservation reagents containing human serum albumin. However, it is unclear whether CPLL reduces SDF, as it differed from the constituents of the commercial cryopreservation reagents used for comparison. Therefore, here, we examined whether CPLL reduces the SDF of human sperm and evaluated reactive oxygen species (ROS) levels and lipid peroxidation (LPO), which are the causes of SDF; mitochondrial injury, ROS production; and impaired sperm motility. Furthermore, optimal antioxidants and their concentrations that could further enhance the reduction in SDF were determined for future clinical application in ART and underwent the same functional evaluations. CPLL can reduce SDF via inhibition of intracytoplasmic ROS and LPO. Furthermore, the addition of 0.1 mM resveratrol avoided the enhancement of SDF, which potentially affects mitochondrial and cytoplasmic ROS and LPO. This novel human sperm cryopreservation reagent containing CPLL and resveratrol has the potential to improve treatment outcomes in ART using frozen sperm.https://www.mdpi.com/2073-4409/12/22/2585carboxylated poly-L-lysinecryopreservationreactive oxygen speciesspermsperm DNA fragmentationantioxidant |
| spellingShingle | Ryota Tachibana Hiroki Takeuchi Kento Yoshikawa-Terada Tadashi Maezawa Mikiko Nishioka Erina Takayama Hiroaki Tanaka Kayo Tanaka Suong-hyu Hyon Yuki Gen Eiji Kondo Tomoaki Ikeda Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol Cells carboxylated poly-L-lysine cryopreservation reactive oxygen species sperm sperm DNA fragmentation antioxidant |
| title | Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol |
| title_full | Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol |
| title_fullStr | Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol |
| title_full_unstemmed | Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol |
| title_short | Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol |
| title_sort | carboxylated poly l lysine potentially reduces human sperm dna fragmentation after freeze thawing and its function is enhanced by low dose resveratrol |
| topic | carboxylated poly-L-lysine cryopreservation reactive oxygen species sperm sperm DNA fragmentation antioxidant |
| url | https://www.mdpi.com/2073-4409/12/22/2585 |
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