Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical Approach
<i>Background and Objectives</i>: MicroRNAs are short noncoding RNAs that play an essential role in controlling gene expression at the posttranscriptional level. They can serve as biomarkers in the management of obesity. Circulating miRNAs levels change with exercise, impacting various p...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/60/3/459 |
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| author | Petricia Hillari Raj Prasanth Subramanian Mohanraj Nehru Saravanan Ayyavoo Nachal Annamalai Venkataraman Prabhu |
| author_facet | Petricia Hillari Raj Prasanth Subramanian Mohanraj Nehru Saravanan Ayyavoo Nachal Annamalai Venkataraman Prabhu |
| author_sort | Petricia Hillari Raj |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: MicroRNAs are short noncoding RNAs that play an essential role in controlling gene expression at the posttranscriptional level. They can serve as biomarkers in the management of obesity. Circulating miRNAs levels change with exercise, impacting various physiological and biological systems, including structural and functional changes. <i>Aim</i>: The purpose of this study is to evaluate the levels of miRNAs 423-5p and 128-1 in young adolescents with obesity before and after an aerobic exercise programme. We also analyse the relationship between those microRNAs and obesity-related parameters in response to aerobic exercise training. <i>Materials and Methods</i>: A total of 64 adolescent individuals (32 individuals with obesity and 32 healthy individuals) were enrolled in the study to participate in a 6-month aerobic exercise programme. Anthropometric measurements, biochemical parameters and blood samples were collected from all the participants prior to exercise training and after the 6-month programme. Gene expression analysis of the study participants was performed using quantitative real-time PCR. <i>Results</i>: Expression levels of circulating microRNAs 423-5p (<i>p</i> < 0.01) and 128-1 (<i>p</i> < 0.01) differed significantly before and after exercise in the study population. Circulating miRNA 423-5p increased and correlated significantly with BMI while circulating miRNA 128-1 decreased and also significantly correlated with BMI after the 6-month aerobic exercise programme. Logistic regression analysis shows that the elevation in miRNAs expression levels has a strong significant association with the increased levels of the cytokines IL-6 and TNF-α (<i>p</i> < 0.05). <i>Conclusions</i>: Obesity leads to alterations in the expressions of miRNA 423-5p and miRNA 128-1. The significant changes observed after an aerobic exercise programme demonstrate the potential of these miRNAs as diagnostic and prognostic biomarkers for obesity. |
| first_indexed | 2024-04-24T18:01:55Z |
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| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-04-24T18:01:55Z |
| publishDate | 2024-03-01 |
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| record_format | Article |
| series | Medicina |
| spelling | doaj.art-b391764763794098aa9d73c5961ea9592024-03-27T13:53:43ZengMDPI AGMedicina1010-660X1648-91442024-03-0160345910.3390/medicina60030459Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical ApproachPetricia Hillari Raj0Prasanth Subramanian1Mohanraj Nehru2Saravanan Ayyavoo3Nachal Annamalai4Venkataraman Prabhu5Department of Physiology, Trichy SRM Medical College Hospital & Research Centre, Irungalur, Trichy 621105, Tamil Nadu, IndiaDepartment of Medical Research, SRM Medical College Hospital & Research Centre, Kattankulathur, Chennai 603203, Tamil Nadu, IndiaDepartment of Medical Research, SRM Medical College Hospital & Research Centre, Kattankulathur, Chennai 603203, Tamil Nadu, IndiaDepartment of Physiology, SRM Medical College Hospital & Research Centre, Kattankulathur, Chennai 603203, Tamil Nadu, IndiaDepartment of Physiology, Trichy SRM Medical College Hospital & Research Centre, Irungalur, Trichy 621105, Tamil Nadu, IndiaDepartment of Medical Research, SRM Medical College Hospital & Research Centre, Kattankulathur, Chennai 603203, Tamil Nadu, India<i>Background and Objectives</i>: MicroRNAs are short noncoding RNAs that play an essential role in controlling gene expression at the posttranscriptional level. They can serve as biomarkers in the management of obesity. Circulating miRNAs levels change with exercise, impacting various physiological and biological systems, including structural and functional changes. <i>Aim</i>: The purpose of this study is to evaluate the levels of miRNAs 423-5p and 128-1 in young adolescents with obesity before and after an aerobic exercise programme. We also analyse the relationship between those microRNAs and obesity-related parameters in response to aerobic exercise training. <i>Materials and Methods</i>: A total of 64 adolescent individuals (32 individuals with obesity and 32 healthy individuals) were enrolled in the study to participate in a 6-month aerobic exercise programme. Anthropometric measurements, biochemical parameters and blood samples were collected from all the participants prior to exercise training and after the 6-month programme. Gene expression analysis of the study participants was performed using quantitative real-time PCR. <i>Results</i>: Expression levels of circulating microRNAs 423-5p (<i>p</i> < 0.01) and 128-1 (<i>p</i> < 0.01) differed significantly before and after exercise in the study population. Circulating miRNA 423-5p increased and correlated significantly with BMI while circulating miRNA 128-1 decreased and also significantly correlated with BMI after the 6-month aerobic exercise programme. Logistic regression analysis shows that the elevation in miRNAs expression levels has a strong significant association with the increased levels of the cytokines IL-6 and TNF-α (<i>p</i> < 0.05). <i>Conclusions</i>: Obesity leads to alterations in the expressions of miRNA 423-5p and miRNA 128-1. The significant changes observed after an aerobic exercise programme demonstrate the potential of these miRNAs as diagnostic and prognostic biomarkers for obesity.https://www.mdpi.com/1648-9144/60/3/459obesitybiomarkerscirculating miRNAsaerobic exercise |
| spellingShingle | Petricia Hillari Raj Prasanth Subramanian Mohanraj Nehru Saravanan Ayyavoo Nachal Annamalai Venkataraman Prabhu Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical Approach Medicina obesity biomarkers circulating miRNAs aerobic exercise |
| title | Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical Approach |
| title_full | Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical Approach |
| title_fullStr | Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical Approach |
| title_full_unstemmed | Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical Approach |
| title_short | Therapeutic Impact of Aerobic Exercise on Adolescents with Obesity and Its Association with Expression of miRNAs and Cytokines: A Clinical Approach |
| title_sort | therapeutic impact of aerobic exercise on adolescents with obesity and its association with expression of mirnas and cytokines a clinical approach |
| topic | obesity biomarkers circulating miRNAs aerobic exercise |
| url | https://www.mdpi.com/1648-9144/60/3/459 |
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