<i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus
In England, <i>Cryphonectria parasitica</i> was detected for the first time in 2011 in a nursery and in 2016 in the wider environment. Surveys between 2017 and 2020 identified the disease at different sites in Berkshire, Buckinghamshire, Cornwall, Derbyshire, Devon, Dorset, London, West...
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MDPI AG
2023-10-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/10/1036 |
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| author | Pedro Romon-Ochoa Pankajini Samal Caroline Gorton Alex Lewis Ruth Chitty Amy Eacock Elzbieta Krzywinska Michael Crampton Ana Pérez-Sierra Mick Biddle Ben Jones Lisa Ward |
| author_facet | Pedro Romon-Ochoa Pankajini Samal Caroline Gorton Alex Lewis Ruth Chitty Amy Eacock Elzbieta Krzywinska Michael Crampton Ana Pérez-Sierra Mick Biddle Ben Jones Lisa Ward |
| author_sort | Pedro Romon-Ochoa |
| collection | DOAJ |
| description | In England, <i>Cryphonectria parasitica</i> was detected for the first time in 2011 in a nursery and in 2016 in the wider environment. Surveys between 2017 and 2020 identified the disease at different sites in Berkshire, Buckinghamshire, Cornwall, Derbyshire, Devon, Dorset, London, West Sussex, and the island of Jersey, while the present study comprises the results of the 2020–2023 survey with findings in Derbyshire, Devon, Kent, Nottinghamshire, Herefordshire, Leicestershire, London, West Sussex, and the islands of Jersey and Guernsey. A total of 226 suspected samples were collected from 72 surveyed sites, as far north as Edinburgh and as far west as Plymouth (both of which were negative), and 112 samples tested positive by real-time PCR and isolation from 35 sites. The 112 isolates were tested for the vegetative compatibility group (VCG), mating type, and Cryphonectria hypovirus 1 (CHV1). Twelve VCGs were identified, with two of them (EU-5 and EU-22) being the first records in the UK. Both mating types were present (37% MAT-1 and 63% MAT-2), but only one mating type was present per site and VCG, and perithecia were never observed. Cryphonectria hypovirus 1 (CHV1), consistently subtype-I haplotype E-5, was detected in three isolates at a low concentration (5.9, 21.1, and 33.0 ng/µL) from locations in London, Nottinghamshire, and Devon. |
| first_indexed | 2024-03-10T21:08:10Z |
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| id | doaj.art-b394d55d5a634974aea3ce2c310b3f85 |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T21:08:10Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-b394d55d5a634974aea3ce2c310b3f852023-11-19T17:01:21ZengMDPI AGJournal of Fungi2309-608X2023-10-01910103610.3390/jof9101036<i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 MycovirusPedro Romon-Ochoa0Pankajini Samal1Caroline Gorton2Alex Lewis3Ruth Chitty4Amy Eacock5Elzbieta Krzywinska6Michael Crampton7Ana Pérez-Sierra8Mick Biddle9Ben Jones10Lisa Ward11Forest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKForestry Commission, 620 Bristol Business Park, Bristol BS16 1EJ, UKForest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UKIn England, <i>Cryphonectria parasitica</i> was detected for the first time in 2011 in a nursery and in 2016 in the wider environment. Surveys between 2017 and 2020 identified the disease at different sites in Berkshire, Buckinghamshire, Cornwall, Derbyshire, Devon, Dorset, London, West Sussex, and the island of Jersey, while the present study comprises the results of the 2020–2023 survey with findings in Derbyshire, Devon, Kent, Nottinghamshire, Herefordshire, Leicestershire, London, West Sussex, and the islands of Jersey and Guernsey. A total of 226 suspected samples were collected from 72 surveyed sites, as far north as Edinburgh and as far west as Plymouth (both of which were negative), and 112 samples tested positive by real-time PCR and isolation from 35 sites. The 112 isolates were tested for the vegetative compatibility group (VCG), mating type, and Cryphonectria hypovirus 1 (CHV1). Twelve VCGs were identified, with two of them (EU-5 and EU-22) being the first records in the UK. Both mating types were present (37% MAT-1 and 63% MAT-2), but only one mating type was present per site and VCG, and perithecia were never observed. Cryphonectria hypovirus 1 (CHV1), consistently subtype-I haplotype E-5, was detected in three isolates at a low concentration (5.9, 21.1, and 33.0 ng/µL) from locations in London, Nottinghamshire, and Devon.https://www.mdpi.com/2309-608X/9/10/1036chestnut blightdetectionhypovirusmating typesvegetative compatibility groups |
| spellingShingle | Pedro Romon-Ochoa Pankajini Samal Caroline Gorton Alex Lewis Ruth Chitty Amy Eacock Elzbieta Krzywinska Michael Crampton Ana Pérez-Sierra Mick Biddle Ben Jones Lisa Ward <i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus Journal of Fungi chestnut blight detection hypovirus mating types vegetative compatibility groups |
| title | <i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus |
| title_full | <i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus |
| title_fullStr | <i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus |
| title_full_unstemmed | <i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus |
| title_short | <i>Cryphonectria parasitica</i> Detections in England, Jersey, and Guernsey during 2020–2023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus |
| title_sort | i cryphonectria parasitica i detections in england jersey and guernsey during 2020 2023 reveal newly affected areas and infections by the chv1 mycovirus |
| topic | chestnut blight detection hypovirus mating types vegetative compatibility groups |
| url | https://www.mdpi.com/2309-608X/9/10/1036 |
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