Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications
Human endogenous retroviruses (HERVs) have been studied and proposed as relevant cofactors in several autoimmune diseases, including type 1 diabetes (T1D), though with controversial results and no study at disease onset. In order to gather further information on the potential role of HERVs in the de...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-07-01
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| Series: | Autoimmunity |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08916934.2020.1777281 |
| _version_ | 1827817448782430208 |
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| author | Pier-Angelo Tovo Ivana Rabbone Davide Tinti Ilaria Galliano Michela Trada Valentina Daprà Franco Cerutti Massimiliano Bergallo |
| author_facet | Pier-Angelo Tovo Ivana Rabbone Davide Tinti Ilaria Galliano Michela Trada Valentina Daprà Franco Cerutti Massimiliano Bergallo |
| author_sort | Pier-Angelo Tovo |
| collection | DOAJ |
| description | Human endogenous retroviruses (HERVs) have been studied and proposed as relevant cofactors in several autoimmune diseases, including type 1 diabetes (T1D), though with controversial results and no study at disease onset. In order to gather further information on the potential role of HERVs in the development of T1D we assessed the transcription levels of pol genes of HERV-H, HERV-K, and HERV-W in peripheral leucocytes from 37 children and adolescents with new-onset T1D and 50 age-matched control subjects. A PCR real time Taqman amplification assay was used to evaluate HERV transcripts with normalisation of the results to glyceraldehyde-3-phosphate dehydrogenase. The expression levels of HERV-H-pol gene and HERV-W-pol gene were significantly higher in diabetic patients than in control subjects. Conversely, no significant difference emerged in the expression levels of HERV-K-pol gene between diabetic patients and controls. The activation of HERV-H and HERV-W in new-onset T1D suggests their importance in the pathogenesis of the disease and supports targeted therapeutic attempts to hinder their activation. |
| first_indexed | 2024-03-12T00:34:23Z |
| format | Article |
| id | doaj.art-b396796fc54648a0ae79bd261fa81fd5 |
| institution | Directory Open Access Journal |
| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-12T00:34:23Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj.art-b396796fc54648a0ae79bd261fa81fd52023-09-15T10:01:09ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2020-07-0153528328810.1080/08916934.2020.17772811777281Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implicationsPier-Angelo Tovo0Ivana Rabbone1Davide Tinti2Ilaria Galliano3Michela Trada4Valentina Daprà5Franco Cerutti6Massimiliano Bergallo7University of TurinUniversity of TurinUniversity of TurinUniversity of TurinUniversity of TurinUniversity of TurinUniversity of TurinUniversity of TurinHuman endogenous retroviruses (HERVs) have been studied and proposed as relevant cofactors in several autoimmune diseases, including type 1 diabetes (T1D), though with controversial results and no study at disease onset. In order to gather further information on the potential role of HERVs in the development of T1D we assessed the transcription levels of pol genes of HERV-H, HERV-K, and HERV-W in peripheral leucocytes from 37 children and adolescents with new-onset T1D and 50 age-matched control subjects. A PCR real time Taqman amplification assay was used to evaluate HERV transcripts with normalisation of the results to glyceraldehyde-3-phosphate dehydrogenase. The expression levels of HERV-H-pol gene and HERV-W-pol gene were significantly higher in diabetic patients than in control subjects. Conversely, no significant difference emerged in the expression levels of HERV-K-pol gene between diabetic patients and controls. The activation of HERV-H and HERV-W in new-onset T1D suggests their importance in the pathogenesis of the disease and supports targeted therapeutic attempts to hinder their activation.http://dx.doi.org/10.1080/08916934.2020.1777281human endogenous retrovirusesnew-onset type 1 diabeteschildrenadolescents |
| spellingShingle | Pier-Angelo Tovo Ivana Rabbone Davide Tinti Ilaria Galliano Michela Trada Valentina Daprà Franco Cerutti Massimiliano Bergallo Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications Autoimmunity human endogenous retroviruses new-onset type 1 diabetes children adolescents |
| title | Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications |
| title_full | Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications |
| title_fullStr | Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications |
| title_full_unstemmed | Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications |
| title_short | Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications |
| title_sort | enhanced expression of human endogenous retroviruses in new onset type 1 diabetes potential pathogenetic and therapeutic implications |
| topic | human endogenous retroviruses new-onset type 1 diabetes children adolescents |
| url | http://dx.doi.org/10.1080/08916934.2020.1777281 |
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