Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane
Drought limits crop productivity, especially of sugarcane, which is predominantly grown in the subtropical parts of China. Soil microbes perform a wide range of functions that are important for plant productivity and responses to drought stress, and fungi play an important role in plant–soil interac...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.562775/full |
| _version_ | 1798045870524989440 |
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| author | Xiaowen Zhao Xiaowen Zhao Xiaowen Zhao Qi Liu Qi Liu Qi Liu Sasa Xie Sasa Xie Sasa Xie Yuke Jiang Yuke Jiang Yuke Jiang Huichun Yang Huichun Yang Huichun Yang Ziting Wang Ziting Wang Ziting Wang Muqing Zhang Muqing Zhang Muqing Zhang |
| author_facet | Xiaowen Zhao Xiaowen Zhao Xiaowen Zhao Qi Liu Qi Liu Qi Liu Sasa Xie Sasa Xie Sasa Xie Yuke Jiang Yuke Jiang Yuke Jiang Huichun Yang Huichun Yang Huichun Yang Ziting Wang Ziting Wang Ziting Wang Muqing Zhang Muqing Zhang Muqing Zhang |
| author_sort | Xiaowen Zhao |
| collection | DOAJ |
| description | Drought limits crop productivity, especially of sugarcane, which is predominantly grown in the subtropical parts of China. Soil microbes perform a wide range of functions that are important for plant productivity and responses to drought stress, and fungi play an important role in plant–soil interactions. The Ea-DREB2B gene of sugarcane, Saccharum arundinaceum, is involved in regulating the response to drought stress. In this study, fungal communities of the transgenic (TG) sugarcane variety GN18, harboring the drought-tolerant gene Ea-DREB2B and its corresponding non-TG wild-type (WT) variety, FN95-1702, were investigated in three soil compartments (rhizoplane, rhizosphere, and bulk soil) by assessing the internal transcribed spacer region using Illumina MiSeq. As the soil microbial community is also affected by various environmental factors, such as pH, carbon availability, and soil moisture, we determined the total carbon (TC), total nitrogen (TN), and total phosphorus (TP) contents in the rhizoplane, rhizosphere, and bulk soil compartments to explore the associations between soil fungal communities and host plant characteristics. The differences between the soil fungal communities of TG and WT plants were detected. The alpha diversity of TG fungal communities was more correlated to environmental factors than the beta diversity. The abundance of operational taxonomic units (OTUs) enriched in TG root-related area was far more than that in the root-related area of WT plants. Thereinto, more saprotrophs were enriched in the TG root-related area, indicating altered niches of fungal guilds around TG roots. These results revealed that host plant genotype did play a key role for strengthening plant–fungi interaction and enhancing beneficial fungal function in the root-related area (rhizoplane and rhizosphere) of TG sugarcane in order to respond to drought stress. |
| first_indexed | 2024-04-11T23:29:23Z |
| format | Article |
| id | doaj.art-b39b6f9fc7514e37be68861fd37106bd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T23:29:23Z |
| publishDate | 2020-09-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-b39b6f9fc7514e37be68861fd37106bd2022-12-22T03:57:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-09-011110.3389/fmicb.2020.562775562775Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic SugarcaneXiaowen Zhao0Xiaowen Zhao1Xiaowen Zhao2Qi Liu3Qi Liu4Qi Liu5Sasa Xie6Sasa Xie7Sasa Xie8Yuke Jiang9Yuke Jiang10Yuke Jiang11Huichun Yang12Huichun Yang13Huichun Yang14Ziting Wang15Ziting Wang16Ziting Wang17Muqing Zhang18Muqing Zhang19Muqing Zhang20Guangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaDrought limits crop productivity, especially of sugarcane, which is predominantly grown in the subtropical parts of China. Soil microbes perform a wide range of functions that are important for plant productivity and responses to drought stress, and fungi play an important role in plant–soil interactions. The Ea-DREB2B gene of sugarcane, Saccharum arundinaceum, is involved in regulating the response to drought stress. In this study, fungal communities of the transgenic (TG) sugarcane variety GN18, harboring the drought-tolerant gene Ea-DREB2B and its corresponding non-TG wild-type (WT) variety, FN95-1702, were investigated in three soil compartments (rhizoplane, rhizosphere, and bulk soil) by assessing the internal transcribed spacer region using Illumina MiSeq. As the soil microbial community is also affected by various environmental factors, such as pH, carbon availability, and soil moisture, we determined the total carbon (TC), total nitrogen (TN), and total phosphorus (TP) contents in the rhizoplane, rhizosphere, and bulk soil compartments to explore the associations between soil fungal communities and host plant characteristics. The differences between the soil fungal communities of TG and WT plants were detected. The alpha diversity of TG fungal communities was more correlated to environmental factors than the beta diversity. The abundance of operational taxonomic units (OTUs) enriched in TG root-related area was far more than that in the root-related area of WT plants. Thereinto, more saprotrophs were enriched in the TG root-related area, indicating altered niches of fungal guilds around TG roots. These results revealed that host plant genotype did play a key role for strengthening plant–fungi interaction and enhancing beneficial fungal function in the root-related area (rhizoplane and rhizosphere) of TG sugarcane in order to respond to drought stress.https://www.frontiersin.org/article/10.3389/fmicb.2020.562775/fulldrought resistanceEa-DREB2Btransgenic sugarcanefungal communityenvironmental factor |
| spellingShingle | Xiaowen Zhao Xiaowen Zhao Xiaowen Zhao Qi Liu Qi Liu Qi Liu Sasa Xie Sasa Xie Sasa Xie Yuke Jiang Yuke Jiang Yuke Jiang Huichun Yang Huichun Yang Huichun Yang Ziting Wang Ziting Wang Ziting Wang Muqing Zhang Muqing Zhang Muqing Zhang Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane Frontiers in Microbiology drought resistance Ea-DREB2B transgenic sugarcane fungal community environmental factor |
| title | Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane |
| title_full | Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane |
| title_fullStr | Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane |
| title_full_unstemmed | Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane |
| title_short | Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane |
| title_sort | response of soil fungal community to drought resistant ea dreb2b transgenic sugarcane |
| topic | drought resistance Ea-DREB2B transgenic sugarcane fungal community environmental factor |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.562775/full |
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