Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery
Summary The safety, quantitative method and delivery of faecal microbiota transplantation (FMT) vary a lot from different countries in practice. Recently, the improved methodology of FMT based on the automatic filtration, washing process and the related delivery was named as washed microbiota transp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14074 |
| _version_ | 1811184670043275264 |
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| author | Gaochen Lu Weihong Wang Pan Li Quan Wen Bota Cui Faming Zhang |
| author_facet | Gaochen Lu Weihong Wang Pan Li Quan Wen Bota Cui Faming Zhang |
| author_sort | Gaochen Lu |
| collection | DOAJ |
| description | Summary The safety, quantitative method and delivery of faecal microbiota transplantation (FMT) vary a lot from different countries in practice. Recently, the improved methodology of FMT based on the automatic filtration, washing process and the related delivery was named as washed microbiota transplantation (WMT). First, this study aimed to describe the methodology development of FMT from manual to washing preparation from 2012 to 2021 in China Microbiota Transplantation System (CMTS), a centralized stool bank for providing a national non‐profit service. The secondary aim is to describe donor screenings, the correlation between faecal weight and treatment doses, incidence of adverse events and delivery decision. The retrospective analysis on the prospectively recorded data was performed. Results showed that the success rate of donor screening was 3.1% (32/1036). The incidence rate of fever decreased significantly from 19.4% (6/31) in manual FMT to 2.7% (24/902) in WMT in patients with ulcerative colitis (UC), which made UC a considerable disease model to reflect the quality control of faecal microbiota preparation. We defined one treatment unit as 10 cm3 microbiota precipitation (1.0 × 1013 bacteria) based on enriched microbiota instead of rough faecal weight. For delivering microbiota, colonic transendoscopic enteral tube is a promising way especially for multiple WMTs or frequent colonic administration of drugs combined with WMT. This study should help improve the better practice of FMT for helping more patients in the future. |
| first_indexed | 2024-04-11T13:16:13Z |
| format | Article |
| id | doaj.art-b39d059a628e40b69a5bd83e2eb7d69e |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-11T13:16:13Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-b39d059a628e40b69a5bd83e2eb7d69e2022-12-22T04:22:24ZengWileyMicrobial Biotechnology1751-79152022-09-011592439244910.1111/1751-7915.14074Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and deliveryGaochen Lu0Weihong Wang1Pan Li2Quan Wen3Bota Cui4Faming Zhang5Medical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 ChinaMedical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 ChinaMedical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 ChinaMedical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 ChinaMedical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 ChinaMedical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 ChinaSummary The safety, quantitative method and delivery of faecal microbiota transplantation (FMT) vary a lot from different countries in practice. Recently, the improved methodology of FMT based on the automatic filtration, washing process and the related delivery was named as washed microbiota transplantation (WMT). First, this study aimed to describe the methodology development of FMT from manual to washing preparation from 2012 to 2021 in China Microbiota Transplantation System (CMTS), a centralized stool bank for providing a national non‐profit service. The secondary aim is to describe donor screenings, the correlation between faecal weight and treatment doses, incidence of adverse events and delivery decision. The retrospective analysis on the prospectively recorded data was performed. Results showed that the success rate of donor screening was 3.1% (32/1036). The incidence rate of fever decreased significantly from 19.4% (6/31) in manual FMT to 2.7% (24/902) in WMT in patients with ulcerative colitis (UC), which made UC a considerable disease model to reflect the quality control of faecal microbiota preparation. We defined one treatment unit as 10 cm3 microbiota precipitation (1.0 × 1013 bacteria) based on enriched microbiota instead of rough faecal weight. For delivering microbiota, colonic transendoscopic enteral tube is a promising way especially for multiple WMTs or frequent colonic administration of drugs combined with WMT. This study should help improve the better practice of FMT for helping more patients in the future.https://doi.org/10.1111/1751-7915.14074 |
| spellingShingle | Gaochen Lu Weihong Wang Pan Li Quan Wen Bota Cui Faming Zhang Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery Microbial Biotechnology |
| title | Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery |
| title_full | Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery |
| title_fullStr | Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery |
| title_full_unstemmed | Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery |
| title_short | Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery |
| title_sort | washed preparation of faecal microbiota changes the transplantation related safety quantitative method and delivery |
| url | https://doi.org/10.1111/1751-7915.14074 |
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