A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy
This study aimed to examine the efficacy of epirubicin-loaded gelatin hydrogel (EPI-H) in the treatment of superficial urothelium carcinoma. Hydrogel was prepared by Schiff base-crosslinking of gelatin with glutaraldehyde. EPI-H exhibited high entrapment efficiency (59.87% ± 0.51%). EPI-H also incre...
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MDPI AG
2016-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/6/712 |
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| author | Ching-Wen Liu Yu-Tse Wu Kai-Jen Lin Tsan-Jung Yu Yu-Liang Kuo Li-Ching Chang |
| author_facet | Ching-Wen Liu Yu-Tse Wu Kai-Jen Lin Tsan-Jung Yu Yu-Liang Kuo Li-Ching Chang |
| author_sort | Ching-Wen Liu |
| collection | DOAJ |
| description | This study aimed to examine the efficacy of epirubicin-loaded gelatin hydrogel (EPI-H) in the treatment of superficial urothelium carcinoma. Hydrogel was prepared by Schiff base-crosslinking of gelatin with glutaraldehyde. EPI-H exhibited high entrapment efficiency (59.87% ± 0.51%). EPI-H also increased epirubicin accumulation in AY-27 cells when compared with the effect of aqueous solutions of epirubicin (EPI-AQ); respective epirubicin-positive cell counts were 69.0% ± 7.6% and 38.3% ± 5.8%. EPI-H also exhibited greater cytotoxicity against AY-27 cells than that of EPI-AQ; IC50 values were 13.1 ± 1.1 and 7.5 ± 0.3 μg/mL, respectively. Cystometrograms showed that EPI-H reduced peak micturition, threshold pressures, and micturition duration, and that it increased bladder compliance more so than EPI-AQ. EPI-H enhanced epirubicin penetration into basal cells of urothelium in vivo, whereas EPI-AQ did so only to the umbrella cells. EPI-H inhibited tumor growth upon intravesical instillation to tumor-bearing bladder of F344 rats, inducing higher levels of caspase-3 expression than that observed with EPI-AQ treatment; the number of caspase-3 positive cells in treated urothelium carcinoma was 13.9% ± 4.0% (EPI-AQ) and 34.1% ± 1.0%, (EPI-H). EPI-H has value as an improved means to administer epirubicin in intravesical instillation treatments for bladder cancer. |
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| language | English |
| last_indexed | 2024-04-14T02:23:22Z |
| publishDate | 2016-06-01 |
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| series | Molecules |
| spelling | doaj.art-b3a155e4bd204d449b6863ae9bafd7a02022-12-22T02:17:57ZengMDPI AGMolecules1420-30492016-06-0121671210.3390/molecules21060712molecules21060712A Hydrogel-Based Epirubicin Delivery System for Intravesical ChemotherapyChing-Wen Liu0Yu-Tse Wu1Kai-Jen Lin2Tsan-Jung Yu3Yu-Liang Kuo4Li-Ching Chang5School of Pharmacy, Kaohsiung Medical University, No.100, Shih-Chuan 1st Road, Sanmin District, Kaohsiung 807, TaiwanSchool of Pharmacy, Kaohsiung Medical University, No.100, Shih-Chuan 1st Road, Sanmin District, Kaohsiung 807, TaiwanDepartment of Pathology, E-Da Hospital, I-Shou University, No.1, Yida Road, Yanchao District, Kaohsiung 824, TaiwanDepartment of Urology, E-Da Hospital, I-Shou University, No.1, Yida Road, Yanchao District, Kaohsiung 824, TaiwanDepartment of Medical Imaging and Radiological Sciences, Chung Shan Medical University, No.110, Sec. 1, Jianguo North Rd., South District., Taichung 402, TaiwanDepartment of Occupational Therapy, I-Shou University, No.8, Yida Road, Yanchao District, Kaohsiung 824, TaiwanThis study aimed to examine the efficacy of epirubicin-loaded gelatin hydrogel (EPI-H) in the treatment of superficial urothelium carcinoma. Hydrogel was prepared by Schiff base-crosslinking of gelatin with glutaraldehyde. EPI-H exhibited high entrapment efficiency (59.87% ± 0.51%). EPI-H also increased epirubicin accumulation in AY-27 cells when compared with the effect of aqueous solutions of epirubicin (EPI-AQ); respective epirubicin-positive cell counts were 69.0% ± 7.6% and 38.3% ± 5.8%. EPI-H also exhibited greater cytotoxicity against AY-27 cells than that of EPI-AQ; IC50 values were 13.1 ± 1.1 and 7.5 ± 0.3 μg/mL, respectively. Cystometrograms showed that EPI-H reduced peak micturition, threshold pressures, and micturition duration, and that it increased bladder compliance more so than EPI-AQ. EPI-H enhanced epirubicin penetration into basal cells of urothelium in vivo, whereas EPI-AQ did so only to the umbrella cells. EPI-H inhibited tumor growth upon intravesical instillation to tumor-bearing bladder of F344 rats, inducing higher levels of caspase-3 expression than that observed with EPI-AQ treatment; the number of caspase-3 positive cells in treated urothelium carcinoma was 13.9% ± 4.0% (EPI-AQ) and 34.1% ± 1.0%, (EPI-H). EPI-H has value as an improved means to administer epirubicin in intravesical instillation treatments for bladder cancer.http://www.mdpi.com/1420-3049/21/6/712epirubicinhydrogelintravesicalbladder cancer |
| spellingShingle | Ching-Wen Liu Yu-Tse Wu Kai-Jen Lin Tsan-Jung Yu Yu-Liang Kuo Li-Ching Chang A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy Molecules epirubicin hydrogel intravesical bladder cancer |
| title | A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy |
| title_full | A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy |
| title_fullStr | A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy |
| title_full_unstemmed | A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy |
| title_short | A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy |
| title_sort | hydrogel based epirubicin delivery system for intravesical chemotherapy |
| topic | epirubicin hydrogel intravesical bladder cancer |
| url | http://www.mdpi.com/1420-3049/21/6/712 |
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