Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i>
A newly isolated bacteriophage infecting <i>Enterococcus faecalis</i> strains has been characterized, including determination of its molecular features. This phage, named vB_EfaS-271, has been classified as a <i>Siphoviridae</i> member, according to electron microscopy charac...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/17/6345 |
| _version_ | 1797554927137980416 |
|---|---|
| author | Gracja Topka-Bielecka Sylwia Bloch Bożena Nejman-Faleńczyk Michał Grabski Agata Jurczak-Kurek Marcin Górniak Aleksandra Dydecka Agnieszka Necel Grzegorz Węgrzyn Alicja Węgrzyn |
| author_facet | Gracja Topka-Bielecka Sylwia Bloch Bożena Nejman-Faleńczyk Michał Grabski Agata Jurczak-Kurek Marcin Górniak Aleksandra Dydecka Agnieszka Necel Grzegorz Węgrzyn Alicja Węgrzyn |
| author_sort | Gracja Topka-Bielecka |
| collection | DOAJ |
| description | A newly isolated bacteriophage infecting <i>Enterococcus faecalis</i> strains has been characterized, including determination of its molecular features. This phage, named vB_EfaS-271, has been classified as a <i>Siphoviridae</i> member, according to electron microscopy characterization of the virions, composed of a 50 nm-diameter head and a long, flexible, noncontractable tail (219 × 12.5 nm). Analysis of the whole dsDNA genome of this phage showed that it consists of 40,197 bp and functional modules containing genes coding for proteins that are involved in DNA replication (including DNA polymerase/primase), morphogenesis, packaging and cell lysis. Mass spectrometry analysis allowed us to identify several phage-encoded proteins. vB_EfaS-271 reveals a relatively narrow host range, as it is able to infect only a few <i>E. faecalis</i> strains. On the other hand, it is a virulent phage (unable to lysogenize host cells), effectively and quickly destroying cultures of sensitive host bacteria, with a latent period as short as 8 min and burst size of approximately 70 phages per cell at 37 °C. This phage was also able to destroy biofilms formed by <i>E. faecalis</i>. These results contribute to our understanding of the biodiversity of bacteriophages, confirming the high variability among these viruses and indicating specific genetic and functional features of vB_EfaS-271. |
| first_indexed | 2024-03-10T16:39:03Z |
| format | Article |
| id | doaj.art-b3a1fdcea9a8430799e5754c47f38dc0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:39:03Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b3a1fdcea9a8430799e5754c47f38dc02023-11-20T12:11:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012117634510.3390/ijms21176345Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i>Gracja Topka-Bielecka0Sylwia Bloch1Bożena Nejman-Faleńczyk2Michał Grabski3Agata Jurczak-Kurek4Marcin Górniak5Aleksandra Dydecka6Agnieszka Necel7Grzegorz Węgrzyn8Alicja Węgrzyn9Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandLaboratory of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdansk, PolandDepartment of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandDepartment of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandDepartment of Molecular Evolution, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandDepartment of Molecular Evolution, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandDepartment of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandDepartment of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandDepartment of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, PolandLaboratory of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdansk, PolandA newly isolated bacteriophage infecting <i>Enterococcus faecalis</i> strains has been characterized, including determination of its molecular features. This phage, named vB_EfaS-271, has been classified as a <i>Siphoviridae</i> member, according to electron microscopy characterization of the virions, composed of a 50 nm-diameter head and a long, flexible, noncontractable tail (219 × 12.5 nm). Analysis of the whole dsDNA genome of this phage showed that it consists of 40,197 bp and functional modules containing genes coding for proteins that are involved in DNA replication (including DNA polymerase/primase), morphogenesis, packaging and cell lysis. Mass spectrometry analysis allowed us to identify several phage-encoded proteins. vB_EfaS-271 reveals a relatively narrow host range, as it is able to infect only a few <i>E. faecalis</i> strains. On the other hand, it is a virulent phage (unable to lysogenize host cells), effectively and quickly destroying cultures of sensitive host bacteria, with a latent period as short as 8 min and burst size of approximately 70 phages per cell at 37 °C. This phage was also able to destroy biofilms formed by <i>E. faecalis</i>. These results contribute to our understanding of the biodiversity of bacteriophages, confirming the high variability among these viruses and indicating specific genetic and functional features of vB_EfaS-271.https://www.mdpi.com/1422-0067/21/17/6345bacteriophage<i>Enterococcus faecalis</i>genomic analysisphage developmentbiofilm destructionbiodiversity of viruses |
| spellingShingle | Gracja Topka-Bielecka Sylwia Bloch Bożena Nejman-Faleńczyk Michał Grabski Agata Jurczak-Kurek Marcin Górniak Aleksandra Dydecka Agnieszka Necel Grzegorz Węgrzyn Alicja Węgrzyn Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i> International Journal of Molecular Sciences bacteriophage <i>Enterococcus faecalis</i> genomic analysis phage development biofilm destruction biodiversity of viruses |
| title | Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i> |
| title_full | Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i> |
| title_fullStr | Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i> |
| title_full_unstemmed | Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i> |
| title_short | Characterization of the Bacteriophage vB_EfaS-271 Infecting <i>Enterococcus faecalis</i> |
| title_sort | characterization of the bacteriophage vb efas 271 infecting i enterococcus faecalis i |
| topic | bacteriophage <i>Enterococcus faecalis</i> genomic analysis phage development biofilm destruction biodiversity of viruses |
| url | https://www.mdpi.com/1422-0067/21/17/6345 |
| work_keys_str_mv | AT gracjatopkabielecka characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT sylwiabloch characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT bozenanejmanfalenczyk characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT michałgrabski characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT agatajurczakkurek characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT marcingorniak characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT aleksandradydecka characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT agnieszkanecel characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT grzegorzwegrzyn characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi AT alicjawegrzyn characterizationofthebacteriophagevbefas271infectingienterococcusfaecalisi |