Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In Vivo
In the context of the growing prevalence of type 2 diabetes (T2DM), control of postprandial hyperglycemia is crucial for its prevention. Blood glucose levels are determined by various factors including carbohydrate hydrolyzing enzymes, the incretin system and glucose transporters. Furthermore, infla...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/6/1392 |
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| author | Kai Lüersen Alexandra Fischer Ilka Bauer Patricia Huebbe Yukiko Uekaji Keita Chikamoto Daisuke Nakata Naoto Hiramatsu Keiji Terao Gerald Rimbach |
| author_facet | Kai Lüersen Alexandra Fischer Ilka Bauer Patricia Huebbe Yukiko Uekaji Keita Chikamoto Daisuke Nakata Naoto Hiramatsu Keiji Terao Gerald Rimbach |
| author_sort | Kai Lüersen |
| collection | DOAJ |
| description | In the context of the growing prevalence of type 2 diabetes (T2DM), control of postprandial hyperglycemia is crucial for its prevention. Blood glucose levels are determined by various factors including carbohydrate hydrolyzing enzymes, the incretin system and glucose transporters. Furthermore, inflammatory markers are recognized predictors of diabetes outcome. Although there is some evidence that isoflavones may exhibit anti-diabetic properties, little is known about to what extent their corresponding hydroxylated metabolites may affect glucose metabolism. We evaluated the ability of a soy extract before (pre-) and after (post-) fermentation to counteract hyperglycemia in vitro and in <i>Drosophila melanogaster</i> in vivo. Fermentation with <i>Aspergillus</i> sp. <i>JCM22299</i> led to an enrichment of hydroxy-isoflavones (HI), including 8-hydroxygenistein, 8-hydroxyglycitein and 8-hydroxydaidzein, accompanied by an enhanced free radical scavenging activity. This HI-rich extract demonstrated inhibitory activity towards α-glucosidase and a reduction of dipeptidyl peptidase-4 enzyme activity. Both the pre- and post-fermented extracts significantly inhibited the glucose transport via sodium-dependent glucose transporter 1. Furthermore, the soy extracts reduced c-reactive protein mRNA and secreted protein levels in interleukin-stimulated Hep B3 cells. Finally, supplementation of a high-starch <i>D. melanogaster</i> diet with post-fermented HI-rich extract decreased the triacylglyceride content of female fruit flies, confirming its anti-diabetic properties in an in vivo model. |
| first_indexed | 2024-03-11T06:04:29Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T06:04:29Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b3a225a0d0e24924a0aee4fa07ebbc3f2023-11-17T13:05:09ZengMDPI AGNutrients2072-66432023-03-01156139210.3390/nu15061392Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In VivoKai Lüersen0Alexandra Fischer1Ilka Bauer2Patricia Huebbe3Yukiko Uekaji4Keita Chikamoto5Daisuke Nakata6Naoto Hiramatsu7Keiji Terao8Gerald Rimbach9Institute of Human Nutrition and Food Science, University of Kiel, 24118 Kiel, GermanyInstitute of Human Nutrition and Food Science, University of Kiel, 24118 Kiel, GermanyInstitute of Human Nutrition and Food Science, University of Kiel, 24118 Kiel, GermanyInstitute of Human Nutrition and Food Science, University of Kiel, 24118 Kiel, GermanyCycloChem Bio Co., Ltd., 7-4-5 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Hyogo, JapanCycloChem Bio Co., Ltd., 7-4-5 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Hyogo, JapanCycloChem Bio Co., Ltd., 7-4-5 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Hyogo, JapanToyo Hakko Co., Ltd., 1-39-1 Yoshikawa-cho, Obu-shi 474-0046, Aichi, JapanCycloChem Bio Co., Ltd., 7-4-5 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Hyogo, JapanInstitute of Human Nutrition and Food Science, University of Kiel, 24118 Kiel, GermanyIn the context of the growing prevalence of type 2 diabetes (T2DM), control of postprandial hyperglycemia is crucial for its prevention. Blood glucose levels are determined by various factors including carbohydrate hydrolyzing enzymes, the incretin system and glucose transporters. Furthermore, inflammatory markers are recognized predictors of diabetes outcome. Although there is some evidence that isoflavones may exhibit anti-diabetic properties, little is known about to what extent their corresponding hydroxylated metabolites may affect glucose metabolism. We evaluated the ability of a soy extract before (pre-) and after (post-) fermentation to counteract hyperglycemia in vitro and in <i>Drosophila melanogaster</i> in vivo. Fermentation with <i>Aspergillus</i> sp. <i>JCM22299</i> led to an enrichment of hydroxy-isoflavones (HI), including 8-hydroxygenistein, 8-hydroxyglycitein and 8-hydroxydaidzein, accompanied by an enhanced free radical scavenging activity. This HI-rich extract demonstrated inhibitory activity towards α-glucosidase and a reduction of dipeptidyl peptidase-4 enzyme activity. Both the pre- and post-fermented extracts significantly inhibited the glucose transport via sodium-dependent glucose transporter 1. Furthermore, the soy extracts reduced c-reactive protein mRNA and secreted protein levels in interleukin-stimulated Hep B3 cells. Finally, supplementation of a high-starch <i>D. melanogaster</i> diet with post-fermented HI-rich extract decreased the triacylglyceride content of female fruit flies, confirming its anti-diabetic properties in an in vivo model.https://www.mdpi.com/2072-6643/15/6/1392soyisoflavoneshydroxy isoflavonesbioactivityglucose metabolism |
| spellingShingle | Kai Lüersen Alexandra Fischer Ilka Bauer Patricia Huebbe Yukiko Uekaji Keita Chikamoto Daisuke Nakata Naoto Hiramatsu Keiji Terao Gerald Rimbach Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In Vivo Nutrients soy isoflavones hydroxy isoflavones bioactivity glucose metabolism |
| title | Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In Vivo |
| title_full | Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In Vivo |
| title_fullStr | Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In Vivo |
| title_full_unstemmed | Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In Vivo |
| title_short | Soy Extract, Rich in Hydroxylated Isoflavones, Exhibits Antidiabetic Properties In Vitro and in <i>Drosophila melanogaster</i> In Vivo |
| title_sort | soy extract rich in hydroxylated isoflavones exhibits antidiabetic properties in vitro and in i drosophila melanogaster i in vivo |
| topic | soy isoflavones hydroxy isoflavones bioactivity glucose metabolism |
| url | https://www.mdpi.com/2072-6643/15/6/1392 |
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