Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations
Abstract Background Microbial production of biofuels and biochemicals from renewable feedstocks has received considerable recent attention from environmental protection and energy production perspectives. Many biofuels and biochemicals are produced by fermentation under oxygen-limited conditions fol...
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | http://link.springer.com/article/10.1186/s13068-017-0867-0 |
| _version_ | 1811243523292266496 |
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| author | Yu Matsuoka Hiroyuki Kurata |
| author_facet | Yu Matsuoka Hiroyuki Kurata |
| author_sort | Yu Matsuoka |
| collection | DOAJ |
| description | Abstract Background Microbial production of biofuels and biochemicals from renewable feedstocks has received considerable recent attention from environmental protection and energy production perspectives. Many biofuels and biochemicals are produced by fermentation under oxygen-limited conditions following initiation of aerobic cultivation to enhance the cell growth rate. Thus, it is of significant interest to investigate the effect of dissolved oxygen concentration on redox regulation in Escherichia coli, a particularly popular cellular factory due to its high growth rate and well-characterized physiology. For this, the systems biology approach such as modeling is powerful for the analysis of the metabolism and for the design of microbial cellular factories. Results Here, we developed a kinetic model that describes the dynamics of fermentation by taking into account transcription factors such as ArcA/B and Fnr, respiratory chain reactions and fermentative pathways, and catabolite regulation. The hallmark of the kinetic model is its ability to predict the dynamics of metabolism at different dissolved oxygen levels and facilitate the rational design of cultivation methods. The kinetic model was verified based on the experimental data for a wild-type E. coli strain. The model reasonably predicted the metabolic characteristics and molecular mechanisms of fnr and arcA gene-knockout mutants. Moreover, an aerobic–microaerobic dual-phase cultivation method for lactate production in a pfl-knockout mutant exhibited promising yield and productivity. Conclusions It is quite important to understand metabolic regulation mechanisms from both scientific and engineering points of view. In particular, redox regulation in response to oxygen limitation is critically important in the practical production of biofuel and biochemical compounds. The developed model can thus be used as a platform for designing microbial factories to produce a variety of biofuels and biochemicals. |
| first_indexed | 2024-04-12T14:09:58Z |
| format | Article |
| id | doaj.art-b3a37c65b1d945a49f2d322f1e46b572 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T14:09:58Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-b3a37c65b1d945a49f2d322f1e46b5722022-12-22T03:29:56ZengBMCBiotechnology for Biofuels1754-68342017-07-0110111510.1186/s13068-017-0867-0Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrationsYu Matsuoka0Hiroyuki Kurata1Department of Bioscience and Bioinformatics, Kyushu Institute of TechnologyDepartment of Bioscience and Bioinformatics, Kyushu Institute of TechnologyAbstract Background Microbial production of biofuels and biochemicals from renewable feedstocks has received considerable recent attention from environmental protection and energy production perspectives. Many biofuels and biochemicals are produced by fermentation under oxygen-limited conditions following initiation of aerobic cultivation to enhance the cell growth rate. Thus, it is of significant interest to investigate the effect of dissolved oxygen concentration on redox regulation in Escherichia coli, a particularly popular cellular factory due to its high growth rate and well-characterized physiology. For this, the systems biology approach such as modeling is powerful for the analysis of the metabolism and for the design of microbial cellular factories. Results Here, we developed a kinetic model that describes the dynamics of fermentation by taking into account transcription factors such as ArcA/B and Fnr, respiratory chain reactions and fermentative pathways, and catabolite regulation. The hallmark of the kinetic model is its ability to predict the dynamics of metabolism at different dissolved oxygen levels and facilitate the rational design of cultivation methods. The kinetic model was verified based on the experimental data for a wild-type E. coli strain. The model reasonably predicted the metabolic characteristics and molecular mechanisms of fnr and arcA gene-knockout mutants. Moreover, an aerobic–microaerobic dual-phase cultivation method for lactate production in a pfl-knockout mutant exhibited promising yield and productivity. Conclusions It is quite important to understand metabolic regulation mechanisms from both scientific and engineering points of view. In particular, redox regulation in response to oxygen limitation is critically important in the practical production of biofuel and biochemical compounds. The developed model can thus be used as a platform for designing microbial factories to produce a variety of biofuels and biochemicals.http://link.springer.com/article/10.1186/s13068-017-0867-0Kinetic modelingFermentationDissolved oxygen limitationRedox regulationArcAFnr |
| spellingShingle | Yu Matsuoka Hiroyuki Kurata Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations Biotechnology for Biofuels Kinetic modeling Fermentation Dissolved oxygen limitation Redox regulation ArcA Fnr |
| title | Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations |
| title_full | Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations |
| title_fullStr | Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations |
| title_full_unstemmed | Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations |
| title_short | Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations |
| title_sort | modeling and simulation of the redox regulation of the metabolism in escherichia coli at different oxygen concentrations |
| topic | Kinetic modeling Fermentation Dissolved oxygen limitation Redox regulation ArcA Fnr |
| url | http://link.springer.com/article/10.1186/s13068-017-0867-0 |
| work_keys_str_mv | AT yumatsuoka modelingandsimulationoftheredoxregulationofthemetabolisminescherichiacoliatdifferentoxygenconcentrations AT hiroyukikurata modelingandsimulationoftheredoxregulationofthemetabolisminescherichiacoliatdifferentoxygenconcentrations |