Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>

Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>

MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i...

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Bibliographic Details
Main Authors: Yanfeng Jia, Quanlin Li, Yuying Li, Wenxue Zhai, Guanghuai Jiang, Chunrong Li
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:International Journal of Molecular Sciences
Subjects:
rice bacterial blight
osa-miR1432
<i>OsCaML2</i>
Online Access:https://www.mdpi.com/1422-0067/22/21/11367
Description
Summary:MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) are still in the early stages. Herein, the expression of osa-miR1432 was induced in resistant genotype IRBB5, but not susceptible genotype IR24, under <i>Xoo</i> strain PXO86 attack. Overexpressed osa-miR1432 heightened rice disease resistance to <i>Xoo</i>, indicated by enhancive enrichment of defense marker genes, raised reactive oxygen species (ROS) levels, repressed bacterial growth and shortened leaf lesion length, whilst the disruptive accumulation of osa-miR1432 accelerated rice susceptibility to <i>Xoo</i> infection. Noticeably, <i>OsCaML2</i> (<i>LOC_Os03g59770</i>) was experimentally confirmed as a target gene of osa-miR1432, and the overexpressing <i>OsCaML2</i> transgenic plants exhibited compromised resistance to <i>Xoo</i> infestation. Our results indicate that osa-miR1432 and <i>OsCaML2</i> were differently responsive to <i>Xoo</i> invasion at the transcriptional level and fine-tune rice resistance to <i>Xoo</i> infection, which may be referable in resistance gene discovery and valuable in the pursuit of improving <i>Xoo</i> resistance in rice breeding.
ISSN:1661-6596
1422-0067

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