Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grow...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/9/882 |
| _version_ | 1797552923017740288 |
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| author | István Grexa Tamás Fekete Judit Molnár Kinga Molnár Gaszton Vizsnyiczai Pál Ormos Lóránd Kelemen |
| author_facet | István Grexa Tamás Fekete Judit Molnár Kinga Molnár Gaszton Vizsnyiczai Pál Ormos Lóránd Kelemen |
| author_sort | István Grexa |
| collection | DOAJ |
| description | A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments. |
| first_indexed | 2024-03-10T16:07:59Z |
| format | Article |
| id | doaj.art-b3a6b577bef84c59813fc7d79940af1b |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T16:07:59Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-b3a6b577bef84c59813fc7d79940af1b2023-11-20T14:43:40ZengMDPI AGMicromachines2072-666X2020-09-0111988210.3390/mi11090882Single-Cell Elasticity Measurement with an Optically Actuated MicrorobotIstván Grexa0Tamás Fekete1Judit Molnár2Kinga Molnár3Gaszton Vizsnyiczai4Pál Ormos5Lóránd Kelemen6Biological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryBiological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryBiological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryBiological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryBiological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryBiological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryBiological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryA cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.https://www.mdpi.com/2072-666X/11/9/882cell elasticityendothelial cellsoptical micromanipulationholographic optical tweezerstwo-photon polymerizationimage processing |
| spellingShingle | István Grexa Tamás Fekete Judit Molnár Kinga Molnár Gaszton Vizsnyiczai Pál Ormos Lóránd Kelemen Single-Cell Elasticity Measurement with an Optically Actuated Microrobot Micromachines cell elasticity endothelial cells optical micromanipulation holographic optical tweezers two-photon polymerization image processing |
| title | Single-Cell Elasticity Measurement with an Optically Actuated Microrobot |
| title_full | Single-Cell Elasticity Measurement with an Optically Actuated Microrobot |
| title_fullStr | Single-Cell Elasticity Measurement with an Optically Actuated Microrobot |
| title_full_unstemmed | Single-Cell Elasticity Measurement with an Optically Actuated Microrobot |
| title_short | Single-Cell Elasticity Measurement with an Optically Actuated Microrobot |
| title_sort | single cell elasticity measurement with an optically actuated microrobot |
| topic | cell elasticity endothelial cells optical micromanipulation holographic optical tweezers two-photon polymerization image processing |
| url | https://www.mdpi.com/2072-666X/11/9/882 |
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