Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence
Fast, reliable methods for characterizing the micelle-to-gel transition in emerging Pluronic F127/polysaccharide materials are essential for tailoring their applications as in situ gelling delivery systems. This study describes a simple fluorimetric method based on the response to gelation of the mo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/12/939 |
| _version_ | 1827574715620786176 |
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| author | George-Alin Balan Aurica Precupas Iulia Matei |
| author_facet | George-Alin Balan Aurica Precupas Iulia Matei |
| author_sort | George-Alin Balan |
| collection | DOAJ |
| description | Fast, reliable methods for characterizing the micelle-to-gel transition in emerging Pluronic F127/polysaccharide materials are essential for tailoring their applications as in situ gelling delivery systems. This study describes a simple fluorimetric method based on the response to gelation of the molecular probe thioflavin T (ThT). The techniques employed are (second derivative) steady-state and synchronous fluorescence. The capabilities of ThT as gelation reporter are tested for three model systems: Pluronic F127 (P16.6%), Pluronic F127/alginate (P16.6%ALG2%) and Pluronic F127/hyaluronic acid (P16.6%HA0.5%). We demonstrate that the changes in the short and long wavelength emissions of ThT allow accurate determination of the critical gelation temperatures in the investigated systems. The spectroscopic data providing information at molecular level are complemented with differential scanning microcalorimetric results revealing additional macroscopic insight into the micellization process. The gelation study is preceded by a solvatochromic analysis of ThT. |
| first_indexed | 2024-03-08T20:45:01Z |
| format | Article |
| id | doaj.art-b3a6dcb7ddfd4e5b9776d12a4418659e |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-08T20:45:01Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-b3a6dcb7ddfd4e5b9776d12a4418659e2023-12-22T14:10:40ZengMDPI AGGels2310-28612023-11-0191293910.3390/gels9120939Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T FluorescenceGeorge-Alin Balan0Aurica Precupas1Iulia Matei2“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, RomaniaFast, reliable methods for characterizing the micelle-to-gel transition in emerging Pluronic F127/polysaccharide materials are essential for tailoring their applications as in situ gelling delivery systems. This study describes a simple fluorimetric method based on the response to gelation of the molecular probe thioflavin T (ThT). The techniques employed are (second derivative) steady-state and synchronous fluorescence. The capabilities of ThT as gelation reporter are tested for three model systems: Pluronic F127 (P16.6%), Pluronic F127/alginate (P16.6%ALG2%) and Pluronic F127/hyaluronic acid (P16.6%HA0.5%). We demonstrate that the changes in the short and long wavelength emissions of ThT allow accurate determination of the critical gelation temperatures in the investigated systems. The spectroscopic data providing information at molecular level are complemented with differential scanning microcalorimetric results revealing additional macroscopic insight into the micellization process. The gelation study is preceded by a solvatochromic analysis of ThT.https://www.mdpi.com/2310-2861/9/12/939thioflavin TPluronic F127alginatehyaluronic acidsteady-state fluorescencesynchronous fluorescence |
| spellingShingle | George-Alin Balan Aurica Precupas Iulia Matei Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence Gels thioflavin T Pluronic F127 alginate hyaluronic acid steady-state fluorescence synchronous fluorescence |
| title | Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence |
| title_full | Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence |
| title_fullStr | Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence |
| title_full_unstemmed | Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence |
| title_short | Gelation Behaviour of Pluronic F127/Polysaccharide Systems Revealed via Thioflavin T Fluorescence |
| title_sort | gelation behaviour of pluronic f127 polysaccharide systems revealed via thioflavin t fluorescence |
| topic | thioflavin T Pluronic F127 alginate hyaluronic acid steady-state fluorescence synchronous fluorescence |
| url | https://www.mdpi.com/2310-2861/9/12/939 |
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