Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda
The fall armyworm (FAW) Spodoptera frugiperda is a destructive and polyphagous pest of many essential food crops including maize and rice. The FAW is hard to manage, control, or eradicate, due to its polyphagous nature and voracity of feeding. Here, we report the characterization and functional anal...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1079442/full |
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| author | Muhammad Hafeez Muhammad Hafeez Xiaowei Li Limin Chen Limin Chen Farman Ullah Jun Huang Zhijun Zhang Jinming Zhang Junaid Ali Siddiqui Shu-xing Zhou Xiao-yun Ren Muhammad Imran Mohammed A. Assiri Yonggen Lou Yaobin Lu |
| author_facet | Muhammad Hafeez Muhammad Hafeez Xiaowei Li Limin Chen Limin Chen Farman Ullah Jun Huang Zhijun Zhang Jinming Zhang Junaid Ali Siddiqui Shu-xing Zhou Xiao-yun Ren Muhammad Imran Mohammed A. Assiri Yonggen Lou Yaobin Lu |
| author_sort | Muhammad Hafeez |
| collection | DOAJ |
| description | The fall armyworm (FAW) Spodoptera frugiperda is a destructive and polyphagous pest of many essential food crops including maize and rice. The FAW is hard to manage, control, or eradicate, due to its polyphagous nature and voracity of feeding. Here, we report the characterization and functional analysis of the detoxification gene CYP302A1 and how S. frugieprda larvae use a detoxification mechanism to adapt host plants. Results demonstrated that CYP302A1 expression levels were much higher in midgut tissue and the older S. frugiperda larvae. Our current studies revealed the enhanced P450 activity in the midguts of S. frugiperda larvae after exposure to rice plants as compared to corn plants and an artificial diet. Furthermore, higher mortality was observed in PBO treated larvae followed by the exposure of rice plants as compared to the corn plant. The dsRNA-fed larvae showed downregulation of CYP302A1 gene in the midgut. At the same time, higher mortality, reduced larval weight and shorter developmental time was observed in the dsRNA-fed larvae followed by the exposure of rice plant as compared to the corn plant and DEPC-water treated plants as a control. These results concluded that the inducible P450 enzyme system and related genes could provide herbivores with an ecological opportunity to adapt to diverse host plants by utilizing secondary compounds present in their host plants. |
| first_indexed | 2024-04-10T20:25:37Z |
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| id | doaj.art-b3a73b9ba8a64f0f81ba1d081ced7c73 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T20:25:37Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b3a73b9ba8a64f0f81ba1d081ced7c732023-01-25T12:17:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10794421079442Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprdaMuhammad Hafeez0Muhammad Hafeez1Xiaowei Li2Limin Chen3Limin Chen4Farman Ullah5Jun Huang6Zhijun Zhang7Jinming Zhang8Junaid Ali Siddiqui9Shu-xing Zhou10Xiao-yun Ren11Muhammad Imran12Mohammed A. Assiri13Yonggen Lou14Yaobin Lu15State Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaIntegrated Plant Protection Center, Lishui Academy of Agricultural and Forestry Sciences, Lishui, ChinaDepartment of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaCollege of Agriculture, College of Tobacco Science, Guizhou University, Guiyang, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaDepartment of Chemistry, Faculty of Science, King Khalid University, Abha, Saudi ArabiaDepartment of Chemistry, Faculty of Science, King Khalid University, Abha, Saudi ArabiaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaThe fall armyworm (FAW) Spodoptera frugiperda is a destructive and polyphagous pest of many essential food crops including maize and rice. The FAW is hard to manage, control, or eradicate, due to its polyphagous nature and voracity of feeding. Here, we report the characterization and functional analysis of the detoxification gene CYP302A1 and how S. frugieprda larvae use a detoxification mechanism to adapt host plants. Results demonstrated that CYP302A1 expression levels were much higher in midgut tissue and the older S. frugiperda larvae. Our current studies revealed the enhanced P450 activity in the midguts of S. frugiperda larvae after exposure to rice plants as compared to corn plants and an artificial diet. Furthermore, higher mortality was observed in PBO treated larvae followed by the exposure of rice plants as compared to the corn plant. The dsRNA-fed larvae showed downregulation of CYP302A1 gene in the midgut. At the same time, higher mortality, reduced larval weight and shorter developmental time was observed in the dsRNA-fed larvae followed by the exposure of rice plant as compared to the corn plant and DEPC-water treated plants as a control. These results concluded that the inducible P450 enzyme system and related genes could provide herbivores with an ecological opportunity to adapt to diverse host plants by utilizing secondary compounds present in their host plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.1079442/fullP450 detoxification enzymespodoptera frugiperdaCYP302a1host adaptationRNAi |
| spellingShingle | Muhammad Hafeez Muhammad Hafeez Xiaowei Li Limin Chen Limin Chen Farman Ullah Jun Huang Zhijun Zhang Jinming Zhang Junaid Ali Siddiqui Shu-xing Zhou Xiao-yun Ren Muhammad Imran Mohammed A. Assiri Yonggen Lou Yaobin Lu Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda Frontiers in Plant Science P450 detoxification enzyme spodoptera frugiperda CYP302a1 host adaptation RNAi |
| title | Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda |
| title_full | Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda |
| title_fullStr | Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda |
| title_full_unstemmed | Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda |
| title_short | Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda |
| title_sort | molecular characterization and functional analysis of cytochrome p450 mediated detoxification cyp302a1 gene involved in host plant adaptation in spodoptera frugieprda |
| topic | P450 detoxification enzyme spodoptera frugiperda CYP302a1 host adaptation RNAi |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1079442/full |
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