INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT
The aim of the work was to study the induction of antimicrobial activity of macromycetes by low-intensity light of different wavelengths and coherence. The objects of investigation were the strains of Flammulina velutipes 3923, Pleurotus ostreatus 531, Ganoderma lucidum 1908 and G. applanatum 1552 f...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-02-01
|
| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/no1_2015/poyedinok_1_2015_.pdf |
| _version_ | 1797728090765393920 |
|---|---|
| author | N. L. Poyedinok O. B. Mykhaylova A. M. Negriyko I. A. Dudka B. F. Vasilyeva O. V. Efremenkova O. V. Efremenkova |
| author_facet | N. L. Poyedinok O. B. Mykhaylova A. M. Negriyko I. A. Dudka B. F. Vasilyeva O. V. Efremenkova O. V. Efremenkova |
| author_sort | N. L. Poyedinok |
| collection | DOAJ |
| description | The aim of the work was to study the induction of antimicrobial activity of macromycetes by low-intensity light of different wavelengths and coherence. The objects of investigation were the strains of Flammulina velutipes 3923, Pleurotus ostreatus 531, Ganoderma lucidum 1908 and G. applanatum 1552 from Mushrooms Collection of the Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, the test-cultures from Cultural Collections of the Gause Institute of New Antibiotics, All-Union Research Institute of Antibiotics and the All-Russian Collection of Industrial microorganisms. Helium-neon laser with a wavelength of 632.8 nm and an argon ion laser with wavelengths of 488.0 nm and 514.5 nm were used as a source of coherent visible light lasers. For obtaining incoherent light LEDs with emission at a wavelength of 490.0, 520.0 and 634.0 nm were used. It was found that short-term exposure of sowing mycelium by low intensity light with the energy density of 230 MJ/cm2 in the red and blue wavelength ranges reduced the cultivation period before the appearance of antimicrobial activity and induced the increasing of the culture fluid inhibitory activity against different test-cultures from 20 to 238%. Selected modes of antimicrobial activity photostimulation could be used in biotechnology of submerged cultivation of macromycetes for intensification of technological stages and increasing the yield of the final product. |
| first_indexed | 2024-03-12T11:08:51Z |
| format | Article |
| id | doaj.art-b3a8b833a5714017aa61f44d4b82a10f |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T11:08:51Z |
| publishDate | 2015-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-b3a8b833a5714017aa61f44d4b82a10f2023-09-02T03:18:38ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2015-02-0181637910.15407/biotech8.01.063INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHTN. L. Poyedinok 0O. B. Mykhaylova1 A. M. Negriyko2I. A. Dudka3 B. F. Vasilyeva4 O. V. Efremenkova5 O. V. Efremenkova6Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, Kyiv, UkraineKholodny Botany Institute of the National Academy of Sciences of Ukraine, Kyiv, UkraineA. M. NegriykoKholodny Botany Institute of the National Academy of Sciences of Ukraine, Kyiv, UkraineGause Institute of New Antibiotics, Moscow, Russian FederationGause Institute of New Antibiotics, Moscow, Russian FederationGause Institute of New Antibiotics, Moscow, Russian FederationThe aim of the work was to study the induction of antimicrobial activity of macromycetes by low-intensity light of different wavelengths and coherence. The objects of investigation were the strains of Flammulina velutipes 3923, Pleurotus ostreatus 531, Ganoderma lucidum 1908 and G. applanatum 1552 from Mushrooms Collection of the Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, the test-cultures from Cultural Collections of the Gause Institute of New Antibiotics, All-Union Research Institute of Antibiotics and the All-Russian Collection of Industrial microorganisms. Helium-neon laser with a wavelength of 632.8 nm and an argon ion laser with wavelengths of 488.0 nm and 514.5 nm were used as a source of coherent visible light lasers. For obtaining incoherent light LEDs with emission at a wavelength of 490.0, 520.0 and 634.0 nm were used. It was found that short-term exposure of sowing mycelium by low intensity light with the energy density of 230 MJ/cm2 in the red and blue wavelength ranges reduced the cultivation period before the appearance of antimicrobial activity and induced the increasing of the culture fluid inhibitory activity against different test-cultures from 20 to 238%. Selected modes of antimicrobial activity photostimulation could be used in biotechnology of submerged cultivation of macromycetes for intensification of technological stages and increasing the yield of the final product.http://biotechnology.kiev.ua/images/storage/no1_2015/poyedinok_1_2015_.pdfmacromyceteslow intensity lightslasersantimicrobial activitylight emitting diodes |
| spellingShingle | N. L. Poyedinok O. B. Mykhaylova A. M. Negriyko I. A. Dudka B. F. Vasilyeva O. V. Efremenkova O. V. Efremenkova INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT Biotechnologia Acta macromycetes low intensity lights lasers antimicrobial activity light emitting diodes |
| title | INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT |
| title_full | INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT |
| title_fullStr | INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT |
| title_full_unstemmed | INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT |
| title_short | INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT |
| title_sort | induction of antimicrobial activity of some macromycetes by low intensity light |
| topic | macromycetes low intensity lights lasers antimicrobial activity light emitting diodes |
| url | http://biotechnology.kiev.ua/images/storage/no1_2015/poyedinok_1_2015_.pdf |
| work_keys_str_mv | AT nlpoyedinok inductionofantimicrobialactivityofsomemacromycetesbylowintensitylight AT obmykhaylova inductionofantimicrobialactivityofsomemacromycetesbylowintensitylight AT amnegriyko inductionofantimicrobialactivityofsomemacromycetesbylowintensitylight AT iadudka inductionofantimicrobialactivityofsomemacromycetesbylowintensitylight AT bfvasilyeva inductionofantimicrobialactivityofsomemacromycetesbylowintensitylight AT ovefremenkova inductionofantimicrobialactivityofsomemacromycetesbylowintensitylight AT ovefremenkova inductionofantimicrobialactivityofsomemacromycetesbylowintensitylight |