An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts
The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-05-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506117300491 |
| _version_ | 1819085373104455680 |
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| author | Enrique Sosa Rachel Kim Ernesto J. Rojas Linzi Hosohama Jon D. Hennebold Kyle E. Orwig Amander T. Clark |
| author_facet | Enrique Sosa Rachel Kim Ernesto J. Rojas Linzi Hosohama Jon D. Hennebold Kyle E. Orwig Amander T. Clark |
| author_sort | Enrique Sosa |
| collection | DOAJ |
| description | The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016). |
| first_indexed | 2024-12-21T21:03:19Z |
| format | Article |
| id | doaj.art-b3a901b5038e480898ed2b4f395bb375 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-21T21:03:19Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-b3a901b5038e480898ed2b4f395bb3752022-12-21T18:50:24ZengElsevierStem Cell Research1873-50611876-77532017-05-0121C5810.1016/j.scr.2017.03.011An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblastsEnrique Sosa0Rachel Kim1Ernesto J. Rojas2Linzi Hosohama3Jon D. Hennebold4Kyle E. Orwig5Amander T. Clark6Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97006, USADepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womans Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USADepartment of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USAThe rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).http://www.sciencedirect.com/science/article/pii/S1873506117300491 |
| spellingShingle | Enrique Sosa Rachel Kim Ernesto J. Rojas Linzi Hosohama Jon D. Hennebold Kyle E. Orwig Amander T. Clark An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts Stem Cell Research |
| title | An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts |
| title_full | An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts |
| title_fullStr | An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts |
| title_full_unstemmed | An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts |
| title_short | An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts |
| title_sort | integration free virus free rhesus macaque induced pluripotent stem cell line ripsc90 from embryonic fibroblasts |
| url | http://www.sciencedirect.com/science/article/pii/S1873506117300491 |
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