1,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory Conditions
Neuroinflammation is one of the common features in most neurological diseases including multiple sclerosis (MScl) and neurodegenerative diseases such as Alzheimer’s disease (AD). It is associated with local brain inflammation, microglial activation, and infiltration of peripheral immune cells into c...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/1/51 |
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| author | Ginevra Giacomello Carolin Otto Josef Priller Klemens Ruprecht Chotima Böttcher Maria Kristina Parr |
| author_facet | Ginevra Giacomello Carolin Otto Josef Priller Klemens Ruprecht Chotima Böttcher Maria Kristina Parr |
| author_sort | Ginevra Giacomello |
| collection | DOAJ |
| description | Neuroinflammation is one of the common features in most neurological diseases including multiple sclerosis (MScl) and neurodegenerative diseases such as Alzheimer’s disease (AD). It is associated with local brain inflammation, microglial activation, and infiltration of peripheral immune cells into cerebrospinal fluid (CSF) and the central nervous system (CNS). It has been shown that the diversity of phenotypic changes in monocytes in CSF relates to neuroinflammation. It remains to be investigated whether these phenotypic changes are associated with functional or metabolic alteration, which may give a hint to their function or changes in cell states, e.g., cell activation. In this article, we investigate whether major metabolic pathways of blood monocytes alter after exposure to CSF of healthy individuals or patients with AD or MScl. Our findings show a significant alteration of the metabolism of monocytes treated with CSF from patients and healthy donors, including higher production of citric acid and glutamine, suggesting a more active glycolysis and tricarboxylic acid (TCA) cycle and reduced production of glycine and serine. These alterations suggest metabolic reprogramming of monocytes, possibly related to the change of compartment (from blood to CSF) and/or disease-related. Moreover, the levels of serine differ between AD and MScl, suggesting different phenotypic alterations between diseases. |
| first_indexed | 2024-03-09T13:07:56Z |
| format | Article |
| id | doaj.art-b3a97dfc95bf43c7b8a2ab1dbd6596b7 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T13:07:56Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-b3a97dfc95bf43c7b8a2ab1dbd6596b72023-11-30T21:45:42ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-01-0145176578110.3390/cimb450100511,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory ConditionsGinevra Giacomello0Carolin Otto1Josef Priller2Klemens Ruprecht3Chotima Böttcher4Maria Kristina Parr5Institute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2 + 4, 14195 Berlin, GermanyDepartment of Neurology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyDepartment of Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyDepartment of Neurology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyDepartment of Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, GermanyInstitute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2 + 4, 14195 Berlin, GermanyNeuroinflammation is one of the common features in most neurological diseases including multiple sclerosis (MScl) and neurodegenerative diseases such as Alzheimer’s disease (AD). It is associated with local brain inflammation, microglial activation, and infiltration of peripheral immune cells into cerebrospinal fluid (CSF) and the central nervous system (CNS). It has been shown that the diversity of phenotypic changes in monocytes in CSF relates to neuroinflammation. It remains to be investigated whether these phenotypic changes are associated with functional or metabolic alteration, which may give a hint to their function or changes in cell states, e.g., cell activation. In this article, we investigate whether major metabolic pathways of blood monocytes alter after exposure to CSF of healthy individuals or patients with AD or MScl. Our findings show a significant alteration of the metabolism of monocytes treated with CSF from patients and healthy donors, including higher production of citric acid and glutamine, suggesting a more active glycolysis and tricarboxylic acid (TCA) cycle and reduced production of glycine and serine. These alterations suggest metabolic reprogramming of monocytes, possibly related to the change of compartment (from blood to CSF) and/or disease-related. Moreover, the levels of serine differ between AD and MScl, suggesting different phenotypic alterations between diseases.https://www.mdpi.com/1467-3045/45/1/51monocytesmetabolismcerebrospinal fluidglycolysistricarboxylic acid cyclemultiple sclerosis |
| spellingShingle | Ginevra Giacomello Carolin Otto Josef Priller Klemens Ruprecht Chotima Böttcher Maria Kristina Parr 1,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory Conditions Current Issues in Molecular Biology monocytes metabolism cerebrospinal fluid glycolysis tricarboxylic acid cycle multiple sclerosis |
| title | 1,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory Conditions |
| title_full | 1,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory Conditions |
| title_fullStr | 1,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory Conditions |
| title_full_unstemmed | 1,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory Conditions |
| title_short | 1,2-<sup>13</sup>C<sub>2</sub>-Glucose Tracing Approach to Assess Metabolic Alterations of Human Monocytes under Neuroinflammatory Conditions |
| title_sort | 1 2 sup 13 sup c sub 2 sub glucose tracing approach to assess metabolic alterations of human monocytes under neuroinflammatory conditions |
| topic | monocytes metabolism cerebrospinal fluid glycolysis tricarboxylic acid cycle multiple sclerosis |
| url | https://www.mdpi.com/1467-3045/45/1/51 |
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