Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen
The P. longifolia mediated silver (PL-AgNPs) nanoparticles are very stable and efficient. UV–Vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive X-ray spectroscopy (EDX) were used...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580822001200 |
| _version_ | 1828758412032212992 |
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| author | Alankrita Dashora Kavita Rathore Shani Raj Kanika Sharma |
| author_facet | Alankrita Dashora Kavita Rathore Shani Raj Kanika Sharma |
| author_sort | Alankrita Dashora |
| collection | DOAJ |
| description | The P. longifolia mediated silver (PL-AgNPs) nanoparticles are very stable and efficient. UV–Vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive X-ray spectroscopy (EDX) were used to characterize the produced AgNPs. UV–Vis analysis showed a characteristic peak at 435 nm corresponding to surface plasmon resonance. The synthesis process was spectrophotometrically optimized for various parameters. After optimization, highly stable AgNPs were prepared using 3.0 ml of P. longifolia leaf extract, pH 7.0, 1.0 mM AgNO3, and 60 °C. The zeta potential was measured by DLS, which showed −20.8 mV and the PDI value was 5.42. TEM and SEM analysis shows a spherical shape of the synthesized nanoparticles, and the size was measured between 10 and 40 nm. EDX analysis showed intense peaks from silver and oxygen and small peaks from various metal atoms such as Na, P, S and Al indicating their presence in trace amounts. The average size of the PL-AgNPs was 14 nm. The phytochemical analysis shows that the presence of alkaloids, essential oils and saponins seems to be responsible for the synthesis of nanoparticles. PL-AgNPs were further investigated for their antifungal activity against Alternaria alternata. The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and effect of nanoparticles on cytomorphology of A. alternata have also been reported. Biosynthesized nanoparticles have proven to be inexpensive, environmentally friendly, stable, easily reproducible, and highly effective against plant-pathogenic fungi. |
| first_indexed | 2024-12-11T00:41:25Z |
| format | Article |
| id | doaj.art-b3aad12982954ba38cd7369209bda8e0 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-11T00:41:25Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-b3aad12982954ba38cd7369209bda8e02022-12-22T01:26:54ZengElsevierBiochemistry and Biophysics Reports2405-58082022-09-0131101320Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogenAlankrita Dashora0Kavita Rathore1Shani Raj2Kanika Sharma3Department of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaDepartment of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaCorresponding author.; Department of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaDepartment of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaThe P. longifolia mediated silver (PL-AgNPs) nanoparticles are very stable and efficient. UV–Vis spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive X-ray spectroscopy (EDX) were used to characterize the produced AgNPs. UV–Vis analysis showed a characteristic peak at 435 nm corresponding to surface plasmon resonance. The synthesis process was spectrophotometrically optimized for various parameters. After optimization, highly stable AgNPs were prepared using 3.0 ml of P. longifolia leaf extract, pH 7.0, 1.0 mM AgNO3, and 60 °C. The zeta potential was measured by DLS, which showed −20.8 mV and the PDI value was 5.42. TEM and SEM analysis shows a spherical shape of the synthesized nanoparticles, and the size was measured between 10 and 40 nm. EDX analysis showed intense peaks from silver and oxygen and small peaks from various metal atoms such as Na, P, S and Al indicating their presence in trace amounts. The average size of the PL-AgNPs was 14 nm. The phytochemical analysis shows that the presence of alkaloids, essential oils and saponins seems to be responsible for the synthesis of nanoparticles. PL-AgNPs were further investigated for their antifungal activity against Alternaria alternata. The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and effect of nanoparticles on cytomorphology of A. alternata have also been reported. Biosynthesized nanoparticles have proven to be inexpensive, environmentally friendly, stable, easily reproducible, and highly effective against plant-pathogenic fungi.http://www.sciencedirect.com/science/article/pii/S2405580822001200Silver nanoparticlePolyalthia longifoliaGreen synthesisAntifungal activityCyto-morphology |
| spellingShingle | Alankrita Dashora Kavita Rathore Shani Raj Kanika Sharma Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen Biochemistry and Biophysics Reports Silver nanoparticle Polyalthia longifolia Green synthesis Antifungal activity Cyto-morphology |
| title | Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen |
| title_full | Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen |
| title_fullStr | Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen |
| title_full_unstemmed | Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen |
| title_short | Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen |
| title_sort | synthesis of silver nanoparticles employing polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen |
| topic | Silver nanoparticle Polyalthia longifolia Green synthesis Antifungal activity Cyto-morphology |
| url | http://www.sciencedirect.com/science/article/pii/S2405580822001200 |
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