Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity
Silkworm (Bombyx mori) pupae is a by-product from the silk industry which is rich in protein. Hydrolysates from silkworm pupae generated using Alcalase®, Prolyve®, Flavourzyme® and Brewers Clarex® proteolytic preparations were characterised. The antioxidant activity of the hydrolysates was investiga...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622001220 |
| _version_ | 1828408106773643264 |
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| author | Maria Cermeño Carmen Bascón Miryam Amigo-Benavent Manuel Felix Richard J. FitzGerald |
| author_facet | Maria Cermeño Carmen Bascón Miryam Amigo-Benavent Manuel Felix Richard J. FitzGerald |
| author_sort | Maria Cermeño |
| collection | DOAJ |
| description | Silkworm (Bombyx mori) pupae is a by-product from the silk industry which is rich in protein. Hydrolysates from silkworm pupae generated using Alcalase®, Prolyve®, Flavourzyme® and Brewers Clarex® proteolytic preparations were characterised. The antioxidant activity of the hydrolysates was investigated using in vitro antioxidant assays and an in situ assay for reactive oxygen species (ROS) reduction using hepatic HepG2. Overall, Alcalase and Prolyve hydrolysates had highest scavenging activities, however, Flavourzyme and Brewers Clarex hydrolysates had enhanced ferric reducing antioxidant power (FRAP) activity compared to the other samples. Furthermore, the Flavourzyme hydrolysate significantly reduced ROS by 40% compared to untreated control HepG2 cells. Peptides identified by LC-MS/MS were synthetised and then tested for their in vitro and in situ antioxidant activity. Peptides SWFVTPF and NDVLFF showed highest antioxidant activity (ROS reduction, superoxide dismutase (SOD) expression and glutathione (GSH) production activity) in HepG2 cells, and therefore may have potential as natural antioxidants. |
| first_indexed | 2024-12-10T11:35:02Z |
| format | Article |
| id | doaj.art-b3ab443e28c74f6aa3353fa7c552a12c |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-10T11:35:02Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-b3ab443e28c74f6aa3353fa7c552a12c2022-12-22T01:50:28ZengElsevierJournal of Functional Foods1756-46462022-05-0192105052Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activityMaria Cermeño0Carmen Bascón1Miryam Amigo-Benavent2Manuel Felix3Richard J. FitzGerald4Department of Biological Sciences, University of Limerick, Castletroy, Limerick, IrelandDepartment of Biological Sciences, University of Limerick, Castletroy, Limerick, Ireland; Departamento de Ingeniería Química, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Sevilla, SpainDepartment of Biological Sciences, University of Limerick, Castletroy, Limerick, IrelandDepartamento de Ingeniería Química, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Sevilla, SpainDepartment of Biological Sciences, University of Limerick, Castletroy, Limerick, Ireland; Corresponding author.Silkworm (Bombyx mori) pupae is a by-product from the silk industry which is rich in protein. Hydrolysates from silkworm pupae generated using Alcalase®, Prolyve®, Flavourzyme® and Brewers Clarex® proteolytic preparations were characterised. The antioxidant activity of the hydrolysates was investigated using in vitro antioxidant assays and an in situ assay for reactive oxygen species (ROS) reduction using hepatic HepG2. Overall, Alcalase and Prolyve hydrolysates had highest scavenging activities, however, Flavourzyme and Brewers Clarex hydrolysates had enhanced ferric reducing antioxidant power (FRAP) activity compared to the other samples. Furthermore, the Flavourzyme hydrolysate significantly reduced ROS by 40% compared to untreated control HepG2 cells. Peptides identified by LC-MS/MS were synthetised and then tested for their in vitro and in situ antioxidant activity. Peptides SWFVTPF and NDVLFF showed highest antioxidant activity (ROS reduction, superoxide dismutase (SOD) expression and glutathione (GSH) production activity) in HepG2 cells, and therefore may have potential as natural antioxidants.http://www.sciencedirect.com/science/article/pii/S1756464622001220AntioxidantBioactiveBombyx moriPeptideSilkworm |
| spellingShingle | Maria Cermeño Carmen Bascón Miryam Amigo-Benavent Manuel Felix Richard J. FitzGerald Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity Journal of Functional Foods Antioxidant Bioactive Bombyx mori Peptide Silkworm |
| title | Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity |
| title_full | Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity |
| title_fullStr | Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity |
| title_full_unstemmed | Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity |
| title_short | Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity |
| title_sort | identification of peptides from edible silkworm pupae bombyx mori protein hydrolysates with antioxidant activity |
| topic | Antioxidant Bioactive Bombyx mori Peptide Silkworm |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622001220 |
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