Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal Water
Clostridioides difficile infection (CDI) is frequently associated with intestinal injury and mucosal barrier dysfunction, leading to an inflammatory response involving neutrophil localization and upregulation of pro-inflammatory cytokines. The severity of clinical manifestations is associated with t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.698638/full |
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| author | Mohd Baasir Gaisawat Silvia Lopez-Escalera Chad W. MacPherson Michèle M. Iskandar Thomas A. Tompkins Stan Kubow |
| author_facet | Mohd Baasir Gaisawat Silvia Lopez-Escalera Chad W. MacPherson Michèle M. Iskandar Thomas A. Tompkins Stan Kubow |
| author_sort | Mohd Baasir Gaisawat |
| collection | DOAJ |
| description | Clostridioides difficile infection (CDI) is frequently associated with intestinal injury and mucosal barrier dysfunction, leading to an inflammatory response involving neutrophil localization and upregulation of pro-inflammatory cytokines. The severity of clinical manifestations is associated with the extent of the immune response, which requires mitigation for better clinical management. Probiotics could play a protective role in this disorder due to their immunomodulatory ability in gastrointestinal disorders. We assessed five single-strain and three multi-strain probiotics for their ability to modulate CDI fecal water (FW)-induced effects on T84 cells. The CDI-FW significantly (p < 0.05) decreased T84 cell viability. The CDI-FW-exposed cells also exhibited increased pro-inflammatory cytokine production as characterized by interleukin (IL)-8, C-X-C motif chemokine 5, macrophage inhibitory factor (MIF), IL-32, and tumor necrosis factor (TNF) ligand superfamily member 8. Probiotics were associated with strain-specific attenuation of the CDI-FW mediated effects, whereby Saccharomyces boulardii CNCM I-1079 and Lacticaseibacillus rhamnosus R0011 were most effective in reducing pro-inflammatory cytokine production and in increasing T84 cell viability. ProtecFlor™, Lactobacillus helveticus R0052, and Bifidobacterium longum R0175 showed moderate effectiveness, and L. rhamnosus GG R0343 along with the two other multi-strain combinations were the least effective. Overall, the findings showed that probiotic strains possess the capability to modulate the CDI-mediated inflammatory response in the gut lumen. |
| first_indexed | 2024-12-20T10:25:05Z |
| format | Article |
| id | doaj.art-b3ab93052e014c0a8173566606c2da90 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T10:25:05Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b3ab93052e014c0a8173566606c2da902022-12-21T19:43:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-01-011210.3389/fmicb.2021.698638698638Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal WaterMohd Baasir Gaisawat0Silvia Lopez-Escalera1Chad W. MacPherson2Michèle M. Iskandar3Thomas A. Tompkins4Stan Kubow5School of Human Nutrition, McGill University, Montréal, QC, CanadaDepartment of Nutrigenomics, Wageningen University, Wageningen, NetherlandsRosell Institute for Microbiome and Probiotics, Montréal, QC, CanadaSchool of Human Nutrition, McGill University, Montréal, QC, CanadaRosell Institute for Microbiome and Probiotics, Montréal, QC, CanadaSchool of Human Nutrition, McGill University, Montréal, QC, CanadaClostridioides difficile infection (CDI) is frequently associated with intestinal injury and mucosal barrier dysfunction, leading to an inflammatory response involving neutrophil localization and upregulation of pro-inflammatory cytokines. The severity of clinical manifestations is associated with the extent of the immune response, which requires mitigation for better clinical management. Probiotics could play a protective role in this disorder due to their immunomodulatory ability in gastrointestinal disorders. We assessed five single-strain and three multi-strain probiotics for their ability to modulate CDI fecal water (FW)-induced effects on T84 cells. The CDI-FW significantly (p < 0.05) decreased T84 cell viability. The CDI-FW-exposed cells also exhibited increased pro-inflammatory cytokine production as characterized by interleukin (IL)-8, C-X-C motif chemokine 5, macrophage inhibitory factor (MIF), IL-32, and tumor necrosis factor (TNF) ligand superfamily member 8. Probiotics were associated with strain-specific attenuation of the CDI-FW mediated effects, whereby Saccharomyces boulardii CNCM I-1079 and Lacticaseibacillus rhamnosus R0011 were most effective in reducing pro-inflammatory cytokine production and in increasing T84 cell viability. ProtecFlor™, Lactobacillus helveticus R0052, and Bifidobacterium longum R0175 showed moderate effectiveness, and L. rhamnosus GG R0343 along with the two other multi-strain combinations were the least effective. Overall, the findings showed that probiotic strains possess the capability to modulate the CDI-mediated inflammatory response in the gut lumen.https://www.frontiersin.org/articles/10.3389/fmicb.2021.698638/fullprobioticsT84 cellsC. difficileinflammationcytokinescytotoxicity |
| spellingShingle | Mohd Baasir Gaisawat Silvia Lopez-Escalera Chad W. MacPherson Michèle M. Iskandar Thomas A. Tompkins Stan Kubow Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal Water Frontiers in Microbiology probiotics T84 cells C. difficile inflammation cytokines cytotoxicity |
| title | Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal Water |
| title_full | Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal Water |
| title_fullStr | Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal Water |
| title_full_unstemmed | Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal Water |
| title_short | Probiotics Exhibit Strain-Specific Protective Effects in T84 Cells Challenged With Clostridioides difficile-Infected Fecal Water |
| title_sort | probiotics exhibit strain specific protective effects in t84 cells challenged with clostridioides difficile infected fecal water |
| topic | probiotics T84 cells C. difficile inflammation cytokines cytotoxicity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.698638/full |
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