Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS
To determine the composition and protective effect on Raw 264.7 cells of polysaccharide from Pseudostellaria heterophylla as a traditional Chinese medicinal, the main active ingredient-Pseudostellaria heterophylla polysaccharide (PHP) was extracted by water, precipitated by ethanol, and purified by...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-07-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100292 |
| _version_ | 1798017939001049088 |
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| author | Meilin XIE Haiqing XU Jingming LI Daoxi ZHA Xueying WANG Yang CHEN Qifa ZHU |
| author_facet | Meilin XIE Haiqing XU Jingming LI Daoxi ZHA Xueying WANG Yang CHEN Qifa ZHU |
| author_sort | Meilin XIE |
| collection | DOAJ |
| description | To determine the composition and protective effect on Raw 264.7 cells of polysaccharide from Pseudostellaria heterophylla as a traditional Chinese medicinal, the main active ingredient-Pseudostellaria heterophylla polysaccharide (PHP) was extracted by water, precipitated by ethanol, and purified by anion exchange column chromatography. Gel permeation chromatography (GPC) and high performance liquid chromatography (HPLC) were used to determine molecular weight and monosaccharide composition of PHP. Ultraviolet spectroscopy (UV) and Fourier transform infrared spectroscopy (FT-IR) were used for primary structural characterization of PHP. Furthermore, the Raw 264.7 cell model was used to explore protective effect of PHP on Raw 264.7 cells induced by LPS. The results showed that the yield of crude polysaccharide of Pseudostellaria heterophylla was 8.23%, and the polysaccharide content was 51.47%. After purification by DEAE-52 column chromatography, the polysaccharide content increased to 80.52%, and the molecular weight distribution was between 500 to 92×103 Da. The monosaccharide components of PHP include glucose, galactose, arabinose, mannose and glucuronic acid. Cell experiments showed that the safe dose of PHP was 80~640 µg/mL and could increase the survival rate of cells induced by lipopolysaccharide (LPS). At a dose of 640 µg/mL, the survival rate increased by 7.21%, and it was found that PHP could improve the morphology of Raw 264.7 cells. In summary, in this study the polysaccharides from Pseudostellaria heterophylla were purified, the structure of PHP was characterized, and it was preliminarily confirmed that PHP had potential protective effect on LPS-induced damage to Raw 264.7 cells. This laid the foundation for the follow-up exploration of functional activity, structure-effect and dose-effect of PHP. |
| first_indexed | 2024-04-11T16:15:49Z |
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| id | doaj.art-b3ad24d09e4d49e9aeb376f850514186 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:15:49Z |
| publishDate | 2022-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-b3ad24d09e4d49e9aeb376f8505141862022-12-22T04:14:34ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-07-01431439240010.13386/j.issn1002-0306.20211002922021100292-14Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPSMeilin XIE0Haiqing XU1Jingming LI2Daoxi ZHA3Xueying WANG4Yang CHEN5Qifa ZHU6College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaAnhui Wannan Tobacco Leaf Co., Ltd., Xuancheng 242000, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaAnhui Wannan Tobacco Leaf Co., Ltd., Xuancheng 242000, ChinaAnhui Wannan Tobacco Leaf Co., Ltd., Xuancheng 242000, ChinaAnhui Wannan Tobacco Leaf Co., Ltd., Xuancheng 242000, ChinaAnhui Wannan Tobacco Leaf Co., Ltd., Xuancheng 242000, ChinaTo determine the composition and protective effect on Raw 264.7 cells of polysaccharide from Pseudostellaria heterophylla as a traditional Chinese medicinal, the main active ingredient-Pseudostellaria heterophylla polysaccharide (PHP) was extracted by water, precipitated by ethanol, and purified by anion exchange column chromatography. Gel permeation chromatography (GPC) and high performance liquid chromatography (HPLC) were used to determine molecular weight and monosaccharide composition of PHP. Ultraviolet spectroscopy (UV) and Fourier transform infrared spectroscopy (FT-IR) were used for primary structural characterization of PHP. Furthermore, the Raw 264.7 cell model was used to explore protective effect of PHP on Raw 264.7 cells induced by LPS. The results showed that the yield of crude polysaccharide of Pseudostellaria heterophylla was 8.23%, and the polysaccharide content was 51.47%. After purification by DEAE-52 column chromatography, the polysaccharide content increased to 80.52%, and the molecular weight distribution was between 500 to 92×103 Da. The monosaccharide components of PHP include glucose, galactose, arabinose, mannose and glucuronic acid. Cell experiments showed that the safe dose of PHP was 80~640 µg/mL and could increase the survival rate of cells induced by lipopolysaccharide (LPS). At a dose of 640 µg/mL, the survival rate increased by 7.21%, and it was found that PHP could improve the morphology of Raw 264.7 cells. In summary, in this study the polysaccharides from Pseudostellaria heterophylla were purified, the structure of PHP was characterized, and it was preliminarily confirmed that PHP had potential protective effect on LPS-induced damage to Raw 264.7 cells. This laid the foundation for the follow-up exploration of functional activity, structure-effect and dose-effect of PHP.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100292pseudostellaria heterophylla polysaccharidemonosaccharide compositionraw 264.7 cellsprotective effects |
| spellingShingle | Meilin XIE Haiqing XU Jingming LI Daoxi ZHA Xueying WANG Yang CHEN Qifa ZHU Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS Shipin gongye ke-ji pseudostellaria heterophylla polysaccharide monosaccharide composition raw 264.7 cells protective effects |
| title | Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS |
| title_full | Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS |
| title_fullStr | Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS |
| title_full_unstemmed | Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS |
| title_short | Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS |
| title_sort | purification and composition of polysaccharides from pseudostellaria heterophylla and protective effect on raw 264 7 cells induced by lps |
| topic | pseudostellaria heterophylla polysaccharide monosaccharide composition raw 264.7 cells protective effects |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100292 |
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