The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits
Apple ring rot caused by Botryosphaeria dothidea is an essential and prevalent disease in the apple orchard in China. Our previous study demonstrated that dimethyl trisulfide (DT) from Chinese leek (Allium tuberosum) significantly suppressed the mycelial growth of B. dothidea and inhibited the incid...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.930012/full |
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| author | Yonghong Huang Yonghong Huang Yonghong Huang Yonghong Huang Junping Liu Junping Liu Junping Liu Junping Liu Jinghui Li Jinghui Li Jinghui Li Jinghui Li Meng Sun Meng Sun Meng Sun Meng Sun Yanxin Duan Yanxin Duan Yanxin Duan Yanxin Duan |
| author_facet | Yonghong Huang Yonghong Huang Yonghong Huang Yonghong Huang Junping Liu Junping Liu Junping Liu Junping Liu Jinghui Li Jinghui Li Jinghui Li Jinghui Li Meng Sun Meng Sun Meng Sun Meng Sun Yanxin Duan Yanxin Duan Yanxin Duan Yanxin Duan |
| author_sort | Yonghong Huang |
| collection | DOAJ |
| description | Apple ring rot caused by Botryosphaeria dothidea is an essential and prevalent disease in the apple orchard in China. Our previous study demonstrated that dimethyl trisulfide (DT) from Chinese leek (Allium tuberosum) significantly suppressed the mycelial growth of B. dothidea and inhibited the incidence of apple ring rot postharvest. However, the mechanism underlying the inhibitory role of DT against B. dothidea is not fully understood. Comparing the control and the DT-treated B. dothidea mycelial transcriptomes revealed that heat shock protein 20 (Hsp20) strongly responded to DT treatment. This study identified four Hsp20 genes throughout the B. dothidea genome (BdHsp20_1-4). Each BdHsp20 gene had a conserved ACD with a variable N-terminal region and a short C-terminal extension. The segmental duplication event has contributed to the expansion of the BdHsp20 gene family. Compared to the wild-type strain, the CRISPR/Cas9 gene-edited BdHsp20 mutant (ΔBdHsp20) decreased the mycelial growth by 55.95% and reduced the disease symptom in postharvest apple fruit by 96.34%. However, the BdHsp20 complemented strain (ΔBdHsp20_C) significantly restored the growth and pathogenicity, which suggested that the BdHsp20 gene was closely involved in the growth and pathogenicity of B. dothidea. This study would accelerate the exploration of the molecular mechanism of the inhibitory effect of DT against B. dothidea and also provide new insights for the management of apple ring rot disease. |
| first_indexed | 2024-12-10T08:36:19Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T08:36:19Z |
| publishDate | 2022-07-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-b3ad7897845a455c9852a2885b919cc52022-12-22T01:55:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.930012930012The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruitsYonghong Huang0Yonghong Huang1Yonghong Huang2Yonghong Huang3Junping Liu4Junping Liu5Junping Liu6Junping Liu7Jinghui Li8Jinghui Li9Jinghui Li10Jinghui Li11Meng Sun12Meng Sun13Meng Sun14Meng Sun15Yanxin Duan16Yanxin Duan17Yanxin Duan18Yanxin Duan19College of Horticulture, Qingdao Agricultural University, Qingdao, ChinaLaboratory of Quality and Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao, ChinaNational Technology Centre for Whole Process Quality Control of FSEN Horticultural Products (Qingdao), Qingdao, ChinaQingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao, ChinaLaboratory of Quality and Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao, ChinaNational Technology Centre for Whole Process Quality Control of FSEN Horticultural Products (Qingdao), Qingdao, ChinaQingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao, ChinaLaboratory of Quality and Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao, ChinaNational Technology Centre for Whole Process Quality Control of FSEN Horticultural Products (Qingdao), Qingdao, ChinaQingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao, ChinaLaboratory of Quality and Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao, ChinaNational Technology Centre for Whole Process Quality Control of FSEN Horticultural Products (Qingdao), Qingdao, ChinaQingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao, ChinaLaboratory of Quality and Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao, ChinaNational Technology Centre for Whole Process Quality Control of FSEN Horticultural Products (Qingdao), Qingdao, ChinaQingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao, ChinaApple ring rot caused by Botryosphaeria dothidea is an essential and prevalent disease in the apple orchard in China. Our previous study demonstrated that dimethyl trisulfide (DT) from Chinese leek (Allium tuberosum) significantly suppressed the mycelial growth of B. dothidea and inhibited the incidence of apple ring rot postharvest. However, the mechanism underlying the inhibitory role of DT against B. dothidea is not fully understood. Comparing the control and the DT-treated B. dothidea mycelial transcriptomes revealed that heat shock protein 20 (Hsp20) strongly responded to DT treatment. This study identified four Hsp20 genes throughout the B. dothidea genome (BdHsp20_1-4). Each BdHsp20 gene had a conserved ACD with a variable N-terminal region and a short C-terminal extension. The segmental duplication event has contributed to the expansion of the BdHsp20 gene family. Compared to the wild-type strain, the CRISPR/Cas9 gene-edited BdHsp20 mutant (ΔBdHsp20) decreased the mycelial growth by 55.95% and reduced the disease symptom in postharvest apple fruit by 96.34%. However, the BdHsp20 complemented strain (ΔBdHsp20_C) significantly restored the growth and pathogenicity, which suggested that the BdHsp20 gene was closely involved in the growth and pathogenicity of B. dothidea. This study would accelerate the exploration of the molecular mechanism of the inhibitory effect of DT against B. dothidea and also provide new insights for the management of apple ring rot disease.https://www.frontiersin.org/articles/10.3389/fmicb.2022.930012/fullBotryosphaeria dothideadimethyl trisulfideHsp20 gene familyCRISPR/Cas9apple ring rot |
| spellingShingle | Yonghong Huang Yonghong Huang Yonghong Huang Yonghong Huang Junping Liu Junping Liu Junping Liu Junping Liu Jinghui Li Jinghui Li Jinghui Li Jinghui Li Meng Sun Meng Sun Meng Sun Meng Sun Yanxin Duan Yanxin Duan Yanxin Duan Yanxin Duan The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits Frontiers in Microbiology Botryosphaeria dothidea dimethyl trisulfide Hsp20 gene family CRISPR/Cas9 apple ring rot |
| title | The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits |
| title_full | The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits |
| title_fullStr | The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits |
| title_full_unstemmed | The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits |
| title_short | The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits |
| title_sort | heat shock protein 20 gene editing suppresses mycelial growth of botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits |
| topic | Botryosphaeria dothidea dimethyl trisulfide Hsp20 gene family CRISPR/Cas9 apple ring rot |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.930012/full |
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